We are analyzing https://link.springer.com/protocol/10.1007/978-1-60761-322-0_22.

Title:
Comprehensive Quantitative Analysis of Bioactive Sphingolipids by High-Performance Liquid Chromatography–Tandem Mass Spectrometry | SpringerLink
Description:
There has been a recent explosion in research concerning novel bioactive sphingolipids (SPLs) such as ceramide (Cer), sphingosine (Sph), and sphingosine 1-phosphate (Sph-1P) and this has necessitated the development of accurate and user-friendly methodology for...
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Topics {✒️}

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Questions {❓}

, (1998) Sphingomyelin synthase, a potential regulator of intracellular levels of ceramide and diacylglycerol during SV40 transformation – Does sphingomyelin synthase account for the putative, phosphatidylcholine-specific phospholipase C?

Schema {🗺️}

ScholarlyArticle:
      headline:Comprehensive Quantitative Analysis of Bioactive Sphingolipids by High-Performance Liquid Chromatography–Tandem Mass Spectrometry
      pageEnd:467
      pageStart:443
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-60761-322-0.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:Lipidomics
         isbn:
            978-1-60761-322-0
            978-1-60761-321-3
         type:Book
      publisher:
         name:Humana Press
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
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      author:
            name:Jacek Bielawski
            affiliation:
                  name:Medical University of South Carolina
                  address:
                     name:Department of Biochemistry and Molecular Biology, Lipidomic Core Mass Spectrometry Lab, Medical University of South Carolina, Charleston, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Jason S. Pierce
            affiliation:
                  name:Medical University of South Carolina
                  address:
                     name:Department of Biochemistry and Molecular Biology, Lipidomic Core Mass Spectrometry Lab, Medical University of South Carolina, Charleston, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Justin Snider
            affiliation:
                  name:Medical University of South Carolina
                  address:
                     name:Department of Biochemistry and Molecular Biology, Lipidomic Core Mass Spectrometry Lab, Medical University of South Carolina, Charleston, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Barbara Rembiesa
            affiliation:
                  name:Medical University of South Carolina
                  address:
                     name:Department of Biochemistry and Molecular Biology, Lipidomic Core Mass Spectrometry Lab, Medical University of South Carolina, Charleston, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Zdzislaw M. Szulc
            affiliation:
                  name:Medical University of South Carolina
                  address:
                     name:Department of Biochemistry and Molecular Biology, Lipidomic Core Mass Spectrometry Lab, Medical University of South Carolina, Charleston, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Alicja Bielawska
            affiliation:
                  name:Medical University of South Carolina
                  address:
                     name:Department of Biochemistry and Molecular Biology, Lipidomic Core Mass Spectrometry Lab, Medical University of South Carolina, Charleston, USA
                     type:PostalAddress
                  type:Organization
            type:Person
      keywords:Endogenous sphingolipid, Ceramide, Dihydroceramide, Sphingoid base, Sphingoid base phosphate, Sphingosine, Sphingosine 1-phosphate, Sphingomyelin, HPLC–MS analysis, ESI/MS/MS analysis
      description:There has been a recent explosion in research concerning novel bioactive sphingolipids (SPLs) such as ceramide (Cer), sphingosine (Sph), and sphingosine 1-phosphate (Sph-1P) and this has necessitated the development of accurate and user-friendly methodology for analyzing and quantitating the endogenous levels of these molecules. ESI/MS/MS methodology provides a universal tool used for detecting and monitoring changes in SPL levels and composition from biological materials. Simultaneous ESI/MS/MS analysis of sphingoid bases (SBs), sphingoid base 1-phosphates (SB-1Ps), ceramides (Cers), ceramide 1-phosphates (Cer-1P), glucosyl/galactosyl-ceramides (Glu-Cers), and sphingomyelins (SMs) is performed on a Thermo Fisher Scientific triple quadrupole mass spectrometer operating in a multiple reaction monitoring (MRM) positive ionization mode. Biological materials (cells, tissues, or physiological fluids) are fortified with internal standards (ISs), extracted into a one-phase neutral organic solvent system, and analyzed by a LC/MS/MS system. Qualitative analysis (identification) of SPLs is performed by a Parent Ion scan of a common fragment ion characteristic for a particular class of SPLs. Quantitative analysis is based on calibration curves generated by spiking an artificial matrix with known amounts of target analyte, synthetic standards, and an equal amount of IS. The calibration curves are constructed by plotting the peak area ratios of analyte to the respective IS against concentration, using a linear regression model. This robust analytical procedure can determine the composition of endogenous sphingolipids (ESPLs) in varied biological materials and achieve a detection limit of subpicomole level. This methodology constitutes a “Lipidomic” approach to study the SPLs metabolism, defining a function of distinct subspecies of individual bioactive SPL classes.
      datePublished:2009
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      name:Medical University of South Carolina
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         name:Department of Biochemistry and Molecular Biology, Lipidomic Core Mass Spectrometry Lab, Medical University of South Carolina, Charleston, USA
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      name:Jason S. Pierce
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      name:Barbara Rembiesa
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            address:
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               type:PostalAddress
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      name:Zdzislaw M. Szulc
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            name:Medical University of South Carolina
            address:
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               type:PostalAddress
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      name:Alicja Bielawska
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      name:Department of Biochemistry and Molecular Biology, Lipidomic Core Mass Spectrometry Lab, Medical University of South Carolina, Charleston, USA
      name:Department of Biochemistry and Molecular Biology, Lipidomic Core Mass Spectrometry Lab, Medical University of South Carolina, Charleston, USA
      name:Department of Biochemistry and Molecular Biology, Lipidomic Core Mass Spectrometry Lab, Medical University of South Carolina, Charleston, USA
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