We are analyzing https://link.springer.com/article/10.1186/s13619-021-00094-4.

Title:
Establishment of porcine and monkey colonic organoids for drug toxicity study | Cell Regeneration
Description:
Pig and monkey are widely used models for exploration of human diseases and evaluation of drug efficiency and toxicity, but high cost limits their uses. Organoids have been shown to be promising models for drug test as they reasonably preserve tissue structure and functions. However, colonic organoids of pig and monkey are not yet established. Here, we report a culture medium to support the growth of porcine and monkey colonic organoids. Wnt signaling and PGE2 are important for long-term expansion of the organoids, and their withdrawal results in lineage differentiation to mature cells. Furthermore, we observe that porcine colonic organoids are closer to human colonic organoids in terms of drug toxicity response. Successful establishment of porcine and monkey colonic organoids would facilitate the mechanistic investigation of the homeostatic regulation of the intestine of these animals and is useful for drug development and toxicity studies.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Education
Science
Non-Profit & Charity

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
However, some sources were not loaded, we suggest to reload the page to get complete results.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We don’t know how the website earns money.

While profit motivates many websites, others exist to inspire, entertain, or provide valuable resources. Websites have a variety of goals. And this might be one of them. Link.springer.com might be earning cash quietly, but we haven't detected the monetization method.

Keywords {🔍}

organoids, pubmed, cell, article, google, scholar, colonic, cas, differentiation, human, porcine, monkey, medium, cells, drug, stem, intestinal, tissue, pcos, toxicity, fig, colon, research, expansion, wang, models, expression, data, culture, organoid, central, animal, size, compared, van, study, growth, model, days, pig, pge, withdrawal, cancer, staining, china, chen, mature, sato, increased, goblet,

Topics {✒️}

niche-independent high-purity cultures ye-guang chen anti-egfr monoclonal antibody inducible cre-mediated recombination van der flier fluorescein-labeled secondary antibodies article download pdf real-time pcr reactions mouse anti-e-cadherin niche-inspired culture condition intestinal stem cells representative bright-field images mediates drug metabolism high cost limits stem cells markers state key laboratory anti-colonic cancer drugs intestinal stem cell 500 ng/ml r-spondin-1 l-wnt3a cell line stem cell culture stem cell markers cell stem cell animal ethics committee cell-titer reagent national key research diseases search search privacy choices/manage cookies high proliferation state adenomatous polyposis coli cell fate determination published article dna topoisomerase inhibitor intestinal epithelial regeneration multiple intestinal neoplasia differentiated mature cells full access ta cell markers digestive disease research veterinary pharmaceutical products drug toxicity response yunnan key laboratory primate biomedical research annu rev pathol health guangdong laboratory related subjects epidermal growth factor biol open chicken intestinal organoids creative commons licence

