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Title:
Pooled shRNA Screenings: Experimental Approach | SpringerLink
Description:
RNA interference (RNAi) has emerged as a powerful genetic strategy to functionally interrogate the entire genome by loss-of-function studies. In the last years, several arrayed shRNA libraries that can silence almost all the human genome have been developed. The...
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Keywords {🔍}

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Topics {✒️}

large-scale rna-interference-based screens rna-interference-based functional genomics month download article/chapter large-scale rnai screen high-level transgene expression protocol rodriguez-barrueco vitro-pooled shrna screening pooled shrna screenings pooled shrna screen arrayed shrna libraries pooled shrna screening privacy choices/manage cookies device instant download paddison pj editor information editors efficient shrna plasmids pancreatic cancer powerful genetic strategy rna interference experimental approach protocol lentiviral rnai library profiling essential genes european economic area producing recombinant adeno multiplex rnai screening journal finder publish conditions privacy policy mouse genes applied silence gene expression reverse genetics coming improved lentiviral vectors human hematopoietic progenitors somatic cell genetics silva jm accepting optional cookies main content log differentiated blood lineages shrna library human mammary cells human cells identifies protocol usd 49 silva department author correspondence protocol cite social media entire genome cancer cell 2 permissions reprints cancer genetics pooled fashion

Questions {❓}

Paddison PJ, Hannon GJ (2002) RNA interference: the new somatic cell genetics?

Schema {🗺️}

ScholarlyArticle:
      headline:Pooled shRNA Screenings: Experimental Approach
      pageEnd:370
      pageStart:353
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-62703-287-2.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:Pancreatic Cancer
         isbn:
            978-1-62703-287-2
            978-1-62703-286-5
         type:Book
      publisher:
         name:Humana Press
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Ruth Rodriguez-Barrueco
            affiliation:
                  name:Columbia University
                  address:
                     name:Institute for Cancer Genetics, Columbia University, New York, USA
                     type:PostalAddress
                  type:Organization
                  name:Columbia University
                  address:
                     name:Department of Pathology, Columbia University, New York, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Netonia Marshall
            affiliation:
                  name:Columbia University
                  address:
                     name:Institute for Cancer Genetics, Columbia University, New York, USA
                     type:PostalAddress
                  type:Organization
                  name:Columbia University
                  address:
                     name:Department of Pathology, Columbia University, New York, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Jose M. Silva
            affiliation:
                  name:Columbia University Medical Center
                  address:
                     name:Institute for Cancer Genetics, Columbia University Medical Center, New York, USA
                     type:PostalAddress
                  type:Organization
                  name:Columbia University Medical Center
                  address:
                     name:Department of Pathology, Columbia University Medical Center, New York, USA
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      keywords:Genome-wide, Pooled screen, shRNA
      description:RNA interference (RNAi) has emerged as a powerful genetic strategy to functionally interrogate the entire genome by loss-of-function studies. In the last years, several arrayed shRNA libraries that can silence almost all the human genome have been developed. The generation of new and more efficient shRNA plasmids has allowed performing genetic screens in a pooled fashion and provides the opportunity to investigate the entire genome finding relevant genes to any biological process. In this chapter, the pipeline and methods to perform a pooled shRNA screen are discussed.
      datePublished:2013
      isAccessibleForFree:
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Book:
      name:Pancreatic Cancer
      isbn:
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         978-1-62703-286-5
Organization:
      name:Humana Press
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         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Columbia University
      address:
         name:Institute for Cancer Genetics, Columbia University, New York, USA
         type:PostalAddress
      name:Columbia University
      address:
         name:Department of Pathology, Columbia University, New York, USA
         type:PostalAddress
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      address:
         name:Institute for Cancer Genetics, Columbia University, New York, USA
         type:PostalAddress
      name:Columbia University
      address:
         name:Department of Pathology, Columbia University, New York, USA
         type:PostalAddress
      name:Columbia University Medical Center
      address:
         name:Institute for Cancer Genetics, Columbia University Medical Center, New York, USA
         type:PostalAddress
      name:Columbia University Medical Center
      address:
         name:Department of Pathology, Columbia University Medical Center, New York, USA
         type:PostalAddress
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      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Ruth Rodriguez-Barrueco
      affiliation:
            name:Columbia University
            address:
               name:Institute for Cancer Genetics, Columbia University, New York, USA
               type:PostalAddress
            type:Organization
            name:Columbia University
            address:
               name:Department of Pathology, Columbia University, New York, USA
               type:PostalAddress
            type:Organization
      name:Netonia Marshall
      affiliation:
            name:Columbia University
            address:
               name:Institute for Cancer Genetics, Columbia University, New York, USA
               type:PostalAddress
            type:Organization
            name:Columbia University
            address:
               name:Department of Pathology, Columbia University, New York, USA
               type:PostalAddress
            type:Organization
      name:Jose M. Silva
      affiliation:
            name:Columbia University Medical Center
            address:
               name:Institute for Cancer Genetics, Columbia University Medical Center, New York, USA
               type:PostalAddress
            type:Organization
            name:Columbia University Medical Center
            address:
               name:Department of Pathology, Columbia University Medical Center, New York, USA
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Institute for Cancer Genetics, Columbia University, New York, USA
      name:Department of Pathology, Columbia University, New York, USA
      name:Institute for Cancer Genetics, Columbia University, New York, USA
      name:Department of Pathology, Columbia University, New York, USA
      name:Institute for Cancer Genetics, Columbia University Medical Center, New York, USA
      name:Department of Pathology, Columbia University Medical Center, New York, USA
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