We are analyzing https://link.springer.com/article/10.1186/s13058-015-0615-y.

Title:
Multipotent luminal mammary cancer stem cells model tumor heterogeneity | Breast Cancer Research
Description:
Introduction The diversity of human breast cancer subtypes has led to the hypothesis that breast cancer is actually a number of different diseases arising from cells at various stages of differentiation. The elusive nature of the cell(s) of origin thus hampers approaches to eradicate the disease. Methods Clonal cell lines were isolated from primary transgenic polyomavirus middle T (PyVmT) luminal tumors. Mammary cancer stem cell (MaCSC) properties were examined by immunofluorescence, flow cytometry, differentiation assays and in vivo tumorigenesis. Results Clonal cell lines isolated from primary PyVmT mouse mammary luminal tumors can differentiate into luminal, myoepithelial, alveolar and adipocyte lineages. Upon orthotopic injection, progeny of a single cell follow a pattern of progression from ductal carcinoma in situ, to adenoma, adenocarcinoma and epithelial metastasis that recapitulates the transgenic model. Tumors can evolve in vivo from hormone receptor-positive to hormone receptor-negative Her2-positive, or triple negative CD44hi basal-like and claudin-low tumors. Contrary to the current paradigm, we have defined a model in which multiple tumor subtypes can originate from a single multipotent cancer stem cell that undergoes genetic and/or epigenetic evolution during tumor progression. As in human tumors, the more aggressive tumor subtypes express nuclear p53. Tumor cell lines can also be derived from these more advanced tumor subtypes. Conclusions Since the majority of human tumors are of the luminal subtype, understanding the cell of origin of these tumors and how they relate to other tumor subtypes will impact cancer therapy. Analysis of clonal cell lines derived from different tumor subtypes suggests a developmental hierarchy of MaCSCs, which may provide insights into the progression of human breast cancer.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Science
Education
Telecommunications

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
However, some sources were not loaded, we suggest to reload the page to get complete results.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We don’t know how the website earns money.

Many websites are intended to earn money, but some serve to share ideas or build connections. Websites exist for all kinds of purposes. This might be one of them. Link.springer.com has a revenue plan, but it's either invisible or we haven't found it.

Keywords {🔍}

cells, cell, tumors, luminal, mammary, cancer, breast, tumor, stem, pubmed, human, article, lines, claudinlow, expression, subtypes, google, scholar, additional, fig, clonal, usa, genes, data, line, gene, macscs, normal, file, cas, mouse, form, pyvmt, analysis, model, differentiation, macsc, figure, murine, mice, metastasis, basallike, scale, bar, vivo, single, progression, rise, sigmaaldrich, cultures,

Topics {✒️}

neighbor-weighted k-nearest neighbor cancer stem cells--perspectives hormone receptor-negative her2-positive py230-derived tumor heterogeneity article download pdf breast cancer heterogeneity pre-lactating mammary gland brca1-deficient mammary tumors cell-based model systems olympus mvx10 macro-microscope cancer stem cell claudin-low cell lines c57bl/6j strain backgrounds mammary stem cell ultra-low adhesion plates claudin-low tumors resemble cancer stem cells hormone receptor-positive tumors tumor-epithelial cell interactions rat-tail collagen gels secondary anti-rat alexa488 claudin-low macsc lines cell stem cell clonal multipotent macscs mammary stem cells claudin-low murine tumors human claudin-low tumors claudin-low cell type 10 μl 2 mg/ml matrigel py230-derived tumors representing cd24locd44hi claudin-low population pre-lactating epithelial cells tissue stem cell single-positive cells arising semi-quantitative rt-pcr real-time fluorescence measured single stem cell full access mammary fat pad form single-positive k8 significantly fewer single-positive stem cell characteristics precancer stem cell additional tumor-stromal cell double-positive single cells mammary fat pads human breast cancer breast cancer based py230-derived mesenchymal cells py230-derived domes expand

