We are analyzing https://link.springer.com/protocol/10.1007/978-1-60761-753-2_15.

Title:
A Rapid and General Assay for Monitoring Endogenous Gene Modification | SpringerLink
Description:
The development of zinc finger nucleases for targeted gene modification can benefit from rapid functional assays that directly quantify activity at the endogenous target. Here we describe a simple procedure for quantifying mutations that result from DNA double-strand...
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28 years and 1 months (reg. 1997-05-29).

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Keywords {🔍}

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Topics {✒️}

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Schema {🗺️}

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      headline:A Rapid and General Assay for Monitoring Endogenous Gene Modification
      pageEnd:256
      pageStart:247
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      genre:
         Springer Protocols
      isPartOf:
         name:Engineered Zinc Finger Proteins
         isbn:
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            978-1-60761-752-5
         type:Book
      publisher:
         name:Humana Press
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      author:
            name:Dmitry Y. Guschin
            affiliation:
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      keywords:Zinc finger nuclease (ZFN), designed zinc finger proteins, non-homologous end joining (NHEJ), Surveyor nuclease, Cel 1, genome modification
      description:The development of zinc finger nucleases for targeted gene modification can benefit from rapid functional assays that directly quantify activity at the endogenous target. Here we describe a simple procedure for quantifying mutations that result from DNA double-strand break repair via non-homologous end joining. The assay is based on the ability of the Surveyor nuclease to selectively cleave distorted duplex DNA formed via cross-annealing of mutated and wild-type sequence.
      datePublished:2010
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