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Title:
Production and Concentration of Lentivirus for Transduction of Primary Human T Cells | SpringerLink
Description:
Lentiviral vectors have emerged as efficient tools for investigating T cell biology through their ability to efficiently deliver transgene expression into both dividing and nondividing cells. Such lentiviral vectors have the potential to infect a wide variety of cell...
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Keywords {🔍}

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Topics {✒️}

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Schema {🗺️}

ScholarlyArticle:
      headline:Production and Concentration of Lentivirus for Transduction of Primary Human T Cells
      pageEnd:93
      pageStart:85
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-4939-3753-0.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools
         isbn:
            978-1-4939-3753-0
            978-1-4939-3751-6
         type:Book
      publisher:
         name:Springer New York
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Alan Kennedy
            affiliation:
                  name:University College London Division of Infection and Immunity
                  address:
                     name:Institute of Immunity and Transplantation, University College London Division of Infection and Immunity, London, UK
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Adam P. Cribbs
            affiliation:
                  name:University of Oxford
                  address:
                     name:Kennedy Institute of Rheumatology, University of Oxford, Oxford, UK
                     type:PostalAddress
                  type:Organization
                  name:Nuffield Orthopaedic Centre
                  address:
                     name:Botnar Research Centre, Nuffield Orthopaedic Centre, Oxford, UK
                     type:PostalAddress
                  type:Organization
                  name:MRC Functional Genomics Unit, Computational Genomics and Training Centre (CGAT)
                  address:
                     name:Department of Physiology, Anatomy and Genetics, MRC Functional Genomics Unit, Computational Genomics and Training Centre (CGAT), Oxford, UK
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      keywords:Lentivirus, Primary, CD4+ T cells, CD45RA+ , Titer, Human T cell, Lentiviral vector
      description:Lentiviral vectors have emerged as efficient tools for investigating T cell biology through their ability to efficiently deliver transgene expression into both dividing and nondividing cells. Such lentiviral vectors have the potential to infect a wide variety of cell types. However, despite this advantage, the ability to transduce primary human T cells remains challenging and methods to achieve efficient gene transfer are often time consuming and expensive. We describe a method for generating lentivirus that is simple to perform and does not require the purchase of non-standard equipment to transduce primary human T cells. Therefore, we provide an optimized protocol that is easy to implement and allow transduction with high efficiency and reproducibility.
      datePublished:2016
      isAccessibleForFree:
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         type:WebPageElement
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Book:
      name:Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools
      isbn:
         978-1-4939-3753-0
         978-1-4939-3751-6
Organization:
      name:Springer New York
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:University College London Division of Infection and Immunity
      address:
         name:Institute of Immunity and Transplantation, University College London Division of Infection and Immunity, London, UK
         type:PostalAddress
      name:University of Oxford
      address:
         name:Kennedy Institute of Rheumatology, University of Oxford, Oxford, UK
         type:PostalAddress
      name:Nuffield Orthopaedic Centre
      address:
         name:Botnar Research Centre, Nuffield Orthopaedic Centre, Oxford, UK
         type:PostalAddress
      name:MRC Functional Genomics Unit, Computational Genomics and Training Centre (CGAT)
      address:
         name:Department of Physiology, Anatomy and Genetics, MRC Functional Genomics Unit, Computational Genomics and Training Centre (CGAT), Oxford, UK
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Alan Kennedy
      affiliation:
            name:University College London Division of Infection and Immunity
            address:
               name:Institute of Immunity and Transplantation, University College London Division of Infection and Immunity, London, UK
               type:PostalAddress
            type:Organization
      name:Adam P. Cribbs
      affiliation:
            name:University of Oxford
            address:
               name:Kennedy Institute of Rheumatology, University of Oxford, Oxford, UK
               type:PostalAddress
            type:Organization
            name:Nuffield Orthopaedic Centre
            address:
               name:Botnar Research Centre, Nuffield Orthopaedic Centre, Oxford, UK
               type:PostalAddress
            type:Organization
            name:MRC Functional Genomics Unit, Computational Genomics and Training Centre (CGAT)
            address:
               name:Department of Physiology, Anatomy and Genetics, MRC Functional Genomics Unit, Computational Genomics and Training Centre (CGAT), Oxford, UK
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Institute of Immunity and Transplantation, University College London Division of Infection and Immunity, London, UK
      name:Kennedy Institute of Rheumatology, University of Oxford, Oxford, UK
      name:Botnar Research Centre, Nuffield Orthopaedic Centre, Oxford, UK
      name:Department of Physiology, Anatomy and Genetics, MRC Functional Genomics Unit, Computational Genomics and Training Centre (CGAT), Oxford, UK
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

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