We are analyzing https://link.springer.com/protocol/10.1007/978-1-61779-210-6_9.

Title:
Ex Utero Culture and Live Imaging of Mouse Embryos | SpringerLink
Description:
Mouse genetic approaches when combined with live imaging tools have the potential to revolutionize our current understanding of mammalian biology. The availability and improvement of a wide variety of fluorescent proteins have provided indispensable tools to...
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Schema {🗺️}

ScholarlyArticle:
      headline:Ex Utero Culture and Live Imaging of Mouse Embryos
      pageEnd:257
      pageStart:243
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-61779-210-6.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:Vertebrate Embryogenesis
         isbn:
            978-1-61779-210-6
            978-1-61779-209-0
         type:Book
      publisher:
         name:Humana Press
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Anna Piliszek
            affiliation:
                  name:Sloan-Kettering Institute
                  address:
                     name:Developmental Biology Program, Sloan-Kettering Institute, New York, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Gloria S. Kwon
            affiliation:
                  name:Sloan-Kettering Institute
                  address:
                     name:Developmental Biology Program, Sloan-Kettering Institute, New York, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Anna-Katerina Hadjantonakis
            affiliation:
                  name:Sloan-Kettering Institute
                  address:
                     name:Developmental Biology Program, Sloan-Kettering Institute, New York, USA
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      keywords:Mouse embryo, ex utero culture, live imaging, fluorescent protein, time lapse
      description:Mouse genetic approaches when combined with live imaging tools have the potential to revolutionize our current understanding of mammalian biology. The availability and improvement of a wide variety of fluorescent proteins have provided indispensable tools to visualize cells in living organisms. It is now possible to generate genetically modified mouse strains expressing fluorescent proteins in a tissue-specific manner. These reporter-expressing strains make it possible to image dynamic cell behaviors in the context of a living embryo. Since mouse embryos develop within the uterus, live imaging experiments require culture conditions that closely mimic those in vivo. Over the past few decades, significant advances have been made in developing conditions for culturing both pre- and postimplantation stage embryos. In this chapter, we will discuss methods for ex utero culture of preimplantation and postimplantation stage mouse embryos. In particular, we will describe protocols for collecting embryos at various stages, setting up culture conditions for imaging and using laser scanning confocal microscopy to visualize live processes in mouse embryos expressing fluorescent reporters.
      datePublished:2011
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      isbn:
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      name:Sloan-Kettering Institute
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            address:
               name:Developmental Biology Program, Sloan-Kettering Institute, New York, USA
               type:PostalAddress
            type:Organization
      name:Gloria S. Kwon
      affiliation:
            name:Sloan-Kettering Institute
            address:
               name:Developmental Biology Program, Sloan-Kettering Institute, New York, USA
               type:PostalAddress
            type:Organization
      name:Anna-Katerina Hadjantonakis
      affiliation:
            name:Sloan-Kettering Institute
            address:
               name:Developmental Biology Program, Sloan-Kettering Institute, New York, USA
               type:PostalAddress
            type:Organization
      email:[email protected]
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      name:Developmental Biology Program, Sloan-Kettering Institute, New York, USA
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      name:Developmental Biology Program, Sloan-Kettering Institute, New York, USA
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