Schema {🗺️}

WebPage:
      mainEntity:
         headline:Establishment of porcine and monkey colonic organoids for drug toxicity study
         description:Pig and monkey are widely used models for exploration of human diseases and evaluation of drug efficiency and toxicity, but high cost limits their uses. Organoids have been shown to be promising models for drug test as they reasonably preserve tissue structure and functions. However, colonic organoids of pig and monkey are not yet established. Here, we report a culture medium to support the growth of porcine and monkey colonic organoids. Wnt signaling and PGE2 are important for long-term expansion of the organoids, and their withdrawal results in lineage differentiation to mature cells. Furthermore, we observe that porcine colonic organoids are closer to human colonic organoids in terms of drug toxicity response. Successful establishment of porcine and monkey colonic organoids would facilitate the mechanistic investigation of the homeostatic regulation of the intestine of these animals and is useful for drug development and toxicity studies.
         datePublished:2021-10-02T00:00:00Z
         dateModified:2021-10-02T00:00:00Z
         pageStart:1
         pageEnd:10
         license:http://creativecommons.org/publicdomain/zero/1.0/
         sameAs:https://doi.org/10.1186/s13619-021-00094-4
         keywords:
            Pig
            Monkey
            Colonic organoids
            Culture
            Drug toxicity
            Cell Biology
            Stem Cells
            Biomedicine
            general
         image:
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs13619-021-00094-4/MediaObjects/13619_2021_94_Fig1_HTML.png
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs13619-021-00094-4/MediaObjects/13619_2021_94_Fig2_HTML.png
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs13619-021-00094-4/MediaObjects/13619_2021_94_Fig3_HTML.png
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs13619-021-00094-4/MediaObjects/13619_2021_94_Fig4_HTML.png
         isPartOf:
            name:Cell Regeneration
            issn:
               2045-9769
            volumeNumber:10
            type:
               Periodical
               PublicationVolume
         publisher:
            name:Springer Singapore
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Haonan Li
               affiliation:
                     name:Tsinghua University
                     address:
                        name:The State Key Laboratory of Membrane Biology, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Yalong Wang
               affiliation:
                     name:Tsinghua University
                     address:
                        name:The State Key Laboratory of Membrane Biology, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Mengxian Zhang
               affiliation:
                     name:Tsinghua University
                     address:
                        name:The State Key Laboratory of Membrane Biology, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Hong Wang
               affiliation:
                     name:Institute of Primate Translational Medicine, Kunming University of Science and Technology
                     address:
                        name:State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Along Cui
               affiliation:
                     name:University of Science and Technology of China
                     address:
                        name:School of Life Sciences, University of Science and Technology of China, Hefei, China
                        type:PostalAddress
                     type:Organization
                     name:Institute of Zoology, Chinese Academy of Sciences
                     address:
                        name:State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Jianguo Zhao
               affiliation:
                     name:University of Science and Technology of China
                     address:
                        name:School of Life Sciences, University of Science and Technology of China, Hefei, China
                        type:PostalAddress
                     type:Organization
                     name:Institute of Zoology, Chinese Academy of Sciences
                     address:
                        name:State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
                        type:PostalAddress
                     type:Organization
                     name:University of Chinese Academy of Sciences
                     address:
                        name:University of Chinese Academy of Sciences, Beijing, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Weizhi Ji
               affiliation:
                     name:Institute of Primate Translational Medicine, Kunming University of Science and Technology
                     address:
                        name:State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Ye-Guang Chen
               url:http://orcid.org/0000-0002-6701-0065
               affiliation:
                     name:Tsinghua University
                     address:
                        name:The State Key Laboratory of Membrane Biology, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China
                        type:PostalAddress
                     type:Organization
                     name:Guangzhou Regenerative Medicine and Health Guangdong Laboratory
                     address:
                        name:Max-Planck Center for Tissue Stem Cell Research and Regenerative Medicine, Guangzhou Regenerative Medicine and Health Guangdong Laboratory, Guangzhou, China
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
         isAccessibleForFree:1
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:Establishment of porcine and monkey colonic organoids for drug toxicity study
