We are analyzing https://link.springer.com/protocol/10.1007/978-1-4939-3040-1_11.

Title:
Engineered mtZFNs for Manipulation of Human Mitochondrial DNA Heteroplasmy | SpringerLink
Description:
Enrichment of desired mitochondrial DNA (mtDNA) haplotypes, in both experimental systems and the clinic, is an end sought by many. Through use of a designer nuclease platform optimized for delivery to mitochondria—the mitochondrially targeted zinc...
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Keywords {🔍}

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Topics {✒️}

selection-free zinc-finger-nuclease engineering quasi-dimeric zinc-finger nuclease enhancing zinc-finger-nuclease activity month download article/chapter rapid “open-source” engineering engineered zinc-finger proteins wellcome trust/mrc building preassembled zinc-finger arrays repetitive zinc-binding domains real-time quantitative pcr customized zinc-finger nucleases mitochondrial dna heteroplasmy mitochondrially targeted zfns mutant mitochondrial genomes zinc-finger nucleases zinc finger nucleases human mitochondrial genome device instant download zinc finger fusions desired mitochondrial dna privacy choices/manage cookies editor information editors chimeric restriction endonuclease assess mtzfn activity engineered mtzfns pruett-miller sm hybrid restriction enzymes sequence-specific modification michal minczuk european economic area single nucleotide substitution structure-based redesign dimerization interface reduces sander jd maeder ml authors correspondence protocol cite journal finder publish conditions privacy policy van haute mammalian cell cultures mccray pb jr de vivo dc mitochondrial dna accepting optional cookies miller jc context-dependent assembly rapid directional shift medical research protocol usd 49

Schema {🗺️}

ScholarlyArticle:
      headline:Engineered mtZFNs for Manipulation of Human Mitochondrial DNA Heteroplasmy
      pageEnd:162
      pageStart:145
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-4939-3040-1.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:Mitochondrial DNA
         isbn:
            978-1-4939-3040-1
            978-1-4939-3039-5
         type:Book
      publisher:
         name:Springer New York
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Payam A. Gammage
            affiliation:
                  name:MRC Mitochondrial Biology Unit
                  address:
                     name:MRC Mitochondrial Biology Unit, Cambridge, UK
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
            name:Lindsey Van Haute
            affiliation:
                  name:MRC Mitochondrial Biology Unit
                  address:
                     name:MRC Mitochondrial Biology Unit, Cambridge, UK
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Michal Minczuk
            affiliation:
                  name:MRC Mitochondrial Biology Unit
                  address:
                     name:MRC Mitochondrial Biology Unit, Cambridge, UK
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      keywords: Genetic engineering , Zinc finger, Zinc finger nuclease , Mitochondrial disease , Heteroplasmy, Gene therapy
      description:Enrichment of desired mitochondrial DNA (mtDNA) haplotypes, in both experimental systems and the clinic, is an end sought by many. Through use of a designer nuclease platform optimized for delivery to mitochondria—the mitochondrially targeted zinc finger-nuclease (mtZFN)—it is possible to discriminate between mtDNA haplotypes with specificity to the order of a single nucleotide substitution. Site-specific cleavage of DNA produces a shift in the heteroplasmic ratio in favor of the untargeted haplotype. Here, we describe protocols for assembly of paired, conventional tail–tail mtZFN constructs and experimental approaches to assess mtZFN activity in mammalian cell cultures.
      datePublished:2016
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Book:
      name:Mitochondrial DNA
      isbn:
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         978-1-4939-3039-5
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      name:Springer New York
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      name:Payam A. Gammage
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            address:
               name:MRC Mitochondrial Biology Unit, Cambridge, UK
               type:PostalAddress
            type:Organization
      email:[email protected]
      name:Lindsey Van Haute
      affiliation:
            name:MRC Mitochondrial Biology Unit
            address:
               name:MRC Mitochondrial Biology Unit, Cambridge, UK
               type:PostalAddress
            type:Organization
      name:Michal Minczuk
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            name:MRC Mitochondrial Biology Unit
            address:
               name:MRC Mitochondrial Biology Unit, Cambridge, UK
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:MRC Mitochondrial Biology Unit, Cambridge, UK
      name:MRC Mitochondrial Biology Unit, Cambridge, UK
      name:MRC Mitochondrial Biology Unit, Cambridge, UK
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