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Title:
Morphological Identification of Melanopsin-Expressing Retinal Ganglion Cell Subtypes in Mice | SpringerLink
Description:
Melanopsin-expressing, intrinsically photosensitive retinal ganglion cells (ipRGCs) are a relatively recently discovered class of photoreceptor. ipRGCs can be subdivided into at least five subtypes (M1-M5), each of which has a distinct complement of morphological and...
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Keywords {🔍}

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Topics {✒️}

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Schema {🗺️}

ScholarlyArticle:
      headline:Morphological Identification of Melanopsin-Expressing Retinal Ganglion Cell Subtypes in Mice
      pageEnd:287
      pageStart:275
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-4939-7720-8.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:Mouse Retinal Phenotyping
         isbn:
            978-1-4939-7720-8
            978-1-4939-7719-2
         type:Book
      publisher:
         name:Springer New York
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Seul Ki Lee
            affiliation:
                  name:Northwestern University
                  address:
                     name:Department of Neurobiology, Northwestern University, Evanston, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Tiffany M. Schmidt
            affiliation:
                  name:Northwestern University
                  address:
                     name:Department of Neurobiology, Northwestern University, Evanston, USA
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      keywords:Melanopsin, Subtypes, Patch clamp electrophysiology, Dendritic stratification, SMI-32, Retina, Opn4 , Mouse, Intrinsically photosensitive retinal ganglion cell
      description:Melanopsin-expressing, intrinsically photosensitive retinal ganglion cells (ipRGCs) are a relatively recently discovered class of photoreceptor. ipRGCs can be subdivided into at least five subtypes (M1-M5), each of which has a distinct complement of morphological and physiological properties. ipRGC subtypes can be identified morphologically based on a combination of dendritic morphology and immunostaining for a cell-type specific marker. In this chapter, we describe methods for conclusively identifying each of the five ipRGC subtypes through a combination of patch clamp electrophysiology, Neurobiotin filling, visualization of ipRGC dendrites, and immunostaining for the marker SMI-32.
      datePublished:2018
      isAccessibleForFree:
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Book:
      name:Mouse Retinal Phenotyping
      isbn:
         978-1-4939-7720-8
         978-1-4939-7719-2
Organization:
      name:Springer New York
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Northwestern University
      address:
         name:Department of Neurobiology, Northwestern University, Evanston, USA
         type:PostalAddress
      name:Northwestern University
      address:
         name:Department of Neurobiology, Northwestern University, Evanston, USA
         type:PostalAddress
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Person:
      name:Seul Ki Lee
      affiliation:
            name:Northwestern University
            address:
               name:Department of Neurobiology, Northwestern University, Evanston, USA
               type:PostalAddress
            type:Organization
      name:Tiffany M. Schmidt
      affiliation:
            name:Northwestern University
            address:
               name:Department of Neurobiology, Northwestern University, Evanston, USA
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Department of Neurobiology, Northwestern University, Evanston, USA
      name:Department of Neurobiology, Northwestern University, Evanston, USA
WebPageElement:
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