We are analyzing https://link.springer.com/protocol/10.1007/978-1-4939-3756-1_15.

Title:
Detection and Analysis of Cell Cycle-Associated APC/C-Mediated Cellular Ubiquitylation In Vitro and In Vivo | SpringerLink
Description:
The anaphase-promoting complex or cyclosome (APC/C) is one of the major orchestrators of the cell division cycle in mammalian cells. The APC/C acts as a ubiquitin ligase that triggers sequential ubiquitylation of a significant number of substrates which will be...
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Topics {✒️}

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Schema {🗺️}

ScholarlyArticle:
      headline:Detection and Analysis of Cell Cycle-Associated APC/C-Mediated Cellular Ubiquitylation In Vitro and In Vivo
      pageEnd:265
      pageStart:251
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-4939-3756-1.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:Proteostasis
         isbn:
            978-1-4939-3756-1
            978-1-4939-3754-7
         type:Book
      publisher:
         name:Springer New York
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Cesyen Cedeño
            affiliation:
                  name:Vrije Universiteit Brussel (VUB)
                  address:
                     name:Department of Structural Biology, Vlaams Instituut voor Biotechnologie (VIB), Vrije Universiteit Brussel (VUB), Brussels, Belgium
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Esther La Monaca
            affiliation:
                  name:Vrije Universiteit Brussel (VUB)
                  address:
                     name:Laboratory of Pathophysiological Cell Signaling (PACS), Department of Biology, Faculty of Sciences and Bioengineering Sciences, Vrije Universiteit Brussel (VUB), Brussels, Belgium
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Mara Esposito
            affiliation:
                  name:Vrije Universiteit Brussel (VUB)
                  address:
                     name:Laboratory of Pathophysiological Cell Signaling (PACS), Department of Biology, Faculty of Sciences and Bioengineering Sciences, Vrije Universiteit Brussel (VUB), Brussels, Belgium
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Gustavo J. Gutierrez
            affiliation:
                  name:Vrije Universiteit Brussel (VUB)
                  address:
                     name:Laboratory of Pathophysiological Cell Signaling (PACS), Department of Biology, Faculty of Sciences and Bioengineering Sciences, Vrije Universiteit Brussel (VUB), Brussels, Belgium
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      keywords:Anaphase-promoting complex or cyclosome (APC/C), Cell cycle, Ubiquitin, Ubiquitylation, Phosphorylation, Cdc27/APC3, Cdh1, Cdc20
      description:The anaphase-promoting complex or cyclosome (APC/C) is one of the major orchestrators of the cell division cycle in mammalian cells. The APC/C acts as a ubiquitin ligase that triggers sequential ubiquitylation of a significant number of substrates which will be eventually degraded by proteasomes during major transitions of the cell cycle. In this chapter, we present accessible methodologies to assess both in in vitro conditions and in cellular systems ubiquitylation reactions mediated by the APC/C. In addition, we also describe techniques to evidence the changes in protein stability provoked by modulation of the activity of the APC/C. Finally, specific methods to analyze interactors or posttranslational modifications of particular APC/C subunits are also discussed. Given the crucial role played by the APC/C in the regulation of the cell cycle, this review only focuses on its action and effects in actively proliferating cells.
      datePublished:2016
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Book:
      name:Proteostasis
      isbn:
         978-1-4939-3756-1
         978-1-4939-3754-7
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      name:Springer New York
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         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
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      name:Vrije Universiteit Brussel (VUB)
      address:
         name:Department of Structural Biology, Vlaams Instituut voor Biotechnologie (VIB), Vrije Universiteit Brussel (VUB), Brussels, Belgium
         type:PostalAddress
      name:Vrije Universiteit Brussel (VUB)
      address:
         name:Laboratory of Pathophysiological Cell Signaling (PACS), Department of Biology, Faculty of Sciences and Bioengineering Sciences, Vrije Universiteit Brussel (VUB), Brussels, Belgium
         type:PostalAddress
      name:Vrije Universiteit Brussel (VUB)
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         name:Laboratory of Pathophysiological Cell Signaling (PACS), Department of Biology, Faculty of Sciences and Bioengineering Sciences, Vrije Universiteit Brussel (VUB), Brussels, Belgium
         type:PostalAddress
      name:Vrije Universiteit Brussel (VUB)
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         name:Laboratory of Pathophysiological Cell Signaling (PACS), Department of Biology, Faculty of Sciences and Bioengineering Sciences, Vrije Universiteit Brussel (VUB), Brussels, Belgium
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      name:Cesyen Cedeño
      affiliation:
            name:Vrije Universiteit Brussel (VUB)
            address:
               name:Department of Structural Biology, Vlaams Instituut voor Biotechnologie (VIB), Vrije Universiteit Brussel (VUB), Brussels, Belgium
               type:PostalAddress
            type:Organization
      name:Esther La Monaca
      affiliation:
            name:Vrije Universiteit Brussel (VUB)
            address:
               name:Laboratory of Pathophysiological Cell Signaling (PACS), Department of Biology, Faculty of Sciences and Bioengineering Sciences, Vrije Universiteit Brussel (VUB), Brussels, Belgium
               type:PostalAddress
            type:Organization
      name:Mara Esposito
      affiliation:
            name:Vrije Universiteit Brussel (VUB)
            address:
               name:Laboratory of Pathophysiological Cell Signaling (PACS), Department of Biology, Faculty of Sciences and Bioengineering Sciences, Vrije Universiteit Brussel (VUB), Brussels, Belgium
               type:PostalAddress
            type:Organization
      name:Gustavo J. Gutierrez
      affiliation:
            name:Vrije Universiteit Brussel (VUB)
            address:
               name:Laboratory of Pathophysiological Cell Signaling (PACS), Department of Biology, Faculty of Sciences and Bioengineering Sciences, Vrije Universiteit Brussel (VUB), Brussels, Belgium
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Department of Structural Biology, Vlaams Instituut voor Biotechnologie (VIB), Vrije Universiteit Brussel (VUB), Brussels, Belgium
      name:Laboratory of Pathophysiological Cell Signaling (PACS), Department of Biology, Faculty of Sciences and Bioengineering Sciences, Vrije Universiteit Brussel (VUB), Brussels, Belgium
      name:Laboratory of Pathophysiological Cell Signaling (PACS), Department of Biology, Faculty of Sciences and Bioengineering Sciences, Vrije Universiteit Brussel (VUB), Brussels, Belgium
      name:Laboratory of Pathophysiological Cell Signaling (PACS), Department of Biology, Faculty of Sciences and Bioengineering Sciences, Vrije Universiteit Brussel (VUB), Brussels, Belgium
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