We are analyzing https://link.springer.com/protocol/10.1007/978-1-4939-6670-7_14.

Title:
Detection of Senescent Cells by Extracellular Markers Using a Flow Cytometry-Based Approach | SpringerLink
Description:
Senescence is a cellular process that is thought to have prognostic and therapeutic relevance in conditions such as cancer, aging, and fibrosis. However, current protocols for identifying senescent cells in vitro and in vivo have several drawbacks. Most markers used...
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Education
Science
Telecommunications

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
However, some sources were not loaded, we suggest to reload the page to get complete results.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We can't tell how the site generates income.

The purpose of some websites isn't monetary gain; they're meant to inform, educate, or foster collaboration. Everyone has unique reasons for building websites. This could be an example. Link.springer.com could have a money-making trick up its sleeve, but it's undetectable for now.

Keywords {🔍}

pubmed, article, google, scholar, cas, senescence, cells, senescent, cancer, cell, central, protocol, markers, macip, althubiti, aging, nature, human, privacy, cookies, content, information, publish, biology, cellular, serrano, leicester, research, search, oncogeneinduced, detection, flow, mohammad, salvador, access, tumor, download, university, york, springer, usd, personal, data, log, journal, book, molecular, protocols, chapter, nat,

Topics {✒️}

month download article/chapter flow cytometry-based approach customizable facs-based protocol rescue p21-induced senescence age-related chronic diseases oncogene-induced senescence markers p53-dependent icam-1 overexpression wild-type p53 triggers endogenous beta-galactosidase activity p21-mediated ros accumulation oncogene-induced senescence umm al-qura university growth arrest induced salvador macip human tumor cells privacy choices/manage cookies device instant download protocol althubiti require lengthy protocols beta-galactosidase activity identifying senescent cells detect senescent cells continuously nonproliferating cells european economic area classical staining techniques van de sluis senescent secretory phenotype author information authors editor information editors rapid senescence program journal finder publish glial cell senescence van deursen jm accepting optional cookies controlling cellular senescence lack sufficient specificity offer objective quantification protocol usd 49 conditions privacy policy main content log cell stress biology protocol cite current protocols senescent cells social media permissions reprints check access ethics access cancer res 73 humana press

Questions {❓}

Chinta SJ, Lieu CA, Demaria M, Laberge RM, Campisi J, Andersen JK (2013) Environmental stress, ageing and glial cell senescence: a novel mechanistic link to Parkinson's disease?

Schema {🗺️}

ScholarlyArticle:
      headline:Detection of Senescent Cells by Extracellular Markers Using a Flow Cytometry-Based Approach
      pageEnd:153
      pageStart:147
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-4939-6670-7.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:Oncogene-Induced Senescence
         isbn:
            978-1-4939-6670-7
            978-1-4939-6668-4
         type:Book
      publisher:
         name:Springer New York
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Mohammad Althubiti
            affiliation:
                  name:University of Leicester
                  address:
                     name:Mechanisms of Cancer and Aging Laboratory, Department of Molecular and Cell Biology, University of Leicester, LE1 7RH, Leicester, UK
                     type:PostalAddress
                  type:Organization
                  name:Cancer Research UK Leicester Centre
                  address:
                     name:Cancer Research UK Leicester Centre, Leicester, UK
                     type:PostalAddress
                  type:Organization
                  name:Umm Al-Qura University
                  address:
                     name:Department of Biochemistry, Faculty of Medicine, Umm Al-Qura University, Mecca, Saudi Arabia
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Salvador Macip
            affiliation:
                  name:University of Leicester
                  address:
                     name:Mechanisms of Cancer and Aging Laboratory, Department of Molecular and Cell Biology, University of Leicester, LE1 7RH, Leicester, UK
                     type:PostalAddress
                  type:Organization
                  name:Cancer Research UK Leicester Centre
                  address:
                     name:Cancer Research UK Leicester Centre, Leicester, UK
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      keywords:Senescence, Extracellular markers, Antibodies, Flow cytometry, SA-β-Gal
      description:Senescence is a cellular process that is thought to have prognostic and therapeutic relevance in conditions such as cancer, aging, and fibrosis. However, current protocols for identifying senescent cells in vitro and in vivo have several drawbacks. Most markers used lack sufficient specificity and false positives and negatives in common. In addition, classical staining techniques often require lengthy protocols and do not offer objective quantification. Recently, several novel markers of senescence associated with the plasma membrane have been identified. Here, we propose to take advantage of these markers to define a customizable FACS-based protocol to detect senescent cells using antibodies tagged with fluorescence dyes. This method has the advantage of being fast and allowing quantitation. Furthermore, its specificity is increased using several markers simultaneously.
      datePublished:2017
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
      context:https://schema.org
Book:
      name:Oncogene-Induced Senescence
      isbn:
         978-1-4939-6670-7
         978-1-4939-6668-4
Organization:
      name:Springer New York
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:University of Leicester
      address:
         name:Mechanisms of Cancer and Aging Laboratory, Department of Molecular and Cell Biology, University of Leicester, LE1 7RH, Leicester, UK
         type:PostalAddress
      name:Cancer Research UK Leicester Centre
      address:
         name:Cancer Research UK Leicester Centre, Leicester, UK
         type:PostalAddress
      name:Umm Al-Qura University
      address:
         name:Department of Biochemistry, Faculty of Medicine, Umm Al-Qura University, Mecca, Saudi Arabia
         type:PostalAddress
      name:University of Leicester
      address:
         name:Mechanisms of Cancer and Aging Laboratory, Department of Molecular and Cell Biology, University of Leicester, LE1 7RH, Leicester, UK
         type:PostalAddress
      name:Cancer Research UK Leicester Centre
      address:
         name:Cancer Research UK Leicester Centre, Leicester, UK
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Mohammad Althubiti
      affiliation:
            name:University of Leicester
            address:
               name:Mechanisms of Cancer and Aging Laboratory, Department of Molecular and Cell Biology, University of Leicester, LE1 7RH, Leicester, UK
               type:PostalAddress
            type:Organization
            name:Cancer Research UK Leicester Centre
            address:
               name:Cancer Research UK Leicester Centre, Leicester, UK
               type:PostalAddress
            type:Organization
            name:Umm Al-Qura University
            address:
               name:Department of Biochemistry, Faculty of Medicine, Umm Al-Qura University, Mecca, Saudi Arabia
               type:PostalAddress
            type:Organization
      name:Salvador Macip
      affiliation:
            name:University of Leicester
            address:
               name:Mechanisms of Cancer and Aging Laboratory, Department of Molecular and Cell Biology, University of Leicester, LE1 7RH, Leicester, UK
               type:PostalAddress
            type:Organization
            name:Cancer Research UK Leicester Centre
            address:
               name:Cancer Research UK Leicester Centre, Leicester, UK
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Mechanisms of Cancer and Aging Laboratory, Department of Molecular and Cell Biology, University of Leicester, LE1 7RH, Leicester, UK
      name:Cancer Research UK Leicester Centre, Leicester, UK
      name:Department of Biochemistry, Faculty of Medicine, Umm Al-Qura University, Mecca, Saudi Arabia
      name:Mechanisms of Cancer and Aging Laboratory, Department of Molecular and Cell Biology, University of Leicester, LE1 7RH, Leicester, UK
      name:Cancer Research UK Leicester Centre, Leicester, UK
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

External Links {🔗}(108)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js

CDN Services {📦}

Pbgrd

4.86s.

LINK . SPRINGER . COM {}