We are analyzing https://link.springer.com/article/10.1186/s12967-016-0983-9.

Title:
Differential tumor infiltration by T-cells characterizes intrinsic molecular subtypes in breast cancer | Journal of Translational Medicine
Description:
Molecular subtypes of breast cancer and presence of tumor-infiltrating immune cells have both been implicated as important predictive and prognostic factors for improved risk stratification and treatment individualization of breast cancer patients. Their association, however, has not been studied in detail. The aim of this study was to evaluate the expression of the T cell markers CD8, FoxP3, CD3 and ζ-chain in molecular subtypes of the invasive margin and tumor center of breast cancer and corresponding sentinel nodes and to deduct prognostic information from these findings. Tumor and sentinel node sections from 177 patients with primary, invasive, unilateral early-stage breast cancer were stained by immunohistochemistry and T-cell phenotypes quantified manually. Clinical data were collected from medical records. The degree of T-cell infiltration and expression of all markers differed significantly among the molecular subtypes, being highest in non-luminal, more aggressive tumors: more T-cell infiltration and higher expression of all markers were associated with hormone receptor negativity, higher proliferation and higher histological grades, but also with larger tumor size. Basal-like tumors, and most remarkably their tumor centers, hosted the highest number of FoxP3+ T-cells with an unfavorable ratio to cytotoxic CD8+ T-cells. T-cell infiltration was generally higher in the invasive margin than the tumor center. A scoring system based on densities of CD3 and CD8 could significantly separate molecular subtypes (p < 0.001). Thus, immunological patterns with functional implications within each subtype are associated with prognostic factors. These findings should be further validated in studies using larger patient populations and longer follow-up.
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Keywords {🔍}

cancer, tumor, subtypes, breast, pubmed, article, tumors, molecular, tcell, google, scholar, tcells, patients, foxp, luminal, center, prognostic, cas, subtype, infiltration, immune, higher, immunological, cells, invasive, min, markers, margin, phenotypes, density, study, densities, analysis, clinical, significantly, data, expression, response, high, sections, nonluminal, basallike, ratio, lymphocytes, sentinel, lhr, samples, central, number, treatment,

Topics {✒️}

ζ-chain/cd3-ratio positively correlated her2/neu-overexpressing breast cancer cd8+ t-cells markedly differed tumor-infiltrating cd8+ t-cells article download pdf cytotoxic t-cell responses detect pd-l1 expression recruit regulatory t-cells chi square cross-tabulation foxp3+ tumor-infiltrating lymphocytes intrastromal t-cell trapping t-cell phenotypes differs paraffin-embedded tumor blocks cytotoxic cd8+ t-cells densest t-cell infiltration refrigerator-tempered moisture chamber main t-cell area t-cell functionality differs her2/neu-positive subtype her2/neu positive tumors compare long-term survival tumor-infiltrating immune cells immunological tumor-host interactions tumour infiltrating lymphocytes tumor-infiltrating lymphocytes tumor infiltrating lymphocytes her2/neu overexpressing tumors studies found pd-l1 analyzed t-cell phenotypes �tumor-observing’ immune response ζ-chain+ t-cells high t-cell densities luminal her2/neu-overexpressing luminal her2/neu overexpressing her2-negative breast cancer biotinylated horse-anti-mouse higher ζ-chain/cd3 ratio study t-cell infiltration regulatory t-cells water-based assemblage material naturally occurring regulatory her2 positive-subtype compared cytotoxic t-cells full access t-cell receptor node-negative breast cancer xylene-based assemblage-material fewer inter-subtype differences lymphocytic host response her2/neu-status

Questions {❓}

Down-regulation of zeta-chain expression in T cells: a biomarker of prognosis in cancer?

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WebPage:
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         headline:Differential tumor infiltration by T-cells characterizes intrinsic molecular subtypes in breast cancer
         description:Molecular subtypes of breast cancer and presence of tumor-infiltrating immune cells have both been implicated as important predictive and prognostic factors for improved risk stratification and treatment individualization of breast cancer patients. Their association, however, has not been studied in detail. The aim of this study was to evaluate the expression of the T cell markers CD8, FoxP3, CD3 and ζ-chain in molecular subtypes of the invasive margin and tumor center of breast cancer and corresponding sentinel nodes and to deduct prognostic information from these findings. Tumor and sentinel node sections from 177 patients with primary, invasive, unilateral early-stage breast cancer were stained by immunohistochemistry and T-cell phenotypes quantified manually. Clinical data were collected from medical records. The degree of T-cell infiltration and expression of all markers differed significantly among the molecular subtypes, being highest in non-luminal, more aggressive tumors: more T-cell infiltration and higher expression of all markers were associated with hormone receptor negativity, higher proliferation and higher histological grades, but also with larger tumor size. Basal-like tumors, and most remarkably their tumor centers, hosted the highest number of FoxP3+ T-cells with an unfavorable ratio to cytotoxic CD8+ T-cells. T-cell infiltration was generally higher in the invasive margin than the tumor center. A scoring system based on densities of CD3 and CD8 could significantly separate molecular subtypes (p < 0.001). Thus, immunological patterns with functional implications within each subtype are associated with prognostic factors. These findings should be further validated in studies using larger patient populations and longer follow-up.
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      description:Molecular subtypes of breast cancer and presence of tumor-infiltrating immune cells have both been implicated as important predictive and prognostic factors for improved risk stratification and treatment individualization of breast cancer patients. Their association, however, has not been studied in detail. The aim of this study was to evaluate the expression of the T cell markers CD8, FoxP3, CD3 and ζ-chain in molecular subtypes of the invasive margin and tumor center of breast cancer and corresponding sentinel nodes and to deduct prognostic information from these findings. Tumor and sentinel node sections from 177 patients with primary, invasive, unilateral early-stage breast cancer were stained by immunohistochemistry and T-cell phenotypes quantified manually. Clinical data were collected from medical records. The degree of T-cell infiltration and expression of all markers differed significantly among the molecular subtypes, being highest in non-luminal, more aggressive tumors: more T-cell infiltration and higher expression of all markers were associated with hormone receptor negativity, higher proliferation and higher histological grades, but also with larger tumor size. Basal-like tumors, and most remarkably their tumor centers, hosted the highest number of FoxP3+ T-cells with an unfavorable ratio to cytotoxic CD8+ T-cells. T-cell infiltration was generally higher in the invasive margin than the tumor center. A scoring system based on densities of CD3 and CD8 could significantly separate molecular subtypes (p < 0.001). Thus, immunological patterns with functional implications within each subtype are associated with prognostic factors. These findings should be further validated in studies using larger patient populations and longer follow-up.
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