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Title:
Experimental and Computational Considerations in the Study of RNA-Binding Protein-RNA Interactions | SpringerLink
Description:
After an RNA is transcribed, it undergoes a variety of processing steps that can change the encoded protein sequence (through alternative splicing and RNA editing), regulate the stability of the RNA, and control subcellular localization, timing, and rate of...
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Topics {✒️}

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Schema {🗺️}

ScholarlyArticle:
      headline:Experimental and Computational Considerations in the Study of RNA-Binding Protein-RNA Interactions
      pageEnd:28
      pageStart:1
      image:https://media.springernature.com/w153/springer-static/cover/book/978-3-319-29073-7.jpg
      genre:
         Biomedical and Life Sciences
         Biomedical and Life Sciences (R0)
      isPartOf:
         name:RNA Processing
         isbn:
            978-3-319-29073-7
            978-3-319-29071-3
         type:Book
      publisher:
         name:Springer International Publishing
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Eric L. Van Nostrand
            affiliation:
                  name:Stem Cell Program and Institute for Genomic Medicine, University of California
                  address:
                     name:Department of Cellular and Molecular Medicine, Stem Cell Program and Institute for Genomic Medicine, University of California, La Jolla, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Stephanie C. Huelga
            affiliation:
                  name:Stem Cell Program and Institute for Genomic Medicine, University of California
                  address:
                     name:Department of Cellular and Molecular Medicine, Stem Cell Program and Institute for Genomic Medicine, University of California, La Jolla, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Gene W. Yeo
            affiliation:
                  name:Stem Cell Program and Institute for Genomic Medicine, University of California
                  address:
                     name:Department of Cellular and Molecular Medicine, Stem Cell Program and Institute for Genomic Medicine, University of California, La Jolla, USA
                     type:PostalAddress
                  type:Organization
                  name:Molecular Engineering Laboratory, A*STAR
                  address:
                     name:Molecular Engineering Laboratory, A*STAR, Singapore, Singapore
                     type:PostalAddress
                  type:Organization
                  name:Yong Loo Lin School of Medicine, National University of Singapore
                  address:
                     name:Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      keywords:CLIP-seq, eCLIP, HITS-CLIP, PAR-CLIP, iCLIP, Ribo-seq, BRIC_seq, RNA-seq, Microarray, RNA-binding protein, RNA processing
      description:After an RNA is transcribed, it undergoes a variety of processing steps that can change the encoded protein sequence (through alternative splicing and RNA editing), regulate the stability of the RNA, and control subcellular localization, timing, and rate of translation. The recent explosion in genomics techniques has enabled transcriptome-wide profiling of RNA processing in an unbiased manner. However, it has also brought with it both experimental challenges in developing improved methods to probe distinct processing steps, as well as computational challenges in data storage, processing, and analysis tools to enable large-scale interpretation in the genomics era. In this chapter we review experimental techniques and challenges in profiling various aspects of RNA processing, as well as recent efforts to develop analyses integrating multiple data sources and techniques to infer RNA regulatory networks.
      datePublished:2016
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      name:RNA Processing
      isbn:
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         978-3-319-29071-3
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      name:Springer International Publishing
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         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Stem Cell Program and Institute for Genomic Medicine, University of California
      address:
         name:Department of Cellular and Molecular Medicine, Stem Cell Program and Institute for Genomic Medicine, University of California, La Jolla, USA
         type:PostalAddress
      name:Stem Cell Program and Institute for Genomic Medicine, University of California
      address:
         name:Department of Cellular and Molecular Medicine, Stem Cell Program and Institute for Genomic Medicine, University of California, La Jolla, USA
         type:PostalAddress
      name:Stem Cell Program and Institute for Genomic Medicine, University of California
      address:
         name:Department of Cellular and Molecular Medicine, Stem Cell Program and Institute for Genomic Medicine, University of California, La Jolla, USA
         type:PostalAddress
      name:Molecular Engineering Laboratory, A*STAR
      address:
         name:Molecular Engineering Laboratory, A*STAR, Singapore, Singapore
         type:PostalAddress
      name:Yong Loo Lin School of Medicine, National University of Singapore
      address:
         name:Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore
         type:PostalAddress
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Person:
      name:Eric L. Van Nostrand
      affiliation:
            name:Stem Cell Program and Institute for Genomic Medicine, University of California
            address:
               name:Department of Cellular and Molecular Medicine, Stem Cell Program and Institute for Genomic Medicine, University of California, La Jolla, USA
               type:PostalAddress
            type:Organization
      name:Stephanie C. Huelga
      affiliation:
            name:Stem Cell Program and Institute for Genomic Medicine, University of California
            address:
               name:Department of Cellular and Molecular Medicine, Stem Cell Program and Institute for Genomic Medicine, University of California, La Jolla, USA
               type:PostalAddress
            type:Organization
      name:Gene W. Yeo
      affiliation:
            name:Stem Cell Program and Institute for Genomic Medicine, University of California
            address:
               name:Department of Cellular and Molecular Medicine, Stem Cell Program and Institute for Genomic Medicine, University of California, La Jolla, USA
               type:PostalAddress
            type:Organization
            name:Molecular Engineering Laboratory, A*STAR
            address:
               name:Molecular Engineering Laboratory, A*STAR, Singapore, Singapore
               type:PostalAddress
            type:Organization
            name:Yong Loo Lin School of Medicine, National University of Singapore
            address:
               name:Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Department of Cellular and Molecular Medicine, Stem Cell Program and Institute for Genomic Medicine, University of California, La Jolla, USA
      name:Department of Cellular and Molecular Medicine, Stem Cell Program and Institute for Genomic Medicine, University of California, La Jolla, USA
      name:Department of Cellular and Molecular Medicine, Stem Cell Program and Institute for Genomic Medicine, University of California, La Jolla, USA
      name:Molecular Engineering Laboratory, A*STAR, Singapore, Singapore
      name:Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore
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