We are analyzing https://link.springer.com/protocol/10.1007/978-1-61779-005-8_26.

Title:
The Dual Use of RNA Aptamer Sequences for Affinity Purification and Localization Studies of RNAs and RNA–Protein Complexes | SpringerLink
Description:
RNA affinity tags (aptamers) have emerged as useful tools for the isolation of RNAs and ribonucleoprotein complexes from cell extracts. The streptavidin binding RNA aptamer binds with high affinity and is quickly and cleanly eluted with biotin under mild conditions...
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Keywords {🔍}

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Topics {✒️}

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Schema {🗺️}

ScholarlyArticle:
      headline:The Dual Use of RNA Aptamer Sequences for Affinity Purification and Localization Studies of RNAs and RNA–Protein Complexes
      pageEnd:444
      pageStart:423
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-61779-005-8.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:RNA Detection and Visualization
         isbn:
            978-1-61779-005-8
            978-1-61779-004-1
         type:Book
      publisher:
         name:Humana Press
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Scott C. Walker
            affiliation:
                  name:University of Michigan
                  address:
                     name:Department of Biological Chemistry, University of Michigan, Ann Arbor, USA
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
            name:Paul D. Good
            affiliation:
            type:Person
            name:Theresa A. Gipson
            affiliation:
            type:Person
            name:David R. Engelke
            affiliation:
            type:Person
      keywords:RNA, RNP isolation, Aptamer, FISH, Ribonucleoprotein, Streptavidin
      description:RNA affinity tags (aptamers) have emerged as useful tools for the isolation of RNAs and ribonucleoprotein complexes from cell extracts. The streptavidin binding RNA aptamer binds with high affinity and is quickly and cleanly eluted with biotin under mild conditions that retain intact complexes. We describe the use of the streptavidin binding aptamer as a tool for purification and discuss strategies towards the design and production of tagged RNAs with a focus on structured target RNAs. The aptamer site can be further exploited as a unique region for the hybridization of oligonucleotide probes and localization by fluorescent in situ hybridization (FISH). The aptamer insertion will allow the localization of a population of RNA species (such as mutants) to be viewed specifically, while in the presence of the wild type RNA. We describe the production of labeled oligonucleotide probes and the preparation of yeast cells for the localization of RNAs by FISH.
      datePublished:2011
      isAccessibleForFree:
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         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
      context:https://schema.org
Book:
      name:RNA Detection and Visualization
      isbn:
         978-1-61779-005-8
         978-1-61779-004-1
Organization:
      name:Humana Press
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:University of Michigan
      address:
         name:Department of Biological Chemistry, University of Michigan, Ann Arbor, USA
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Scott C. Walker
      affiliation:
            name:University of Michigan
            address:
               name:Department of Biological Chemistry, University of Michigan, Ann Arbor, USA
               type:PostalAddress
            type:Organization
      email:[email protected]
      name:Paul D. Good
      affiliation:
      name:Theresa A. Gipson
      affiliation:
      name:David R. Engelke
      affiliation:
PostalAddress:
      name:Department of Biological Chemistry, University of Michigan, Ann Arbor, USA
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

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