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Title:
Single-Molecule Imaging of Signal Transduction via GPI-Anchored Receptors | SpringerLink
Description:
Lipid rafts have been drawing extensive attention as a signaling platform. To investigate molecular interactions in lipid rafts, we often need to observe molecules in the plasma membranes of living cells because chemical fixation and subsequent immunostaining with...
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Keywords {🔍}

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Topics {✒️}

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Schema {🗺️}

ScholarlyArticle:
      headline:Single-Molecule Imaging of Signal Transduction via GPI-Anchored Receptors
      pageEnd:238
      pageStart:229
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-4939-3170-5.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:Lipid Signaling Protocols
         isbn:
            978-1-4939-3170-5
            978-1-4939-3169-9
         type:Book
      publisher:
         name:Springer New York
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Kenichi G. N. Suzuki
            affiliation:
                  name:Kyoto University
                  address:
                     name:Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan
                     type:PostalAddress
                  type:Organization
                  name:National Centre for Biological Sciences (NCBS)/Institute for Stem Cell Biology and Regenerative Medicine (inStem)
                  address:
                     name:National Centre for Biological Sciences (NCBS)/Institute for Stem Cell Biology and Regenerative Medicine (inStem), Bangalore, India
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      keywords: Single-particle track ing , Single-molecule imaging, GPI-anchored protein, Rafts, Colocalization , Temporal confinement , Src family kinase , PLCγ, IP , Calcium
      description:Lipid rafts have been drawing extensive attention as a signaling platform. To investigate molecular interactions in lipid rafts, we often need to observe molecules in the plasma membranes of living cells because chemical fixation and subsequent immunostaining with divalent or multivalent antibodies may change the location of the target molecules. In this chapter, we describe how to examine dynamics of raft-associated glycosylphosphatidylinositol (GPI)-anchored receptors and interactions of the receptors with downstream signaling molecules by single-particle tracking or single-molecule imaging techniques.
      datePublished:2016
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Book:
      name:Lipid Signaling Protocols
      isbn:
         978-1-4939-3170-5
         978-1-4939-3169-9
Organization:
      name:Springer New York
      logo:
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         type:ImageObject
      name:Kyoto University
      address:
         name:Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan
         type:PostalAddress
      name:National Centre for Biological Sciences (NCBS)/Institute for Stem Cell Biology and Regenerative Medicine (inStem)
      address:
         name:National Centre for Biological Sciences (NCBS)/Institute for Stem Cell Biology and Regenerative Medicine (inStem), Bangalore, India
         type:PostalAddress
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Person:
      name:Kenichi G. N. Suzuki
      affiliation:
            name:Kyoto University
            address:
               name:Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan
               type:PostalAddress
            type:Organization
            name:National Centre for Biological Sciences (NCBS)/Institute for Stem Cell Biology and Regenerative Medicine (inStem)
            address:
               name:National Centre for Biological Sciences (NCBS)/Institute for Stem Cell Biology and Regenerative Medicine (inStem), Bangalore, India
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan
      name:National Centre for Biological Sciences (NCBS)/Institute for Stem Cell Biology and Regenerative Medicine (inStem), Bangalore, India
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