Schema {🗺️}

WebPage:
      mainEntity:
         headline:Multipotent luminal mammary cancer stem cells model tumor heterogeneity
         description:The diversity of human breast cancer subtypes has led to the hypothesis that breast cancer is actually a number of different diseases arising from cells at various stages of differentiation. The elusive nature of the cell(s) of origin thus hampers approaches to eradicate the disease. Clonal cell lines were isolated from primary transgenic polyomavirus middle T (PyVmT) luminal tumors. Mammary cancer stem cell (MaCSC) properties were examined by immunofluorescence, flow cytometry, differentiation assays and in vivo tumorigenesis. Clonal cell lines isolated from primary PyVmT mouse mammary luminal tumors can differentiate into luminal, myoepithelial, alveolar and adipocyte lineages. Upon orthotopic injection, progeny of a single cell follow a pattern of progression from ductal carcinoma in situ, to adenoma, adenocarcinoma and epithelial metastasis that recapitulates the transgenic model. Tumors can evolve in vivo from hormone receptor-positive to hormone receptor-negative Her2-positive, or triple negative CD44hi basal-like and claudin-low tumors. Contrary to the current paradigm, we have defined a model in which multiple tumor subtypes can originate from a single multipotent cancer stem cell that undergoes genetic and/or epigenetic evolution during tumor progression. As in human tumors, the more aggressive tumor subtypes express nuclear p53. Tumor cell lines can also be derived from these more advanced tumor subtypes. Since the majority of human tumors are of the luminal subtype, understanding the cell of origin of these tumors and how they relate to other tumor subtypes will impact cancer therapy. Analysis of clonal cell lines derived from different tumor subtypes suggests a developmental hierarchy of MaCSCs, which may provide insights into the progression of human breast cancer.
         datePublished:2015-10-14T00:00:00Z
         dateModified:2015-10-14T00:00:00Z
         pageStart:1
         pageEnd:14
         license:http://creativecommons.org/publicdomain/zero/1.0/
         sameAs:https://doi.org/10.1186/s13058-015-0615-y
         keywords:
            Cancer Stem Cell
            Breast Cancer Stem Cell
            Clonal Cell Line
            Mammary Stem Cell
            Luminal Tumor
            Cancer Research
            Oncology
            Surgical Oncology
         image:
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs13058-015-0615-y/MediaObjects/13058_2015_615_Fig1_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs13058-015-0615-y/MediaObjects/13058_2015_615_Fig2_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs13058-015-0615-y/MediaObjects/13058_2015_615_Fig3_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs13058-015-0615-y/MediaObjects/13058_2015_615_Fig4_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs13058-015-0615-y/MediaObjects/13058_2015_615_Fig5_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs13058-015-0615-y/MediaObjects/13058_2015_615_Fig6_HTML.gif
         isPartOf:
            name:Breast Cancer Research
            issn:
               1465-542X
            volumeNumber:17
            type:
               Periodical
               PublicationVolume
         publisher:
            name:BioMed Central
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Lei Bao
               affiliation:
                     name:University of California San Diego
                     address:
                        name:Bioinformatics and Biostatistics Shared Resource, Moores UCSD Cancer Center, University of California San Diego, La Jolla, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Robert D. Cardiff
               affiliation:
                     name:University of California
                     address:
                        name:Center for Comparative Medicine, University of California, Davis, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Paul Steinbach
               affiliation:
                     name:University of California San Diego
                     address:
                        name:Department of Pharmacology, University of California San Diego, La Jolla, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Karen S. Messer
               affiliation:
                     name:University of California San Diego
                     address:
                        name:Bioinformatics and Biostatistics Shared Resource, Moores UCSD Cancer Center, University of California San Diego, La Jolla, USA
                        type:PostalAddress
                     type:Organization
                     name:University of California San Diego
                     address:
                        name:Divison of Biostatistics and Bioinformatics, Department of Family and Preventive Medicine, University of California San Diego, La Jolla, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Lesley G. Ellies
               affiliation:
                     name:University of California San Diego
                     address:
                        name:Department of Pathology, University of California San Diego, La Jolla, USA
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
         isAccessibleForFree:1
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:Multipotent luminal mammary cancer stem cells model tumor heterogeneity
      description:The diversity of human breast cancer subtypes has led to the hypothesis that breast cancer is actually a number of different diseases arising from cells at various stages of differentiation. The elusive nature of the cell(s) of origin thus hampers approaches to eradicate the disease. Clonal cell lines were isolated from primary transgenic polyomavirus middle T (PyVmT) luminal tumors. Mammary cancer stem cell (MaCSC) properties were examined by immunofluorescence, flow cytometry, differentiation assays and in vivo tumorigenesis. Clonal cell lines isolated from primary PyVmT mouse mammary luminal tumors can differentiate into luminal, myoepithelial, alveolar and adipocyte lineages. Upon orthotopic injection, progeny of a single cell follow a pattern of progression from ductal carcinoma in situ, to adenoma, adenocarcinoma and epithelial metastasis that recapitulates the transgenic model. Tumors can evolve in vivo from hormone receptor-positive to hormone receptor-negative Her2-positive, or triple negative CD44hi basal-like and claudin-low tumors. Contrary to the current paradigm, we have defined a model in which multiple tumor subtypes can originate from a single multipotent cancer stem cell that undergoes genetic and/or epigenetic evolution during tumor progression. As in human tumors, the more aggressive tumor subtypes express nuclear p53. Tumor cell lines can also be derived from these more advanced tumor subtypes. Since the majority of human tumors are of the luminal subtype, understanding the cell of origin of these tumors and how they relate to other tumor subtypes will impact cancer therapy. Analysis of clonal cell lines derived from different tumor subtypes suggests a developmental hierarchy of MaCSCs, which may provide insights into the progression of human breast cancer.