      description:Pig and monkey are widely used models for exploration of human diseases and evaluation of drug efficiency and toxicity, but high cost limits their uses. Organoids have been shown to be promising models for drug test as they reasonably preserve tissue structure and functions. However, colonic organoids of pig and monkey are not yet established. Here, we report a culture medium to support the growth of porcine and monkey colonic organoids. Wnt signaling and PGE2 are important for long-term expansion of the organoids, and their withdrawal results in lineage differentiation to mature cells. Furthermore, we observe that porcine colonic organoids are closer to human colonic organoids in terms of drug toxicity response. Successful establishment of porcine and monkey colonic organoids would facilitate the mechanistic investigation of the homeostatic regulation of the intestine of these animals and is useful for drug development and toxicity studies.
      datePublished:2021-10-02T00:00:00Z
      dateModified:2021-10-02T00:00:00Z
      pageStart:1
      pageEnd:10
      license:http://creativecommons.org/publicdomain/zero/1.0/
      sameAs:https://doi.org/10.1186/s13619-021-00094-4
      keywords:
         Pig
         Monkey
         Colonic organoids
         Culture
         Drug toxicity
         Cell Biology
         Stem Cells
         Biomedicine
         general
      image:
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs13619-021-00094-4/MediaObjects/13619_2021_94_Fig1_HTML.png
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs13619-021-00094-4/MediaObjects/13619_2021_94_Fig2_HTML.png
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs13619-021-00094-4/MediaObjects/13619_2021_94_Fig3_HTML.png
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs13619-021-00094-4/MediaObjects/13619_2021_94_Fig4_HTML.png
      isPartOf:
         name:Cell Regeneration
         issn:
            2045-9769
         volumeNumber:10
         type:
            Periodical
            PublicationVolume
      publisher:
         name:Springer Singapore
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Haonan Li
            affiliation:
                  name:Tsinghua University
                  address:
                     name:The State Key Laboratory of Membrane Biology, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Yalong Wang
            affiliation:
                  name:Tsinghua University
                  address:
                     name:The State Key Laboratory of Membrane Biology, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Mengxian Zhang
            affiliation:
                  name:Tsinghua University
                  address:
                     name:The State Key Laboratory of Membrane Biology, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Hong Wang
            affiliation:
                  name:Institute of Primate Translational Medicine, Kunming University of Science and Technology
                  address:
                     name:State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Along Cui
            affiliation:
                  name:University of Science and Technology of China
                  address:
                     name:School of Life Sciences, University of Science and Technology of China, Hefei, China
                     type:PostalAddress
                  type:Organization
                  name:Institute of Zoology, Chinese Academy of Sciences
                  address:
                     name:State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Jianguo Zhao
            affiliation:
                  name:University of Science and Technology of China
                  address:
                     name:School of Life Sciences, University of Science and Technology of China, Hefei, China
                     type:PostalAddress
                  type:Organization
                  name:Institute of Zoology, Chinese Academy of Sciences
                  address:
                     name:State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
                     type:PostalAddress
                  type:Organization
                  name:University of Chinese Academy of Sciences
                  address:
                     name:University of Chinese Academy of Sciences, Beijing, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Weizhi Ji
            affiliation:
                  name:Institute of Primate Translational Medicine, Kunming University of Science and Technology
                  address:
                     name:State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Ye-Guang Chen
            url:http://orcid.org/0000-0002-6701-0065
            affiliation:
                  name:Tsinghua University
                  address:
                     name:The State Key Laboratory of Membrane Biology, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China
                     type:PostalAddress
                  type:Organization
                  name:Guangzhou Regenerative Medicine and Health Guangdong Laboratory
                  address:
                     name:Max-Planck Center for Tissue Stem Cell Research and Regenerative Medicine, Guangzhou Regenerative Medicine and Health Guangdong Laboratory, Guangzhou, China
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      isAccessibleForFree:1
["Periodical","PublicationVolume"]:
      name:Cell Regeneration
      issn:
         2045-9769
      volumeNumber:10
Organization:
      name:Springer Singapore
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Tsinghua University
      address:
         name:The State Key Laboratory of Membrane Biology, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China
         type:PostalAddress
      name:Tsinghua University
      address:
         name:The State Key Laboratory of Membrane Biology, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China
         type:PostalAddress
      name:Tsinghua University
      address:
         name:The State Key Laboratory of Membrane Biology, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China
         type:PostalAddress
      name:Institute of Primate Translational Medicine, Kunming University of Science and Technology
      address:
         name:State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, China
         type:PostalAddress
      name:University of Science and Technology of China
      address:
         name:School of Life Sciences, University of Science and Technology of China, Hefei, China
