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Title:
Measurement of Mitochondrial ROS Production | SpringerLink
Description:
The significance of reactive oxygen species (ROS) as aggravating or primary factors in numerous pathologies is widely recognized, with mitochondria being considered the major intracellular source of ROS. It is not yet possible to routinely measure mitochondrial ROS...
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28 years and 1 months (reg. 1997-05-29).

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Questions {❓}

Giorgio M, Trinei M, Migliaccio E, Pelicci PG (2007) Hydrogen peroxide: a metabolic by-product or a common mediator of ageing signals?
ROS production by mitochondria: function or dysfunction?
Sawyer DT, Valentine JS (1981) How super is superoxide?

Schema {🗺️}

ScholarlyArticle:
      headline:Measurement of Mitochondrial ROS Production
      pageEnd:255
      pageStart:245
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-60761-756-3.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:Protein Misfolding and Cellular Stress in Disease and Aging
         isbn:
            978-1-60761-756-3
            978-1-60761-755-6
         type:Book
      publisher:
         name:Humana Press
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Anatoly A. Starkov
            affiliation:
                  name:Weill Medical College of Cornell University
                  address:
                     name:Department of Neurology and Neuroscience, Weill Medical College of Cornell University, New York, USA
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      keywords:Reactive oxygen species, Mitochondria, Amplex, Oxidative stress
      description:The significance of reactive oxygen species (ROS) as aggravating or primary factors in numerous pathologies is widely recognized, with mitochondria being considered the major intracellular source of ROS. It is not yet possible to routinely measure mitochondrial ROS in animals or cultured cells with a reasonable degree of certainty. However, at the level of isolated mitochondria, one can easily monitor and quantify the rate of ROS production, identify major sites of ROS production, and compare the rates of ROS production in mitochondria isolated from normal and diseased tissue. In this chapter, we describe in detail the most recent and reliable method to measure mitochondrial ROS as the rate of H2O2 emission. This method may be employed with minimal modifications to measure H2O2 production by mitochondria isolated from various tissues and under a wide variety of experimental conditions.
      datePublished:2010
      isAccessibleForFree:
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Book:
      name:Protein Misfolding and Cellular Stress in Disease and Aging
      isbn:
         978-1-60761-756-3
         978-1-60761-755-6
Organization:
      name:Humana Press
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Weill Medical College of Cornell University
      address:
         name:Department of Neurology and Neuroscience, Weill Medical College of Cornell University, New York, USA
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Anatoly A. Starkov
      affiliation:
            name:Weill Medical College of Cornell University
            address:
               name:Department of Neurology and Neuroscience, Weill Medical College of Cornell University, New York, USA
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Department of Neurology and Neuroscience, Weill Medical College of Cornell University, New York, USA
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