We are analyzing https://link.springer.com/protocol/10.1007/978-1-4939-6563-2_7.

Title:
Assessing Anti-miR Pharmacology with miRNA Polysome Shift Assay | SpringerLink
Description:
Target engagement measurements are critical for evaluating developmental drug candidates and their pharmacological activity. microRNA (miRNA) Polysome Shift Assay enables measurement of anti-miR drug target engagement (i.e. extent of miRNA inhibition) without the...
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Education
Careers
Technology & Computing

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 8,352,060 visitors per month in the current month.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We find it hard to spot revenue streams.

The purpose of some websites isn't monetary gain; they're meant to inform, educate, or foster collaboration. Everyone has unique reasons for building websites. This could be an example. Link.springer.com might be plotting its profit, but the way they're doing it isn't detectable yet.

Keywords {🔍}

mirna, article, pubmed, google, scholar, protocol, polysome, cas, drug, target, antimir, shift, assay, androsavich, privacy, cookies, content, information, publish, data, search, john, microrna, inhibition, chapter, download, author, springer, usd, personal, log, journal, research, assessing, book, biology, activity, access, central, micrornas, nat, nsmb, nature, york, ebook, function, optional, analysis, media, parties,

Topics {✒️}

assessing anti-mir pharmacology assessing anti-mir activity month download article/chapter drug target mirna single anti-mir anti-mirna drugs polysome shift assay guiding drug optimization privacy choices/manage cookies device instant download european economic area describes sample preparation healthy mouse liver translating messenger rnas genome-wide scale editor information editors journal finder publish conditions privacy policy author information authors accepting optional cookies actively translating polyribosomes protocol usd 49 protocol androsavich target tissues main content log protocol cite mrna targets social media nsmb1174 [pii] 10 nsmb1173 [pii] 10 permissions reprints therapeutic mirna pre-identify polysome fractionation humana press mirna inhibition journal publish privacy policy check access ethics access personal data direct measurement schmidt rights books a nat protoc 3 data protection androsavich part optional cookies manage preferences online

Schema {🗺️}

ScholarlyArticle:
      headline:Assessing Anti-miR Pharmacology with miRNA Polysome Shift Assay
      pageEnd:113
      pageStart:103
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-4939-6563-2.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:Drug Target miRNA
         isbn:
            978-1-4939-6563-2
            978-1-4939-6561-8
         type:Book
      publisher:
         name:Springer New York
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:John R. Androsavich
            affiliation:
                  name:Regulus Therapeutics, Inc.
                  address:
                     name:Regulus Therapeutics, Inc., San Diego, USA
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      keywords:microRNAs, Polysomes, Anti-miR oligonucleotides, microRNAs/antagonists and inhibitors, Pharmacology
      description:Target engagement measurements are critical for evaluating developmental drug candidates and their pharmacological activity. microRNA (miRNA) Polysome Shift Assay enables measurement of anti-miR drug target engagement (i.e. extent of miRNA inhibition) without the need to pre-identify or pre-validate downstream miRNA-regulated genes. This makes it useful for assessing anti-miR activity in target tissues or cells where biology of the inhibited miRNAs may not be well understood. In addition, miRNA Polysome Shift Assay can be multiplexed to assess inhibition of multiple miRNAs by a single anti-miR, thus guiding drug optimization for enhancing or avoiding these activities as desired. This chapter outlines the miRNA Polysome Shift Assay technique, describes sample preparation and quality control, and how to calculate and interpret results.
      datePublished:2017
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
      context:https://schema.org
Book:
      name:Drug Target miRNA
      isbn:
         978-1-4939-6563-2
         978-1-4939-6561-8
Organization:
      name:Springer New York
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Regulus Therapeutics, Inc.
      address:
         name:Regulus Therapeutics, Inc., San Diego, USA
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:John R. Androsavich
      affiliation:
            name:Regulus Therapeutics, Inc.
            address:
               name:Regulus Therapeutics, Inc., San Diego, USA
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Regulus Therapeutics, Inc., San Diego, USA
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

External Links {🔗}(64)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js

CDN Services {📦}

Pbgrd

4.56s.

LINK . SPRINGER . COM {}