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Title:
Fluorescence Measurement of Mitochondrial Membrane Potential Changes in Cultured Cells | SpringerLink
Description:
The mitochondrial membrane potential is the dominant component of the proton-motive force that is the potential term in the proton circuit linking electron transport to ATP synthesis and other energy-dependent mitochondrial processes. Cationic fluorescent probes have...
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Schema {🗺️}

ScholarlyArticle:
      headline:Fluorescence Measurement of Mitochondrial Membrane Potential Changes in Cultured Cells
      pageEnd:133
      pageStart:119
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-61779-382-0.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:Mitochondrial Bioenergetics
         isbn:
            978-1-61779-382-0
            978-1-61779-381-3
         type:Book
      publisher:
         name:Humana Press
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:David G. Nicholls
            affiliation:
                  name:Buck Institute for Research on Aging
                  address:
                     name:Buck Institute for Research on Aging, Novato, USA
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      keywords:Mitochondria, Membrane potential, Neuron, Glutamate, Proton-motive force, Proton electrochemical potential, Tetramethylrhodamine methyl ester, Rhodamine 123, JC-1
      description:The mitochondrial membrane potential is the dominant component of the proton-motive force that is the potential term in the proton circuit linking electron transport to ATP synthesis and other energy-dependent mitochondrial processes. Cationic fluorescent probes have been used for many years to detect gross qualitative changes in mitochondrial membrane potentials in intact cell culture. In this chapter, I describe how these fluorescence signals may be used to obtain a semiquantitative measure of changes in mitochondrial membrane potential.
      datePublished:2012
      isAccessibleForFree:
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Book:
      name:Mitochondrial Bioenergetics
      isbn:
         978-1-61779-382-0
         978-1-61779-381-3
Organization:
      name:Humana Press
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Buck Institute for Research on Aging
      address:
         name:Buck Institute for Research on Aging, Novato, USA
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:David G. Nicholls
      affiliation:
            name:Buck Institute for Research on Aging
            address:
               name:Buck Institute for Research on Aging, Novato, USA
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Buck Institute for Research on Aging, Novato, USA
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