We are analyzing https://link.springer.com/protocol/10.1007/978-1-4939-6703-2_11.

Title:
Fluorescence In Situ Hybridization on Tissue Sections | SpringerLink
Description:
Formalin-fixed paraffin-embedded (FFPE) tissues are typically the specimens available for FISH analysis of solid tissues, particularly of tumor specimens. Occasionally, tissue cores constructed as tissue microarrays from several patients are presented for...
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Education
Technology & Computing
Telecommunications

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
However, some sources were not loaded, we suggest to reload the page to get complete results.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We're unsure how the site profits.

Not every website is profit-driven; some are created to spread information or serve as an online presence. Websites can be made for many reasons. This could be one of them. Link.springer.com might be plotting its profit, but the way they're doing it isn't detectable yet.

Keywords {🔍}

protocol, hybridization, tissue, situ, lim, cancer, chapter, privacy, cookies, content, information, publish, fluorescence, google, scholar, springer, search, cytogenetics, sections, methods, formalinfixed, paraffinembedded, ffpe, clinical, american, download, usd, analysis, personal, data, log, journal, research, alvin, book, molecular, tissues, fish, assay, access, pubmed, article, eds, author, singapore, ebook, optional, media, parties, policy,

Topics {✒️}

paraffin-embedded tissue sections month download article/chapter lim & tse hui lim paraffin-embedded tissue target complementary sequences privacy choices/manage cookies device instant download clinical oncology/college editor information editors european economic area include slide deparaffinization formalin-fixed dako north america tissue sections protocol situ hybridization techniques journal finder publish conditions privacy policy tissue cores constructed cell blocks derived genes/gene loci earlier ffpe preparations accepting optional cookies cancer cytogenetics hong kong prince ffpe tissue slides main content log protocols utilized social media permissions reprints rapid assay author correspondence protocol usd 49 protocol lim singapore general hospital solid tissues chapter log humana press protocol cite breast cancer situ hybridization journal publish privacy policy check access ethics access american society wan rights books a tissue sections fish analysis optional cookies

Schema {🗺️}

ScholarlyArticle:
      headline:Fluorescence In Situ Hybridization on Tissue Sections
      pageEnd:125
      pageStart:119
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-4939-6703-2.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:Cancer Cytogenetics
         isbn:
            978-1-4939-6703-2
            978-1-4939-6701-8
         type:Book
      publisher:
         name:Springer New York
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Alvin S. T. Lim
            affiliation:
                  name:Singapore General Hospital
                  address:
                     name:Cytogenetics Laboratory, Department of Molecular Pathology, Singapore General Hospital, Singapore, Singapore
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
            name:Tse Hui Lim
            affiliation:
                  name:Singapore General Hospital
                  address:
                     name:Cytogenetics Laboratory, Department of Molecular Pathology, Singapore General Hospital, Singapore, Singapore
                     type:PostalAddress
                  type:Organization
            type:Person
      keywords:Formalin-fixed paraffin-embedded tissues, Fluorescence in situ hybridization, Probes
      description:Formalin-fixed paraffin-embedded (FFPE) tissues are typically the specimens available for FISH analysis of solid tissues, particularly of tumor specimens. Occasionally, tissue cores constructed as tissue microarrays from several patients are presented for simultaneous evaluation. FFPE sections can also be prepared from cell blocks derived from cell suspensions. The interphase fluorescence in situ hybridization assay employs specific nucleic acid sequences (probes) that target complementary sequences of interest to detect gains or losses of genes/gene loci or a fusion gene within the tissue. In this chapter, we describe the protocols utilized in our laboratory and include slide deparaffinization, pretreatment, protease treatment, hybridization, washing, and counterstaining. This protocol can be applied to all of the earlier FFPE preparations. In general, the assay takes 3 consecutive days to complete, although a more rapid assay can be performed.
      datePublished:2017
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
      context:https://schema.org
Book:
      name:Cancer Cytogenetics
      isbn:
         978-1-4939-6703-2
         978-1-4939-6701-8
Organization:
      name:Springer New York
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Singapore General Hospital
      address:
         name:Cytogenetics Laboratory, Department of Molecular Pathology, Singapore General Hospital, Singapore, Singapore
         type:PostalAddress
      name:Singapore General Hospital
      address:
         name:Cytogenetics Laboratory, Department of Molecular Pathology, Singapore General Hospital, Singapore, Singapore
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Alvin S. T. Lim
      affiliation:
            name:Singapore General Hospital
            address:
               name:Cytogenetics Laboratory, Department of Molecular Pathology, Singapore General Hospital, Singapore, Singapore
               type:PostalAddress
            type:Organization
      email:[email protected]
      name:Tse Hui Lim
      affiliation:
            name:Singapore General Hospital
            address:
               name:Cytogenetics Laboratory, Department of Molecular Pathology, Singapore General Hospital, Singapore, Singapore
               type:PostalAddress
            type:Organization
PostalAddress:
      name:Cytogenetics Laboratory, Department of Molecular Pathology, Singapore General Hospital, Singapore, Singapore
      name:Cytogenetics Laboratory, Department of Molecular Pathology, Singapore General Hospital, Singapore, Singapore
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

External Links {🔗}(42)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js

CDN Services {📦}

Pbgrd

5.24s.

LINK . SPRINGER . COM {}