      datePublished:2015-10-14T00:00:00Z
      dateModified:2015-10-14T00:00:00Z
      pageStart:1
      pageEnd:14
      license:http://creativecommons.org/publicdomain/zero/1.0/
      sameAs:https://doi.org/10.1186/s13058-015-0615-y
      keywords:
         Cancer Stem Cell
         Breast Cancer Stem Cell
         Clonal Cell Line
         Mammary Stem Cell
         Luminal Tumor
         Cancer Research
         Oncology
         Surgical Oncology
      image:
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs13058-015-0615-y/MediaObjects/13058_2015_615_Fig1_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs13058-015-0615-y/MediaObjects/13058_2015_615_Fig2_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs13058-015-0615-y/MediaObjects/13058_2015_615_Fig3_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs13058-015-0615-y/MediaObjects/13058_2015_615_Fig4_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs13058-015-0615-y/MediaObjects/13058_2015_615_Fig5_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs13058-015-0615-y/MediaObjects/13058_2015_615_Fig6_HTML.gif
      isPartOf:
         name:Breast Cancer Research
         issn:
            1465-542X
         volumeNumber:17
         type:
            Periodical
            PublicationVolume
      publisher:
         name:BioMed Central
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Lei Bao
            affiliation:
                  name:University of California San Diego
                  address:
                     name:Bioinformatics and Biostatistics Shared Resource, Moores UCSD Cancer Center, University of California San Diego, La Jolla, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Robert D. Cardiff
            affiliation:
                  name:University of California
                  address:
                     name:Center for Comparative Medicine, University of California, Davis, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Paul Steinbach
            affiliation:
                  name:University of California San Diego
                  address:
                     name:Department of Pharmacology, University of California San Diego, La Jolla, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Karen S. Messer
            affiliation:
                  name:University of California San Diego
                  address:
                     name:Bioinformatics and Biostatistics Shared Resource, Moores UCSD Cancer Center, University of California San Diego, La Jolla, USA
                     type:PostalAddress
                  type:Organization
                  name:University of California San Diego
                  address:
                     name:Divison of Biostatistics and Bioinformatics, Department of Family and Preventive Medicine, University of California San Diego, La Jolla, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Lesley G. Ellies
            affiliation:
                  name:University of California San Diego
                  address:
                     name:Department of Pathology, University of California San Diego, La Jolla, USA
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      isAccessibleForFree:1
["Periodical","PublicationVolume"]:
      name:Breast Cancer Research
      issn:
         1465-542X
      volumeNumber:17
Organization:
      name:BioMed Central
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:University of California San Diego
      address:
         name:Bioinformatics and Biostatistics Shared Resource, Moores UCSD Cancer Center, University of California San Diego, La Jolla, USA
         type:PostalAddress
      name:University of California
      address:
         name:Center for Comparative Medicine, University of California, Davis, USA
         type:PostalAddress
      name:University of California San Diego
      address:
         name:Department of Pharmacology, University of California San Diego, La Jolla, USA
         type:PostalAddress
      name:University of California San Diego
      address:
         name:Bioinformatics and Biostatistics Shared Resource, Moores UCSD Cancer Center, University of California San Diego, La Jolla, USA
         type:PostalAddress
      name:University of California San Diego
      address:
         name:Divison of Biostatistics and Bioinformatics, Department of Family and Preventive Medicine, University of California San Diego, La Jolla, USA
         type:PostalAddress
      name:University of California San Diego
      address:
         name:Department of Pathology, University of California San Diego, La Jolla, USA
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Lei Bao
      affiliation:
            name:University of California San Diego
            address:
               name:Bioinformatics and Biostatistics Shared Resource, Moores UCSD Cancer Center, University of California San Diego, La Jolla, USA
               type:PostalAddress
            type:Organization
      name:Robert D. Cardiff
      affiliation:
            name:University of California
            address:
               name:Center for Comparative Medicine, University of California, Davis, USA
               type:PostalAddress
            type:Organization
      name:Paul Steinbach
      affiliation:
            name:University of California San Diego
            address:
               name:Department of Pharmacology, University of California San Diego, La Jolla, USA
               type:PostalAddress
            type:Organization
      name:Karen S. Messer
      affiliation:
            name:University of California San Diego
            address:
               name:Bioinformatics and Biostatistics Shared Resource, Moores UCSD Cancer Center, University of California San Diego, La Jolla, USA
               type:PostalAddress
            type:Organization
            name:University of California San Diego
            address:
               name:Divison of Biostatistics and Bioinformatics, Department of Family and Preventive Medicine, University of California San Diego, La Jolla, USA
               type:PostalAddress
            type:Organization
      name:Lesley G. Ellies
      affiliation:
            name:University of California San Diego
            address:
               name:Department of Pathology, University of California San Diego, La Jolla, USA
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Bioinformatics and Biostatistics Shared Resource, Moores UCSD Cancer Center, University of California San Diego, La Jolla, USA
      name:Center for Comparative Medicine, University of California, Davis, USA
      name:Department of Pharmacology, University of California San Diego, La Jolla, USA
      name:Bioinformatics and Biostatistics Shared Resource, Moores UCSD Cancer Center, University of California San Diego, La Jolla, USA
      name:Divison of Biostatistics and Bioinformatics, Department of Family and Preventive Medicine, University of California San Diego, La Jolla, USA
      name:Department of Pathology, University of California San Diego, La Jolla, USA

External Links {🔗}(147)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

CDN Services {📦}

Crossref

4.61s.

LINK . SPRINGER . COM {}