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Title:
Intracellular Potassium Ion Measurements by Inductively Coupled Plasma Optical Emission Spectrometer (ICP-OES) | SpringerLink
Description:
Potassium ion (K+) efflux is often considered as an upstream signaling event of NLRP3 activation. The main evidence to demonstrate the importance of K+ efflux is that high concentration of extracellular K+ inhibits NLRP3 inflammasome assembly. However, the conditions...
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Schema {🗺️}

ScholarlyArticle:
      headline:Intracellular Potassium Ion Measurements by Inductively Coupled Plasma Optical Emission Spectrometer (ICP-OES)
      pageEnd:92
      pageStart:85
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-0716-2144-8.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:The Inflammasome
         isbn:
            978-1-0716-2144-8
            978-1-0716-2143-1
         type:Book
      publisher:
         name:Springer US
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Yifei Zhang
            affiliation:
                  name:Tianjin University
                  address:
                     name:Academy of Medical Engineering and Translational Medicine, Tianjin University, Tianjin, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Yan Shi
            affiliation:
                  name:Tsinghua University
                  address:
                     name:Tsinghua Institute for Immunology and Department of Basic Medical Sciences, Beijing Key Lab for Immunological Research on Chronic Diseases, School of Medicine, Tsinghua University, Beijing, China
                     type:PostalAddress
                  type:Organization
                  name:Tsinghua University
                  address:
                     name:Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, China
                     type:PostalAddress
                  type:Organization
                  name:University of Calgary
                  address:
                     name:Department of Microbiology, Immunology and Infectious Diseases, University of Calgary, Calgary, Canada
                     type:PostalAddress
                  type:Organization
                  name:University of Calgary
                  address:
                     name:Snyder Institute, University of Calgary, Calgary, Canada
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      keywords:NLRP3 inflammasome, Intracellular K+ content
      description:Potassium ion (K+) efflux is often considered as an upstream signaling event of NLRP3 activation. The main evidence to demonstrate the importance of K+ efflux is that high concentration of extracellular K+ inhibits NLRP3 inflammasome assembly. However, the conditions used to prevent K+ flowing also breaks down a basic parameter of eukaryotic biology, leading to sustained membrane potential depolarization and affecting normal signal transduction in cells. Therefore, direct measurement of intracellular ion concentration can more truly reflect the role of K+ flow during the activation of NLRP3. In this chapter, we will provide the rationale and a method to evaluate intracellular K+ concentration by ICP-OES (Inductively Coupled Plasma Optical Emission Spectroscopy), which helps us understand how disturbances in intracellular K+ level orchestrates NLRP3 inflammasome activation.
      datePublished:2022
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Book:
      name:The Inflammasome
      isbn:
         978-1-0716-2144-8
         978-1-0716-2143-1
Organization:
      name:Springer US
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Tianjin University
      address:
         name:Academy of Medical Engineering and Translational Medicine, Tianjin University, Tianjin, China
         type:PostalAddress
      name:Tsinghua University
      address:
         name:Tsinghua Institute for Immunology and Department of Basic Medical Sciences, Beijing Key Lab for Immunological Research on Chronic Diseases, School of Medicine, Tsinghua University, Beijing, China
         type:PostalAddress
      name:Tsinghua University
      address:
         name:Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, China
         type:PostalAddress
      name:University of Calgary
      address:
         name:Department of Microbiology, Immunology and Infectious Diseases, University of Calgary, Calgary, Canada
         type:PostalAddress
      name:University of Calgary
      address:
         name:Snyder Institute, University of Calgary, Calgary, Canada
         type:PostalAddress
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      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Yifei Zhang
      affiliation:
            name:Tianjin University
            address:
               name:Academy of Medical Engineering and Translational Medicine, Tianjin University, Tianjin, China
               type:PostalAddress
            type:Organization
      name:Yan Shi
      affiliation:
            name:Tsinghua University
            address:
               name:Tsinghua Institute for Immunology and Department of Basic Medical Sciences, Beijing Key Lab for Immunological Research on Chronic Diseases, School of Medicine, Tsinghua University, Beijing, China
               type:PostalAddress
            type:Organization
            name:Tsinghua University
            address:
               name:Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, China
               type:PostalAddress
            type:Organization
            name:University of Calgary
            address:
               name:Department of Microbiology, Immunology and Infectious Diseases, University of Calgary, Calgary, Canada
               type:PostalAddress
            type:Organization
            name:University of Calgary
            address:
               name:Snyder Institute, University of Calgary, Calgary, Canada
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Academy of Medical Engineering and Translational Medicine, Tianjin University, Tianjin, China
      name:Tsinghua Institute for Immunology and Department of Basic Medical Sciences, Beijing Key Lab for Immunological Research on Chronic Diseases, School of Medicine, Tsinghua University, Beijing, China
      name:Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, China
      name:Department of Microbiology, Immunology and Infectious Diseases, University of Calgary, Calgary, Canada
      name:Snyder Institute, University of Calgary, Calgary, Canada
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