We are analyzing https://link.springer.com/protocol/10.1007/978-1-4939-6685-1_29.

Title:
Characterization, Quantification, and Visualization of Neutrophil Extracellular Traps | SpringerLink
Description:
Following the discovery of neutrophil extracellular traps (NETs) in 2004 by Brinkmann and colleagues, there has been extensive research into the role of NETs in a number of inflammatory diseases, including periodontitis. This chapter describes the current methods for...
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Schema {🗺️}

ScholarlyArticle:
      headline:Characterization, Quantification, and Visualization of Neutrophil Extracellular Traps
      pageEnd:497
      pageStart:481
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-4939-6685-1.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:Oral Biology
         isbn:
            978-1-4939-6685-1
            978-1-4939-6683-7
         type:Book
      publisher:
         name:Springer New York
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Phillipa C. White
            affiliation:
                  name:University of Birmingham
                  address:
                     name:Institute of Clinical Sciences, College of Medical and Dental Sciences, The School of Dentistry, University of Birmingham, Birmingham, UK
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Ilaria J. Chicca
            affiliation:
                  name:University of Birmingham
                  address:
                     name:Institute of Clinical Sciences, College of Medical and Dental Sciences, The School of Dentistry, University of Birmingham, Birmingham, UK
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Martin R. Ling
            affiliation:
                  name:University of Birmingham
                  address:
                     name:Institute of Clinical Sciences, College of Medical and Dental Sciences, The School of Dentistry, University of Birmingham, Birmingham, UK
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Helen J. Wright
            affiliation:
                  name:University of Birmingham
                  address:
                     name:Institute of Clinical Sciences, College of Medical and Dental Sciences, The School of Dentistry, University of Birmingham, Birmingham, UK
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Paul R. Cooper
            affiliation:
                  name:University of Birmingham
                  address:
                     name:Institute of Clinical Sciences, College of Medical and Dental Sciences, The School of Dentistry, University of Birmingham, Birmingham, UK
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Mike R. Milward
            affiliation:
                  name:University of Birmingham
                  address:
                     name:Institute of Clinical Sciences, College of Medical and Dental Sciences, The School of Dentistry, University of Birmingham, Birmingham, UK
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Iain L. C. Chapple
            affiliation:
                  name:University of Birmingham
                  address:
                     name:Institute of Clinical Sciences, College of Medical and Dental Sciences, The School of Dentistry, University of Birmingham, Birmingham, UK
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      keywords:Neutrophil extracellular traps, Reactive oxygen species, Fluorescence microscopy, SEM, Chemiluminescence, DNA, Elastase, Myeloperoxidase, Cathepsin G, Immunostaining
      description:Following the discovery of neutrophil extracellular traps (NETs) in 2004 by Brinkmann and colleagues, there has been extensive research into the role of NETs in a number of inflammatory diseases, including periodontitis. This chapter describes the current methods for the isolation of peripheral blood neutrophils for subsequent NET experiments, including approaches to quantify and visualize NET production, the ability of NETs to entrap and kill bacteria, and the removal of NETs by nuclease-containing plasma.
      datePublished:2017
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Book:
      name:Oral Biology
      isbn:
         978-1-4939-6685-1
         978-1-4939-6683-7
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      name:Springer New York
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         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
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      name:University of Birmingham
      address:
         name:Institute of Clinical Sciences, College of Medical and Dental Sciences, The School of Dentistry, University of Birmingham, Birmingham, UK
         type:PostalAddress
      name:University of Birmingham
      address:
         name:Institute of Clinical Sciences, College of Medical and Dental Sciences, The School of Dentistry, University of Birmingham, Birmingham, UK
         type:PostalAddress
      name:University of Birmingham
      address:
         name:Institute of Clinical Sciences, College of Medical and Dental Sciences, The School of Dentistry, University of Birmingham, Birmingham, UK
         type:PostalAddress
      name:University of Birmingham
      address:
         name:Institute of Clinical Sciences, College of Medical and Dental Sciences, The School of Dentistry, University of Birmingham, Birmingham, UK
         type:PostalAddress
      name:University of Birmingham
      address:
         name:Institute of Clinical Sciences, College of Medical and Dental Sciences, The School of Dentistry, University of Birmingham, Birmingham, UK
         type:PostalAddress
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      address:
         name:Institute of Clinical Sciences, College of Medical and Dental Sciences, The School of Dentistry, University of Birmingham, Birmingham, UK
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         name:Institute of Clinical Sciences, College of Medical and Dental Sciences, The School of Dentistry, University of Birmingham, Birmingham, UK
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Person:
      name:Phillipa C. White
      affiliation:
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            address:
               name:Institute of Clinical Sciences, College of Medical and Dental Sciences, The School of Dentistry, University of Birmingham, Birmingham, UK
               type:PostalAddress
            type:Organization
      name:Ilaria J. Chicca
      affiliation:
            name:University of Birmingham
            address:
               name:Institute of Clinical Sciences, College of Medical and Dental Sciences, The School of Dentistry, University of Birmingham, Birmingham, UK
               type:PostalAddress
            type:Organization
      name:Martin R. Ling
      affiliation:
            name:University of Birmingham
            address:
               name:Institute of Clinical Sciences, College of Medical and Dental Sciences, The School of Dentistry, University of Birmingham, Birmingham, UK
               type:PostalAddress
            type:Organization
      name:Helen J. Wright
      affiliation:
            name:University of Birmingham
            address:
               name:Institute of Clinical Sciences, College of Medical and Dental Sciences, The School of Dentistry, University of Birmingham, Birmingham, UK
               type:PostalAddress
            type:Organization
      name:Paul R. Cooper
      affiliation:
            name:University of Birmingham
            address:
               name:Institute of Clinical Sciences, College of Medical and Dental Sciences, The School of Dentistry, University of Birmingham, Birmingham, UK
               type:PostalAddress
            type:Organization
      name:Mike R. Milward
      affiliation:
            name:University of Birmingham
            address:
               name:Institute of Clinical Sciences, College of Medical and Dental Sciences, The School of Dentistry, University of Birmingham, Birmingham, UK
               type:PostalAddress
            type:Organization
      name:Iain L. C. Chapple
      affiliation:
            name:University of Birmingham
            address:
               name:Institute of Clinical Sciences, College of Medical and Dental Sciences, The School of Dentistry, University of Birmingham, Birmingham, UK
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Institute of Clinical Sciences, College of Medical and Dental Sciences, The School of Dentistry, University of Birmingham, Birmingham, UK
      name:Institute of Clinical Sciences, College of Medical and Dental Sciences, The School of Dentistry, University of Birmingham, Birmingham, UK
      name:Institute of Clinical Sciences, College of Medical and Dental Sciences, The School of Dentistry, University of Birmingham, Birmingham, UK
      name:Institute of Clinical Sciences, College of Medical and Dental Sciences, The School of Dentistry, University of Birmingham, Birmingham, UK
      name:Institute of Clinical Sciences, College of Medical and Dental Sciences, The School of Dentistry, University of Birmingham, Birmingham, UK
      name:Institute of Clinical Sciences, College of Medical and Dental Sciences, The School of Dentistry, University of Birmingham, Birmingham, UK
      name:Institute of Clinical Sciences, College of Medical and Dental Sciences, The School of Dentistry, University of Birmingham, Birmingham, UK
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