We are analyzing https://link.springer.com/protocol/10.1007/978-1-4939-1133-2_10.

Title:
Metabolic Characterization of Hematopoietic Stem Cells | SpringerLink
Description:
An important feature of stem cells is their maintenance in their respective hypoxic niche. Survival in this low-oxygen microenvironment requires significant metabolic adaptation. We demonstrated that mouse HSCs utilize glycolysis instead of mitochondrial oxidative...
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28 years and 1 months (reg. 1997-05-29).

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Keywords {🔍}

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Topics {✒️}

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Schema {🗺️}

ScholarlyArticle:
      headline:Metabolic Characterization of Hematopoietic Stem Cells
      pageEnd:164
      pageStart:155
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-4939-1133-2.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:Hematopoietic Stem Cell Protocols
         isbn:
            978-1-4939-1133-2
            978-1-4939-1132-5
         type:Book
      publisher:
         name:Springer New York
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Fatih Kocabas
            affiliation:
                  name:North American University
                  address:
                     name:Department of Education, and Texas Institute of Biotechnology, Education and Research, North American University, Houston, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Junke Zheng
            affiliation:
                  name:Shanghai Jiao-Tong University School of Medicine
                  address:
                     name:Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Shanghai Jiao-Tong University School of Medicine, Shanghai, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Chengcheng Zhang
            affiliation:
                  name:University of Texas Southwestern Medical Center
                  address:
                     name:Departments of Physiology and Developmental Biology, University of Texas Southwestern Medical Center, Dallas, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Hesham A. Sadek
            affiliation:
                  name:University of Texas Southwestern Medical Center
                  address:
                     name:Department of Internal Medicine, Division of Cardiology, University of Texas Southwestern Medical Center, Dallas, USA
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      keywords:Hematopoietic stem cells, HSC, Stem cell metabolism, Reactive oxygen species, ROS, Glycolysis, ATP content, Oxygen consumption, NADH, Glycolytic flux, Lactate
      description:An important feature of stem cells is their maintenance in their respective hypoxic niche. Survival in this low-oxygen microenvironment requires significant metabolic adaptation. We demonstrated that mouse HSCs utilize glycolysis instead of mitochondrial oxidative phosphorylation to meet their energy demands. We have adapted various tools for characterization of the metabolic properties of hematopoietic stem cells (HSCs). These techniques include flow cytometric profiling of HSCs based on mitochondrial potential and NADH fluorescence as well as measurement of ATP content, oxygen consumption rate, and glycolytic flux in purified HSCs.
      datePublished:2014
      isAccessibleForFree:
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         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
      context:https://schema.org
Book:
      name:Hematopoietic Stem Cell Protocols
      isbn:
         978-1-4939-1133-2
         978-1-4939-1132-5
Organization:
      name:Springer New York
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:North American University
      address:
         name:Department of Education, and Texas Institute of Biotechnology, Education and Research, North American University, Houston, USA
         type:PostalAddress
      name:Shanghai Jiao-Tong University School of Medicine
      address:
         name:Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Shanghai Jiao-Tong University School of Medicine, Shanghai, China
         type:PostalAddress
      name:University of Texas Southwestern Medical Center
      address:
         name:Departments of Physiology and Developmental Biology, University of Texas Southwestern Medical Center, Dallas, USA
         type:PostalAddress
      name:University of Texas Southwestern Medical Center
      address:
         name:Department of Internal Medicine, Division of Cardiology, University of Texas Southwestern Medical Center, Dallas, USA
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Fatih Kocabas
      affiliation:
            name:North American University
            address:
               name:Department of Education, and Texas Institute of Biotechnology, Education and Research, North American University, Houston, USA
               type:PostalAddress
            type:Organization
      name:Junke Zheng
      affiliation:
            name:Shanghai Jiao-Tong University School of Medicine
            address:
               name:Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Shanghai Jiao-Tong University School of Medicine, Shanghai, China
               type:PostalAddress
            type:Organization
      name:Chengcheng Zhang
      affiliation:
            name:University of Texas Southwestern Medical Center
            address:
               name:Departments of Physiology and Developmental Biology, University of Texas Southwestern Medical Center, Dallas, USA
               type:PostalAddress
            type:Organization
      name:Hesham A. Sadek
      affiliation:
            name:University of Texas Southwestern Medical Center
            address:
               name:Department of Internal Medicine, Division of Cardiology, University of Texas Southwestern Medical Center, Dallas, USA
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Department of Education, and Texas Institute of Biotechnology, Education and Research, North American University, Houston, USA
      name:Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Shanghai Jiao-Tong University School of Medicine, Shanghai, China
      name:Departments of Physiology and Developmental Biology, University of Texas Southwestern Medical Center, Dallas, USA
      name:Department of Internal Medicine, Division of Cardiology, University of Texas Southwestern Medical Center, Dallas, USA
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

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