We are analyzing https://link.springer.com/protocol/10.1007/978-1-60761-411-1_4.

Title:
Identification of ROS Using Oxidized DCFDA and Flow-Cytometry | SpringerLink
Description:
Cells constantly generate reactive oxygen species (ROS) during aerobic metabolism. The ROS generation plays an important protective and functional role in the immune system. The cell is armed with a powerful antioxidant defense system to combat excessive production...
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Keywords {🔍}

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Topics {✒️}

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Questions {❓}

Halliwell B, Whitemann M (2004) Measuring reactive species and oxidative damage in vivo and in cell culture:how should you do it and do the results mean?

Schema {🗺️}

ScholarlyArticle:
      headline:Identification of ROS Using Oxidized DCFDA and Flow-Cytometry
      pageEnd:72
      pageStart:57
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-60761-411-1.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:Advanced Protocols in Oxidative Stress II
         isbn:
            978-1-60761-411-1
            978-1-60761-410-4
         type:Book
      publisher:
         name:Humana Press
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Evgeniy Eruslanov
            affiliation:
                  name:University of Florida
                  address:
                     name:Shands Cancer Center and Department of Urology, University of Florida, Gainesville, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Sergei Kusmartsev
            affiliation:
                  name:University of Florida
                  address:
                     name:Shands Cancer Center and Department of Urology, University of Florida, Gainesville, USA
                     type:PostalAddress
                  type:Organization
            type:Person
      keywords:Reactive oxygen species (ROS), Oxidative stress, 2′-7′-dichlorodihydrofluorescein diacetate (DCFH-DA), 2′-7′-dichlorofluorescein (DCF), Flow-cytometry, Immature myeloid cells (ImC)
      description:Cells constantly generate reactive oxygen species (ROS) during aerobic metabolism. The ROS generation plays an important protective and functional role in the immune system. The cell is armed with a powerful antioxidant defense system to combat excessive production of ROS. Oxidative stress occurs in cells when the generation of ROS overwhelms the cells’ natural antioxidant defenses. ROS and the oxidative damage are thought to play an important role in many human diseases including cancer, atherosclerosis, other neurodegenerative diseases and diabetes. Thus, establishing their precise role requires the ability to measure ROS accurately and the oxidative damage that they cause. There are many methods for measuring free radical production in cells. The most straightforward techniques use cell permeable fluorescent and chemiluminescent probes. 2′-7′-Dichlorodihydrofluorescein diacetate (DCFH-DA) is one of the most widely used techniques for directly measuring the redox state of a cell. It has several advantages over other techniques developed. It is very easy to use, extremely sensitive to changes in the redox state of a cell, inexpensive and can be used to follow changes in ROS over time.
      datePublished:2010
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Book:
      name:Advanced Protocols in Oxidative Stress II
      isbn:
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         978-1-60761-410-4
Organization:
      name:Humana Press
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         type:ImageObject
      name:University of Florida
      address:
         name:Shands Cancer Center and Department of Urology, University of Florida, Gainesville, USA
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      address:
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Person:
      name:Evgeniy Eruslanov
      affiliation:
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            address:
               name:Shands Cancer Center and Department of Urology, University of Florida, Gainesville, USA
               type:PostalAddress
            type:Organization
      name:Sergei Kusmartsev
      affiliation:
            name:University of Florida
            address:
               name:Shands Cancer Center and Department of Urology, University of Florida, Gainesville, USA
               type:PostalAddress
            type:Organization
PostalAddress:
      name:Shands Cancer Center and Department of Urology, University of Florida, Gainesville, USA
      name:Shands Cancer Center and Department of Urology, University of Florida, Gainesville, USA
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