We are analyzing https://link.springer.com/article/10.1186/s12885-018-4504-5.

Title:
Resveratrol induces autophagy-dependent apoptosis in HL-60 cells | BMC Cancer
Description:
Background All known mechanisms of apoptosis induced by resveratrol act through cell cycle arrest and changes in mitochondrial membrane potential. It is currently unknown whether resveratrol-induced apoptosis is associated with other physiological processes, such as autophagy. Methods Apoptosis-related markers involved in the intrinsic and extrinsic apoptotic pathways, and autophagic markers were detected by using western blotting and immunofluorescence. Mitochondrial membrane potential was assayed by flow cytometry. Pharmaceutical or genetic inhibition of autophagy involved were carried by 3- methyladenine or knockdown of autophagy-related (Atg) genes by siRNA. Differences between two values were tested by Student’s unpaired t test. Results We show that resveratrol-induced apoptosis occurs through both the intrinsic and extrinsic apoptotic pathways. Mitochondrial membrane potential and apoptosis-related markers, such as an increased Bax/Bcl-2 ratio, and cleaved forms of caspase-8 and caspase-3, arise following resveratrol addition. Moreover, we find that resveratrol increases both the levels of microtubule-associated protein 1 light chain 3-II and the number of autophagosomes, and further demonstrate that resveratrol-induced autophagy depends on the LKB1-AMPK-mTOR pathway. We next reveal that some apoptosis-related markers induced by resveratrol are further attenuated by the inhibition of autophagy with 3-methyladenine or knockdown of autophagy-related (Atg) genes by siRNA. Conclusions These results suggest that resveratrol induced apoptotic cell death of HL-60 cells depends on the autophagy activated through both the LKB1-AMPK and PI3K/AKT-regulated mTOR signaling pathways.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Science
Education
Telecommunications

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
However, some sources were not loaded, we suggest to reload the page to get complete results.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We don’t know how the website earns money.

While many websites aim to make money, others are created to share knowledge or showcase creativity. People build websites for various reasons. This could be one of them. Link.springer.com could be getting rich in stealth mode, or the way it's monetizing isn't detectable.

Keywords {🔍}

rsv, cells, cell, apoptosis, autophagy, article, death, caspase, pubmed, scholar, treated, google, resveratrol, cas, data, fig, induces, analysis, cancer, pathway, control, treatment, induced, western, inhibition, mtor, blot, times, pathways, time, intrinsic, results, analyzed, study, expression, rsvinduced, viability, concentrations, mitochondrial, involved, increased, protein, activation, experiments, activity, zhang, manner, extrinsic, apoptotic, cleaved,

Topics {✒️}

caspase-3 inhibitor ac-devd-cho ice-cold phosphate-buffered saline caspase inhibitor z-devd-fmk 4′-trihydroxy-trans-stilbene induces apoptosis jnk-mediated p62/sqstm1 expression annexin v-pi double-staining anti-rabbit-secondary antibody sequestosome1 z-devd-fmk fmk z-ietd-fmk pi3k/akt/mtor/p70s6k inhibition anti-mouse-secondary antibody pi3k-akt signaling contribute rsv-mediated cell death cleavage fragment t-bid 10 μm z-devd-fmk rsv-induced cell death article download pdf micro-plate auto-reader resveratrol-induced apoptosis occurs endoplasmic reticulum stress 100 μl sds-triplex liquid z-devd-fmk tumor molecular biology/department induces cell death resveratrol-induced autophagy depends autophagic cell death human promyelocytic leukemia lkb1-ampk-mtor pathways od treatment/od control apoptosis-related markers induced lkb1-ampk-mtor pathway van woensel jb programmed cell death 50 mm tris–hcl ph 7 pi3k/akt-mtor cell signaling technology triggers cell death enabling cell death cell death rate cell death differ cell death dis pi3k-akt pathways febs open bio blocking autophagosome formation pi3k-akt pathway privacy choices/manage cookies ros-triggered autophagy respective control cells autophagy-related marker lc3 resveratrol induces apoptosis

