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Production of CD4+ and CD8+ T Cell Hybridomas | SpringerLink
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T cell hybridomas are very useful tools to investigate antigen presenting cell (APC) function. They were developed based on the fusion technology that led to monoclonal antibody section. Antigen-specific primary T cells are generated and fused to an immortal thymoma...
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Keywords {🔍}
pubmed, article, google, scholar, cas, cell, antigen, hybridomas, processing, canaday, protocol, cells, chapter, immunol, privacy, cookies, content, information, publish, david, methods, presentation, exp, med, central, search, production, technology, access, class, download, springer, usd, function, analysis, personal, data, log, journal, research, october, book, primary, hybridoma, nature, white, usa, res, human, hladrb,
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mhc class ii-restricted month download article/chapter collagen-induced arthritis elicited hla-drb11501 risk association privacy choices/manage cookies antigen-specific hybridomas human antigen processing mhc-restricted t device instant download antigen-specific primary faculté de médecine european economic area fusion technology monoclonal antibody section immortal thymoma line engineered metabolic selection regulatory mechanisms intrinsic kappler jw mouse footpad injections woc-colburn chronic hcv infection va merit grant nih grant ai080313 author information authors editor information editors hla-transgenic mice conditions privacy policy hiv-infected individuals gogal rm jr ]thymidine incorporation assay human dendritic cells cell receptor expression journal finder publish cell hybridomas protocol canaday dh unfused thymoma cells accepting optional cookies université paris descartes cell fusion partner t-cell hybridomas main content log antigen processing peptide targets hla-drb10301 van endert protocol canaday protocol usd 49 check access ethics access antigen presentation
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ScholarlyArticle:
headline:Production of CD4+ and CD8+ T Cell Hybridomas
pageEnd:307
pageStart:297
image:https://media.springernature.com/w153/springer-static/cover/book/978-1-62703-218-6.jpg
genre:
Springer Protocols
isPartOf:
name:Antigen Processing
isbn:
978-1-62703-218-6
978-1-62703-217-9
type:Book
publisher:
name:Humana Press
logo:
url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
type:ImageObject
type:Organization
author:
name:David H. Canaday
affiliation:
name:Case Western Reserve University
address:
name:Division of Infectious Disease, Case Western Reserve University, Cleveland, USA
type:PostalAddress
type:Organization
email:[email protected]
type:Person
keywords:Antigen presentation, T cell hybridoma, Antigen processing, Macrophage, Dendritic cell
description:T cell hybridomas are very useful tools to investigate antigen presenting cell (APC) function. They were developed based on the fusion technology that led to monoclonal antibody section. Antigen-specific primary T cells are generated and fused to an immortal thymoma line. Unfused thymoma cells are eliminated by engineered metabolic selection. Antigen-specific hybridomas are identified and may be characterized in detail. Primary T cells are preferable for studies of the regulatory mechanisms intrinsic to T cells, but for study of antigen presentation T cell hybridomas have advantages over primary T cell clones, including their relative uniformity, stability over time, and ready availability in large numbers for extensive antigen presentation experiments.
datePublished:2013
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name:Humana Press
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name:Case Western Reserve University
address:
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Person:
name:David H. Canaday
affiliation:
name:Case Western Reserve University
address:
name:Division of Infectious Disease, Case Western Reserve University, Cleveland, USA
type:PostalAddress
type:Organization
email:[email protected]
PostalAddress:
name:Division of Infectious Disease, Case Western Reserve University, Cleveland, USA
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