         type:PostalAddress
      name:Institute of Zoology, Chinese Academy of Sciences
      address:
         name:State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
         type:PostalAddress
      name:University of Science and Technology of China
      address:
         name:School of Life Sciences, University of Science and Technology of China, Hefei, China
         type:PostalAddress
      name:Institute of Zoology, Chinese Academy of Sciences
      address:
         name:State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
         type:PostalAddress
      name:University of Chinese Academy of Sciences
      address:
         name:University of Chinese Academy of Sciences, Beijing, China
         type:PostalAddress
      name:Institute of Primate Translational Medicine, Kunming University of Science and Technology
      address:
         name:State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, China
         type:PostalAddress
      name:Tsinghua University
      address:
         name:The State Key Laboratory of Membrane Biology, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China
         type:PostalAddress
      name:Guangzhou Regenerative Medicine and Health Guangdong Laboratory
      address:
         name:Max-Planck Center for Tissue Stem Cell Research and Regenerative Medicine, Guangzhou Regenerative Medicine and Health Guangdong Laboratory, Guangzhou, China
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Haonan Li
      affiliation:
            name:Tsinghua University
            address:
               name:The State Key Laboratory of Membrane Biology, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China
               type:PostalAddress
            type:Organization
      name:Yalong Wang
      affiliation:
            name:Tsinghua University
            address:
               name:The State Key Laboratory of Membrane Biology, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China
               type:PostalAddress
            type:Organization
      name:Mengxian Zhang
      affiliation:
            name:Tsinghua University
            address:
               name:The State Key Laboratory of Membrane Biology, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China
               type:PostalAddress
            type:Organization
      name:Hong Wang
      affiliation:
            name:Institute of Primate Translational Medicine, Kunming University of Science and Technology
            address:
               name:State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, China
               type:PostalAddress
            type:Organization
      name:Along Cui
      affiliation:
            name:University of Science and Technology of China
            address:
               name:School of Life Sciences, University of Science and Technology of China, Hefei, China
               type:PostalAddress
            type:Organization
            name:Institute of Zoology, Chinese Academy of Sciences
            address:
               name:State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
               type:PostalAddress
            type:Organization
      name:Jianguo Zhao
      affiliation:
            name:University of Science and Technology of China
            address:
               name:School of Life Sciences, University of Science and Technology of China, Hefei, China
               type:PostalAddress
            type:Organization
            name:Institute of Zoology, Chinese Academy of Sciences
            address:
               name:State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
               type:PostalAddress
            type:Organization
            name:University of Chinese Academy of Sciences
            address:
               name:University of Chinese Academy of Sciences, Beijing, China
               type:PostalAddress
            type:Organization
      name:Weizhi Ji
      affiliation:
            name:Institute of Primate Translational Medicine, Kunming University of Science and Technology
            address:
               name:State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, China
               type:PostalAddress
            type:Organization
      name:Ye-Guang Chen
      url:http://orcid.org/0000-0002-6701-0065
      affiliation:
            name:Tsinghua University
            address:
               name:The State Key Laboratory of Membrane Biology, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China
               type:PostalAddress
            type:Organization
            name:Guangzhou Regenerative Medicine and Health Guangdong Laboratory
            address:
               name:Max-Planck Center for Tissue Stem Cell Research and Regenerative Medicine, Guangzhou Regenerative Medicine and Health Guangdong Laboratory, Guangzhou, China
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:The State Key Laboratory of Membrane Biology, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China
      name:The State Key Laboratory of Membrane Biology, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China
      name:The State Key Laboratory of Membrane Biology, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China
      name:State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, China
      name:School of Life Sciences, University of Science and Technology of China, Hefei, China
      name:State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
      name:School of Life Sciences, University of Science and Technology of China, Hefei, China
      name:State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
      name:University of Chinese Academy of Sciences, Beijing, China
      name:State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, China
      name:The State Key Laboratory of Membrane Biology, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China
      name:Max-Planck Center for Tissue Stem Cell Research and Regenerative Medicine, Guangzhou Regenerative Medicine and Health Guangdong Laboratory, Guangzhou, China

External Links {🔗}(198)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

CDN Services {📦}

Crossref

4.52s.

LINK . SPRINGER . COM {}