We are analyzing https://link.springer.com/protocol/10.1007/978-1-59745-060-7_18.

Title:
Bone Marrow-Derived Mesenchymal Stem Cells: Isolation, Expansion, Characterization, Viral Transduction, and Production of Conditioned Medium | SpringerLink
Description:
Mesenchymal stem cells (MSCs) are defined as self-renewing and multipotent cells capable of differentiating into multiple cell types, including osteocytes, chondrocytes, adipocytes, hepatocytes, myocytes, neurons, and cardiomyocytes. MSCs were originally isolated...
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Science
Education
Telecommunications

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 7,642,828 visitors per month in the current month.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We can't tell how the site generates income.

Many websites are intended to earn money, but some serve to share ideas or build connections. Websites exist for all kinds of purposes. This might be one of them. Link.springer.com might be making money, but it's not detectable how they're doing it.

Keywords {🔍}

cells, google, scholar, stem, pubmed, article, cas, mesenchymal, marrow, bone, cell, mscs, stromal, gnecchi, melo, vitro, human, privacy, cookies, content, information, publish, medicine, protocol, isolation, characterization, conditioned, methods, paracrine, med, prockop, protection, search, regenerative, expansion, viral, medium, molecular, tissue, chapter, mouse, access, physiol, nature, exp, res, caplan, pratt, dzau, zhang,

Topics {✒️}

fluorescence-activated cell sorting mouse bone marrow target paracrine/autocrine mediator improve cardiac function mesenchymal stem cells cell-based gene therapy springer science+business media marrow stromal cells myogenic cells derived protocol stem cells bone marrow stroma demonstrate site-specific differentiation haemopoietic stem cells multipotent cells capable neonatal mouse brains privacy choices/manage cookies spleen colonies derived google scholar colony-forming cells editor information editors multiple cell types regenerative medicine humana press cell-based therapies specific markers renders journal finder publish european economic area long-term engraftment cerebral ischemia model stem cells 25 stem cells concentrated conditioned medium infrequent cellular fusion viral vectors rendering conditions privacy policy paracrine action accounts accepting optional cookies check access adult rat acute kidney injury ethics access protocol cite main content log ischemic myocardium protocol gnecchi cell therapy precursors cell biochem 98 tissue repair protocols

Schema {🗺️}

ScholarlyArticle:
      headline:Bone Marrow-Derived Mesenchymal Stem Cells: Isolation, Expansion, Characterization, Viral Transduction, and Production of Conditioned Medium
      pageEnd:294
      pageStart:281
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-59745-060-7.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:Stem Cells in Regenerative Medicine
         isbn:
            978-1-59745-060-7
            978-1-58829-797-6
         type:Book
      publisher:
         name:Humana Press
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Massimiliano Gnecchi
            affiliation:
                  name:Fondazione IRCCS Policlinico San Matteo and University of Pavia
                  address:
                     name:Department of Cardiology, Fondazione IRCCS Policlinico San Matteo and University of Pavia, Italy
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Luis G. Melo
            affiliation:
                  name:Queen’s University
                  address:
                     name:Department of Physiology, Queen’s University, Kingston, Canada
                     type:PostalAddress
                  type:Organization
            type:Person
      keywords:Mesenchymal stem cells, cell surface markers, flow cytometry, gene transfer, conditioned medium, soluble factors, paracrine effect, drug discovery
      description:Mesenchymal stem cells (MSCs) are defined as self-renewing and multipotent cells capable of differentiating into multiple cell types, including osteocytes, chondrocytes, adipocytes, hepatocytes, myocytes, neurons, and cardiomyocytes. MSCs were originally isolated from the bone marrow stroma but they have recently been identified also in other tissues, such as fat, epidermis, and cord blood. Several methods have been used for MSC isolation. The most common method is based on the ability of the MSCs to selectively adhere to plastic surfaces. Phenotypic characterization of MSCs is usually carried out using immunocytochemical detection or fluorescence-activated cell sorting (FACS) analysis of cell surface molecule expression. However, the lack of specific markers renders the characterization of MSCs difficult and sometimes ambiguous. MSCs posses remarkable expansion potential in culture and are highly amenable to genetic modification with various viral vectors rendering them optimal vehicles for cell-based gene therapy. Most importantly, MSC plasticity and the possibility to use them as autologous cells render MSCs suitable for cell therapy and tissue engineering. Furthermore, it is known that MSCs produce and secrete a great variety of cytokines and chemokines that play beneficial paracrine actions when MSCs are used for tissue repair. In this chapter, we describe methods for isolation, ex vivo expansion, phenotypic characterization, and viral infection of MSCs from mouse bone marrow. We also describe a method for preparation of conditioned and concentrated conditioned medium from MSCs. The conditioned medium can be easily tested both in vitro and in vivo when a particular paracrine effect (i.e., cytoprotection) is hypothesized to be an important mechanism of action of the MSCs and/or screened to identify a target paracrine/autocrine mediator.
      datePublished:2009
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
      context:https://schema.org
Book:
      name:Stem Cells in Regenerative Medicine
      isbn:
         978-1-59745-060-7
         978-1-58829-797-6
Organization:
      name:Humana Press
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Fondazione IRCCS Policlinico San Matteo and University of Pavia
      address:
         name:Department of Cardiology, Fondazione IRCCS Policlinico San Matteo and University of Pavia, Italy
         type:PostalAddress
      name:Queen’s University
      address:
         name:Department of Physiology, Queen’s University, Kingston, Canada
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Massimiliano Gnecchi
      affiliation:
            name:Fondazione IRCCS Policlinico San Matteo and University of Pavia
            address:
               name:Department of Cardiology, Fondazione IRCCS Policlinico San Matteo and University of Pavia, Italy
               type:PostalAddress
            type:Organization
      name:Luis G. Melo
      affiliation:
            name:Queen’s University
            address:
               name:Department of Physiology, Queen’s University, Kingston, Canada
               type:PostalAddress
            type:Organization
PostalAddress:
      name:Department of Cardiology, Fondazione IRCCS Policlinico San Matteo and University of Pavia, Italy
      name:Department of Physiology, Queen’s University, Kingston, Canada
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

External Links {🔗}(114)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js

CDN Services {📦}

Pbgrd

4s.

LINK . SPRINGER . COM {}