We are analyzing https://link.springer.com/protocol/10.1007/978-1-60761-811-9_14.

Title:
Stable Overexpression of miRNAs in Bone Marrow-Derived Murine Mast Cells Using Lentiviral Expression Vectors | SpringerLink
Description:
MicroRNAs (miRNAs) constitute a class of molecules regulating gene expression in many different cell types, including cells of the mammalian immune system. Indeed, changes in miRNA expression patterns have been implicated in various physiological and pathological...
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Keywords {🔍}

article, google, scholar, pubmed, cas, cells, mast, micrornas, expression, monticelli, vectors, mirnas, bone, murine, lentiviral, gene, med, immunol, nature, privacy, cookies, content, information, publish, research, immune, system, protocol, nat, search, mayoral, silvia, access, rev, data, log, journal, stable, marrowderived, cell, mammalian, marrow, role, responses, bmmcs, stem, allergy, clin, science, sun,

Topics {✒️}

mediate long-term engraftment xbp1-responsive os-9 variants human cd34+ cells bone marrow privacy choices/manage cookies mast cells modulate protein synthesis induced murine hematopoietic system inflammatory mast cells ceresio foundation fellowship antigen-presenting cells mayoral & silvia monticelli mammalian immune system mast-cell responses murine mcs includes polyamine-dna polyplexes showed stable expression mammalian endoplasmic reticulum adaptive immune responses european economic area post-transcriptional regulators c-kit expression modifying expression patterns releasing proinflammatory cytokines squamous epithelial carcinogenesis c-kit ligand pre-doctoral fellowship san raffaele university author information authors editor information editors immune system ramon journal finder publish conditions privacy policy misfolded protein conformers mirna expression patterns hiv-1 pol sequences inactivating lentivirus vector tween lentiviral vectors lentiviral expression vectors accepting optional cookies nod/scid mice main content log mast cells check access ethics access van nimwegen vivo gene delivery stable overexpression including cells protocol mayoral

Schema {🗺️}

ScholarlyArticle:
      headline:Stable Overexpression of miRNAs in Bone Marrow-Derived Murine Mast Cells Using Lentiviral Expression Vectors
      pageEnd:214
      pageStart:205
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-60761-811-9.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:MicroRNAs and the Immune System
         isbn:
            978-1-60761-811-9
            978-1-60761-810-2
         type:Book
      publisher:
         name:Humana Press
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Ramon J. Mayoral
            affiliation:
                  name:Institute for Research in Biomedicine
                  address:
                     name:Institute for Research in Biomedicine, Bellinzona, Switzerland
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Silvia Monticelli
            affiliation:
                  name:Institute for Research in Biomedicine
                  address:
                     name:Institute for Research in Biomedicine, Bellinzona, Switzerland
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      keywords:Long Terminal Repeat, Toluidine Blue, Addgene Plasmid, TNES Buffer, Packaging Protein
      description:MicroRNAs (miRNAs) constitute a class of molecules regulating gene expression in many different cell types, including cells of the mammalian immune system. Indeed, changes in miRNA expression patterns have been implicated in various physiological and pathological processes. Mast cells (MCs) are hematopoietic cells that originate in the bone marrow and migrate into the tissues, where they mature and reside. They have an important immunoregulatory and effector role in IgE-associated allergic disorders, as well as in certain innate and adaptive immune responses. An effective way to explore the functions of miRNAs in murine MCs includes the modification of miRNA expression in primary bone marrow-derived mast cells (BMMCs), followed by the analysis of the phenotypic consequences of such perturbation. In this chapter, we describe how to differentiate BMMCs and transduce them with lentiviruses. As an example, we expressed miR-221 and miR-222, which showed stable expression in BMMCs and acted as post-transcriptional regulators of c-Kit expression.
      datePublished:2010
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Book:
      name:MicroRNAs and the Immune System
      isbn:
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         978-1-60761-810-2
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      name:Humana Press
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      name:Institute for Research in Biomedicine
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      affiliation:
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            address:
               name:Institute for Research in Biomedicine, Bellinzona, Switzerland
               type:PostalAddress
            type:Organization
      name:Silvia Monticelli
      affiliation:
            name:Institute for Research in Biomedicine
            address:
               name:Institute for Research in Biomedicine, Bellinzona, Switzerland
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Institute for Research in Biomedicine, Bellinzona, Switzerland
      name:Institute for Research in Biomedicine, Bellinzona, Switzerland
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