We are analyzing https://link.springer.com/protocol/10.1007/978-1-60327-360-2_32.

Title:
Complexation of siRNA and pDNA with Cationic Liposomes: The Important Aspects in Lipoplex Preparation | SpringerLink
Description:
In the last two decades, cationic liposomes have been investigated as vehicles for nucleic acids [plasmid DNA (pDNA) and small interfering RNA (siRNA)] delivery in vitro and in vivo. The formation of cationic liposomes–nucleic acids complexes, termed...
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Keywords {🔍}

google, scholar, cationic, article, cas, pubmed, delivery, gene, liposomes, lipoplexes, research, sirna, kiwada, dna, privacy, cookies, content, information, publish, protocol, complexes, efficiency, transfection, chapter, search, pdna, lipoplex, barichello, ishida, rna, vivo, data, log, journal, preparation, mario, hiroshi, part, methods, nucleic, plasmid, formation, liposome, lipid, access, expression, cells, nature, biochim, biophys,

Topics {✒️}

springer science+business media cationic lipid-coated polyplexes cationic lipid-dna complexes cationic detergent/dna/liposomes tatsuhiro ishida & hiroshi kiwada privacy choices/manage cookies small interfering rna jose mario barichello humana press enhanced gene delivery journal finder publish cationic lipid nitrogen cationic lipid formulations delivered plasmid dna dotap-dope liposomes viral cationic vectors european economic area surface zeta potential post-transcription process differential intracellular distribution hirsch-lerner lipoplex preparation protocol conditions privacy policy cationic liposomal vector author information authors editor information editors advanced medical technology cultured mammalian cells live cos-7 cells nucleic acid delivery increased systemic delivery high transfection activity lasic dd selective gene delivery accepting optional cookies polymers mediated vectors templeton ns gene-silencing effect almofti mr main content log liposome complexes online protocol barichello rna interference gene-expression gene expression journal publish cationic lipids sullivan sm plasmid dna

Schema {🗺️}

ScholarlyArticle:
      headline:Complexation of siRNA and pDNA with Cationic Liposomes: The Important Aspects in Lipoplex Preparation
      pageEnd:472
      pageStart:461
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-60327-360-2.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:Liposomes
         isbn:
            978-1-60327-360-2
            978-1-60327-359-6
         type:Book
      publisher:
         name:Humana Press
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:José Mario Barichello
            affiliation:
                  name:The University of Tokushima
                  address:
                     name:Department of Pharmacokinetics and Biopharmaceutics, Subdivision of Biopharmaceutical Sciences, Institute of Health Biosciences, The University of Tokushima, Tokushima, Japan
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Tatsuhiro Ishida
            affiliation:
                  name:The University of Tokushima
                  address:
                     name:Department of Pharmacokinetics and Biopharmaceutics, Subdivision of Biopharmaceutical Sciences, Institute of Health Biosciences, The University of Tokushima, Tokushima, Japan
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Hiroshi Kiwada
            affiliation:
                  name:The University of Tokushima
                  address:
                     name:Department of Pharmacokinetics and Biopharmaceutics, Subdivision of Biopharmaceutical Sciences, Institute of Health Biosciences, The University of Tokushima, Tokushima, Japan
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      keywords:Cationic liposome, siRNA, pDNA, Complex formation, siRNA-lipoplex, pDNA-lipoplex, N/P ratio, Lipoplex formation under vortex-mixing, Spontaneous lipoplex formation
      description:In the last two decades, cationic liposomes have been investigated as vehicles for nucleic acids [plasmid DNA (pDNA) and small interfering RNA (siRNA)] delivery in vitro and in vivo. The formation of cationic liposomes–nucleic acids complexes, termed lipoplexes, depends on a number of experimental variables. The quality of the nucleic acid and the cationic liposome as well as the selection of diluents for diluting the concentrated stocks strongly affect the resulting lipoplexes and their efficiency of gene-expression or gene-silencing effect following transfection. In addition, the molar ratio of cationic lipid nitrogen (N) to siRNA or pDNA phosphate (P) (N/P ratio) influences the final characteristics of the lipoplexes, such as size, surface zeta potential, and reproducibility, thereby reflecting their efficiency following transfection. The methods presented in this chapter could be helpful to obtain reliable and reproducible lipoplexes and experimental results.
      datePublished:2010
      isAccessibleForFree:
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      context:https://schema.org
Book:
      name:Liposomes
      isbn:
         978-1-60327-360-2
         978-1-60327-359-6
Organization:
      name:Humana Press
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:The University of Tokushima
      address:
         name:Department of Pharmacokinetics and Biopharmaceutics, Subdivision of Biopharmaceutical Sciences, Institute of Health Biosciences, The University of Tokushima, Tokushima, Japan
         type:PostalAddress
      name:The University of Tokushima
      address:
         name:Department of Pharmacokinetics and Biopharmaceutics, Subdivision of Biopharmaceutical Sciences, Institute of Health Biosciences, The University of Tokushima, Tokushima, Japan
         type:PostalAddress
      name:The University of Tokushima
      address:
         name:Department of Pharmacokinetics and Biopharmaceutics, Subdivision of Biopharmaceutical Sciences, Institute of Health Biosciences, The University of Tokushima, Tokushima, Japan
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:José Mario Barichello
      affiliation:
            name:The University of Tokushima
            address:
               name:Department of Pharmacokinetics and Biopharmaceutics, Subdivision of Biopharmaceutical Sciences, Institute of Health Biosciences, The University of Tokushima, Tokushima, Japan
               type:PostalAddress
            type:Organization
      name:Tatsuhiro Ishida
      affiliation:
            name:The University of Tokushima
            address:
               name:Department of Pharmacokinetics and Biopharmaceutics, Subdivision of Biopharmaceutical Sciences, Institute of Health Biosciences, The University of Tokushima, Tokushima, Japan
               type:PostalAddress
            type:Organization
      name:Hiroshi Kiwada
      affiliation:
            name:The University of Tokushima
            address:
               name:Department of Pharmacokinetics and Biopharmaceutics, Subdivision of Biopharmaceutical Sciences, Institute of Health Biosciences, The University of Tokushima, Tokushima, Japan
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Department of Pharmacokinetics and Biopharmaceutics, Subdivision of Biopharmaceutical Sciences, Institute of Health Biosciences, The University of Tokushima, Tokushima, Japan
      name:Department of Pharmacokinetics and Biopharmaceutics, Subdivision of Biopharmaceutical Sciences, Institute of Health Biosciences, The University of Tokushima, Tokushima, Japan
      name:Department of Pharmacokinetics and Biopharmaceutics, Subdivision of Biopharmaceutical Sciences, Institute of Health Biosciences, The University of Tokushima, Tokushima, Japan
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