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Cross-Presentation Assay for Human Dendritic Cells | SpringerLink
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The presentation of exogenous antigens on major histocompatibility complex (MHC) class I molecules, termed cross-presentation, is essential for the initiation of cytotoxic immune responses. Numerous studies in mice and human have shown that dendritic cells are the...
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ScholarlyArticle:
headline:Cross-Presentation Assay for Human Dendritic Cells
pageEnd:198
pageStart:189
image:https://media.springernature.com/w153/springer-static/cover/book/978-1-4939-3606-9.jpg
genre:
Springer Protocols
isPartOf:
name:Dendritic Cell Protocols
isbn:
978-1-4939-3606-9
978-1-4939-3604-5
type:Book
publisher:
name:Springer New York
logo:
url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
type:ImageObject
type:Organization
author:
name:Elodie Segura
affiliation:
name:INSERM U932, and Institut Curie, Centre de recherche
address:
name:INSERM U932, and Institut Curie, Centre de recherche, Paris Cedex 05, France
type:PostalAddress
type:Organization
email:[email protected]
type:Person
keywords:Dendritic cells, Cross-presentation, Antigen processing, Human, CD8 T cells
description:The presentation of exogenous antigens on major histocompatibility complex (MHC) class I molecules, termed cross-presentation, is essential for the initiation of cytotoxic immune responses. Numerous studies in mice and human have shown that dendritic cells are the best cross-presenting cells. The protocol described here allows the assessment of the cross-presentation by human dendritic cells of a model antigen (either soluble or cell associated) to antigen-specific CD8 T cells.
datePublished:2016
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name:Elodie Segura
affiliation:
name:INSERM U932, and Institut Curie, Centre de recherche
address:
name:INSERM U932, and Institut Curie, Centre de recherche, Paris Cedex 05, France
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