We are analyzing https://link.springer.com/protocol/10.1007/978-1-62703-260-5_3.

Title:
HPLC Purification of In Vitro Transcribed Long RNA | SpringerLink
Description:
In vitro transcription of DNA with phage RNA polymerases is currently the most efficient method to produce long sequence-specific RNA. While the reaction can yield large quantities of RNA, it contains impurities due to various unwanted activities of the polymerases....
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Keywords {🔍}

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Topics {✒️}

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Schema {🗺️}

ScholarlyArticle:
      headline:HPLC Purification of In Vitro Transcribed Long RNA
      pageEnd:54
      pageStart:43
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-62703-260-5.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:Synthetic Messenger RNA and Cell Metabolism Modulation
         isbn:
            978-1-62703-260-5
            978-1-62703-259-9
         type:Book
      publisher:
         name:Humana Press
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Drew Weissman
            affiliation:
                  name:University of Pennsylvania
                  address:
                     name:Department of Medicine, University of Pennsylvania, Philadelphia, USA
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
            name:Norbert Pardi
            affiliation:
                  name:University of Pennsylvania
                  address:
                     name:Department of Medicine, University of Pennsylvania, Philadelphia, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Hiro Muramatsu
            affiliation:
                  name:University of Pennsylvania
                  address:
                     name:Department of Neurosurgery, University of Pennsylvania, Philadelphia, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Katalin Karikó
            affiliation:
                  name:University of Pennsylvania
                  address:
                     name:Department of Neurosurgery, University of Pennsylvania, Philadelphia, USA
                     type:PostalAddress
                  type:Organization
            type:Person
      keywords:RNA, In vitro transcription, Nucleoside modification, HPLC
      description:In vitro transcription of DNA with phage RNA polymerases is currently the most efficient method to produce long sequence-specific RNA. While the reaction can yield large quantities of RNA, it contains impurities due to various unwanted activities of the polymerases. Here, we described an easily performed HPLC purification that removes multiple contaminants from in vitro transcribed RNA and is scalable. The purified RNA is translated at much greater levels, especially in primary cells and in vivo. HPLC purification of RNA containing modified nucleosides that suppress RNA-mediated activation of innate immune sensors leads to a non-immunogenic RNA with superior translational capacity.
      datePublished:2013
      isAccessibleForFree:
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Book:
      name:Synthetic Messenger RNA and Cell Metabolism Modulation
      isbn:
         978-1-62703-260-5
         978-1-62703-259-9
Organization:
      name:Humana Press
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:University of Pennsylvania
      address:
         name:Department of Medicine, University of Pennsylvania, Philadelphia, USA
         type:PostalAddress
      name:University of Pennsylvania
      address:
         name:Department of Medicine, University of Pennsylvania, Philadelphia, USA
         type:PostalAddress
      name:University of Pennsylvania
      address:
         name:Department of Neurosurgery, University of Pennsylvania, Philadelphia, USA
         type:PostalAddress
      name:University of Pennsylvania
      address:
         name:Department of Neurosurgery, University of Pennsylvania, Philadelphia, USA
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Drew Weissman
      affiliation:
            name:University of Pennsylvania
            address:
               name:Department of Medicine, University of Pennsylvania, Philadelphia, USA
               type:PostalAddress
            type:Organization
      email:[email protected]
      name:Norbert Pardi
      affiliation:
            name:University of Pennsylvania
            address:
               name:Department of Medicine, University of Pennsylvania, Philadelphia, USA
               type:PostalAddress
            type:Organization
      name:Hiro Muramatsu
      affiliation:
            name:University of Pennsylvania
            address:
               name:Department of Neurosurgery, University of Pennsylvania, Philadelphia, USA
               type:PostalAddress
            type:Organization
      name:Katalin Karikó
      affiliation:
            name:University of Pennsylvania
            address:
               name:Department of Neurosurgery, University of Pennsylvania, Philadelphia, USA
               type:PostalAddress
            type:Organization
PostalAddress:
      name:Department of Medicine, University of Pennsylvania, Philadelphia, USA
      name:Department of Medicine, University of Pennsylvania, Philadelphia, USA
      name:Department of Neurosurgery, University of Pennsylvania, Philadelphia, USA
      name:Department of Neurosurgery, University of Pennsylvania, Philadelphia, USA
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