We are analyzing https://link.springer.com/protocol/10.1007/978-1-4939-3801-8_13.

Title:
Isolation and Characterization of Low- vs. High-Density Neutrophils in Cancer | SpringerLink
Description:
Neutrophils are the most abundant of all white blood cells in the human circulation and serve as the first line of defense against microbial infections. Traditionally, neutrophils were viewed as a homogeneous population of myeloid cells. However, in recent years...
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Keywords {🔍}

pubmed, article, neutrophils, cancer, google, scholar, cas, tumor, research, protocol, granot, central, cells, privacy, cookies, content, information, publish, search, microenvironment, characterization, sagiv, neutrophil, subsets, access, download, springer, usd, personal, data, log, journal, isolation, low, september, voels, zvi, book, biology, lung, cell, patients, author, medical, york, ebook, optional, media, parties, protection,

Topics {✒️}

month download article/chapter medical research israel-canada myeloid-derived suppressor cells sandra voels & zvi granot privacy choices/manage cookies device instant download editor information editors ursini-siegel tumor cells mount european economic area white blood cells antibody based enrichment initial angiogenic switch tumors releasing interleukin-8 sionov rv impaired migratory properties neutrophil chemokines secreted circulating neutrophil subpopulations journal finder publish tumor-bearing mice conditions privacy policy lung antimetastatic response infiltrating neutrophils mediate proinflammatory neutrophils isolated accepting optional cookies low density subpopulations severely infected patients tumor microenvironment jitka medical research main content log high-density neutrophils author correspondence low-density neutrophils myeloid cells low-density granulocytes distinct neutrophil subsets cancer research zvi granot social media permissions reprints check access ethics access humana press protocol usd 49 journal publish privacy policy israel jitka tumor microenvironment chapter log protocol sagiv

Schema {🗺️}

ScholarlyArticle:
      headline:Isolation and Characterization of Low- vs. High-Density Neutrophils in Cancer
      pageEnd:193
      pageStart:179
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-4939-3801-8.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:The Tumor Microenvironment
         isbn:
            978-1-4939-3801-8
            978-1-4939-3799-8
         type:Book
      publisher:
         name:Springer New York
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Jitka Y. Sagiv
            affiliation:
                  name:Hebrew University Medical School
                  address:
                     name:Department of Developmental Biology and Cancer Research, Institute for Medical Research Israel-Canada, Hebrew University Medical School, Jerusalem, Israel
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Sandra Voels
            affiliation:
                  name:Hebrew University Medical School
                  address:
                     name:Department of Developmental Biology and Cancer Research, Institute for Medical Research Israel-Canada, Hebrew University Medical School, Jerusalem, Israel
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Zvi Granot
            affiliation:
                  name:Hebrew University Medical School
                  address:
                     name:Department of Developmental Biology and Cancer Research, Institute for Medical Research Israel-Canada, Hebrew University Medical School, Jerusalem, Israel
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      keywords:Neutrophils, Cancer, Isolation, Density, Enrichment, Flow cytometry, Cytotoxicity
      description:Neutrophils are the most abundant of all white blood cells in the human circulation and serve as the first line of defense against microbial infections. Traditionally, neutrophils were viewed as a homogeneous population of myeloid cells. However, in recent years accumulating evidence has suggested that neutrophils are heterogeneous and that distinct neutrophil subsets may play very different roles. Here, we describe the methodology for isolation of high- and low-density neutrophils from the murine and human circulation using a density gradient and antibody based enrichment. We further describe the methodology for functional characterization of these different neutrophil subsets in the context of cancer.
      datePublished:2016
      isAccessibleForFree:
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         type:WebPageElement
      context:https://schema.org
Book:
      name:The Tumor Microenvironment
      isbn:
         978-1-4939-3801-8
         978-1-4939-3799-8
Organization:
      name:Springer New York
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Hebrew University Medical School
      address:
         name:Department of Developmental Biology and Cancer Research, Institute for Medical Research Israel-Canada, Hebrew University Medical School, Jerusalem, Israel
         type:PostalAddress
      name:Hebrew University Medical School
      address:
         name:Department of Developmental Biology and Cancer Research, Institute for Medical Research Israel-Canada, Hebrew University Medical School, Jerusalem, Israel
         type:PostalAddress
      name:Hebrew University Medical School
      address:
         name:Department of Developmental Biology and Cancer Research, Institute for Medical Research Israel-Canada, Hebrew University Medical School, Jerusalem, Israel
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Jitka Y. Sagiv
      affiliation:
            name:Hebrew University Medical School
            address:
               name:Department of Developmental Biology and Cancer Research, Institute for Medical Research Israel-Canada, Hebrew University Medical School, Jerusalem, Israel
               type:PostalAddress
            type:Organization
      name:Sandra Voels
      affiliation:
            name:Hebrew University Medical School
            address:
               name:Department of Developmental Biology and Cancer Research, Institute for Medical Research Israel-Canada, Hebrew University Medical School, Jerusalem, Israel
               type:PostalAddress
            type:Organization
      name:Zvi Granot
      affiliation:
            name:Hebrew University Medical School
            address:
               name:Department of Developmental Biology and Cancer Research, Institute for Medical Research Israel-Canada, Hebrew University Medical School, Jerusalem, Israel
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Department of Developmental Biology and Cancer Research, Institute for Medical Research Israel-Canada, Hebrew University Medical School, Jerusalem, Israel
      name:Department of Developmental Biology and Cancer Research, Institute for Medical Research Israel-Canada, Hebrew University Medical School, Jerusalem, Israel
      name:Department of Developmental Biology and Cancer Research, Institute for Medical Research Israel-Canada, Hebrew University Medical School, Jerusalem, Israel
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      isAccessibleForFree:
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