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Title:
Assessing Extracellular ATP as Danger Signal In Vivo: The pmeLuc System | SpringerLink
Description:
Inflammation is the key pathophysiological response triggered by noxious agents in multicellular organisms. Central to inflammation is detection of exogenous or endogenous danger signals by immune cells. Extracellular ATP is a ubiquitous danger signal released during...
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Keywords {🔍}

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Topics {✒️}

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Schema {🗺️}

ScholarlyArticle:
      headline:Assessing Extracellular ATP as Danger Signal In Vivo: The pmeLuc System
      pageEnd:129
      pageStart:115
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-4939-3566-6.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:NLR Proteins
         isbn:
            978-1-4939-3566-6
            978-1-4939-3564-2
         type:Book
      publisher:
         name:Springer New York
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Francesco Di Virgilio
            affiliation:
                  name:University of Ferrara
                  address:
                     name:Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
            name:Paolo Pinton
            affiliation:
                  name:University of Ferrara
                  address:
                     name:Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Simonetta Falzoni
            affiliation:
                  name:University of Ferrara
                  address:
                     name:Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy
                     type:PostalAddress
                  type:Organization
            type:Person
      keywords:Extracellular ATP, Plasma membrane luciferase, Luciferin, Luminescence, Luminometry
      description:Inflammation is the key pathophysiological response triggered by noxious agents in multicellular organisms. Central to inflammation is detection of exogenous or endogenous danger signals by immune cells. Extracellular ATP is a ubiquitous danger signal released during septic or sterile inflammation. The development of reliable techniques to measure extracellular ATP in vivo has become an urgent need in inflammation studies after the discovery that the most potent plasma membrane receptor responsible for NLRP3 inflammasome activation is an ATP-activated receptor, P2RX7. Here we describe an easy bioluminescence technique for the measurement of extracellular ATP in vivo.
      datePublished:2016
      isAccessibleForFree:
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Book:
      name:NLR Proteins
      isbn:
         978-1-4939-3566-6
         978-1-4939-3564-2
Organization:
      name:Springer New York
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:University of Ferrara
      address:
         name:Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy
         type:PostalAddress
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      address:
         name:Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy
         type:PostalAddress
      name:University of Ferrara
      address:
         name:Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy
         type:PostalAddress
ImageObject:
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Person:
      name:Francesco Di Virgilio
      affiliation:
            name:University of Ferrara
            address:
               name:Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy
               type:PostalAddress
            type:Organization
      email:[email protected]
      name:Paolo Pinton
      affiliation:
            name:University of Ferrara
            address:
               name:Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy
               type:PostalAddress
            type:Organization
      name:Simonetta Falzoni
      affiliation:
            name:University of Ferrara
            address:
               name:Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy
               type:PostalAddress
            type:Organization
PostalAddress:
      name:Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy
      name:Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy
      name:Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy
WebPageElement:
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