Schema {🗺️}

WebPage:
      mainEntity:
         headline:Resveratrol induces autophagy-dependent apoptosis in HL-60 cells
         description:All known mechanisms of apoptosis induced by resveratrol act through cell cycle arrest and changes in mitochondrial membrane potential. It is currently unknown whether resveratrol-induced apoptosis is associated with other physiological processes, such as autophagy. Apoptosis-related markers involved in the intrinsic and extrinsic apoptotic pathways, and autophagic markers were detected by using western blotting and immunofluorescence. Mitochondrial membrane potential was assayed by flow cytometry. Pharmaceutical or genetic inhibition of autophagy involved were carried by 3- methyladenine or knockdown of autophagy-related (Atg) genes by siRNA. Differences between two values were tested by Student’s unpaired t test. We show that resveratrol-induced apoptosis occurs through both the intrinsic and extrinsic apoptotic pathways. Mitochondrial membrane potential and apoptosis-related markers, such as an increased Bax/Bcl-2 ratio, and cleaved forms of caspase-8 and caspase-3, arise following resveratrol addition. Moreover, we find that resveratrol increases both the levels of microtubule-associated protein 1 light chain 3-II and the number of autophagosomes, and further demonstrate that resveratrol-induced autophagy depends on the LKB1-AMPK-mTOR pathway. We next reveal that some apoptosis-related markers induced by resveratrol are further attenuated by the inhibition of autophagy with 3-methyladenine or knockdown of autophagy-related (Atg) genes by siRNA. These results suggest that resveratrol induced apoptotic cell death of HL-60 cells depends on the autophagy activated through both the LKB1-AMPK and PI3K/AKT-regulated mTOR signaling pathways.
         datePublished:2018-05-22T00:00:00Z
         dateModified:2018-05-22T00:00:00Z
         pageStart:1
         pageEnd:10
         license:http://creativecommons.org/publicdomain/zero/1.0/
         sameAs:https://doi.org/10.1186/s12885-018-4504-5
         keywords:
            Resveratrol
            Apoptosis
            Autophagy
            Cell death
            PI3K-Akt
            AMPK-mTOR
            HL-60
            Cancer Research
            Oncology
            Surgical Oncology
            Health Promotion and Disease Prevention
            Biomedicine
            general
            Medicine/Public Health
         image:
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs12885-018-4504-5/MediaObjects/12885_2018_4504_Fig1_HTML.png
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs12885-018-4504-5/MediaObjects/12885_2018_4504_Fig2_HTML.png
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs12885-018-4504-5/MediaObjects/12885_2018_4504_Fig3_HTML.png
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs12885-018-4504-5/MediaObjects/12885_2018_4504_Fig4_HTML.png
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs12885-018-4504-5/MediaObjects/12885_2018_4504_Fig5_HTML.png
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs12885-018-4504-5/MediaObjects/12885_2018_4504_Fig6_HTML.png
         isPartOf:
            name:BMC Cancer
            issn:
               1471-2407
            volumeNumber:18
            type:
               Periodical
               PublicationVolume
         publisher:
            name:BioMed Central
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Yingying Fan
               affiliation:
                     name:Shantou University Medical College
                     address:
                        name:Open Laboratory for Tumor Molecular Biology/Department of Biochemistry/The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Jen-Fu Chiu
               affiliation:
                     name:Shantou University Medical College
                     address:
                        name:Open Laboratory for Tumor Molecular Biology/Department of Biochemistry/The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Jing Liu
               affiliation:
                     name:Shantou University Medical College
                     address:
                        name:Cheung Kong Scholar Laboratory, Shantou University Medical College, Shantou, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Yan Deng
               affiliation:
                     name:Shantou University Medical College
                     address:
                        name:Open Laboratory for Tumor Molecular Biology/Department of Biochemistry/The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, China
                        type:PostalAddress
                     type:Organization
                     name:The first Affiliated Hospital of Shantou University Medical College
                     address:
                        name:Respiratory Department, The first Affiliated Hospital of Shantou University Medical College, Shantou, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Cheng Xu
               affiliation:
                     name:Shantou University Medical College
                     address:
                        name:Open Laboratory for Tumor Molecular Biology/Department of Biochemistry/The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Jun Zhang
               affiliation:
                     name:Shantou University Medical College
                     address:
                        name:Open Laboratory for Tumor Molecular Biology/Department of Biochemistry/The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Guanwu Li
               url:http://orcid.org/0000-0003-3133-4460
               affiliation:
                     name:Shantou University Medical College
                     address:
                        name:Open Laboratory for Tumor Molecular Biology/Department of Biochemistry/The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, China
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
         isAccessibleForFree:1
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:Resveratrol induces autophagy-dependent apoptosis in HL-60 cells
      description:All known mechanisms of apoptosis induced by resveratrol act through cell cycle arrest and changes in mitochondrial membrane potential. It is currently unknown whether resveratrol-induced apoptosis is associated with other physiological processes, such as autophagy. Apoptosis-related markers involved in the intrinsic and extrinsic apoptotic pathways, and autophagic markers were detected by using western blotting and immunofluorescence. Mitochondrial membrane potential was assayed by flow cytometry. Pharmaceutical or genetic inhibition of autophagy involved were carried by 3- methyladenine or knockdown of autophagy-related (Atg) genes by siRNA. Differences between two values were tested by Student’s unpaired t test. We show that resveratrol-induced apoptosis occurs through both the intrinsic and extrinsic apoptotic pathways. Mitochondrial membrane potential and apoptosis-related markers, such as an increased Bax/Bcl-2 ratio, and cleaved forms of caspase-8 and caspase-3, arise following resveratrol addition. Moreover, we find that resveratrol increases both the levels of microtubule-associated protein 1 light chain 3-II and the number of autophagosomes, and further demonstrate that resveratrol-induced autophagy depends on the LKB1-AMPK-mTOR pathway. We next reveal that some apoptosis-related markers induced by resveratrol are further attenuated by the inhibition of autophagy with 3-methyladenine or knockdown of autophagy-related (Atg) genes by siRNA. These results suggest that resveratrol induced apoptotic cell death of HL-60 cells depends on the autophagy activated through both the LKB1-AMPK and PI3K/AKT-regulated mTOR signaling pathways.
      datePublished:2018-05-22T00:00:00Z
      dateModified:2018-05-22T00:00:00Z
      pageStart:1
      pageEnd:10
      license:http://creativecommons.org/publicdomain/zero/1.0/
      sameAs:https://doi.org/10.1186/s12885-018-4504-5
      keywords:
         Resveratrol
         Apoptosis
         Autophagy
         Cell death
         PI3K-Akt
         AMPK-mTOR
         HL-60
         Cancer Research
         Oncology
         Surgical Oncology
         Health Promotion and Disease Prevention
         Biomedicine
         general
         Medicine/Public Health
      image:
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs12885-018-4504-5/MediaObjects/12885_2018_4504_Fig1_HTML.png
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs12885-018-4504-5/MediaObjects/12885_2018_4504_Fig2_HTML.png
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs12885-018-4504-5/MediaObjects/12885_2018_4504_Fig3_HTML.png
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs12885-018-4504-5/MediaObjects/12885_2018_4504_Fig4_HTML.png
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs12885-018-4504-5/MediaObjects/12885_2018_4504_Fig5_HTML.png
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs12885-018-4504-5/MediaObjects/12885_2018_4504_Fig6_HTML.png
      isPartOf:
         name:BMC Cancer
         issn:
            1471-2407
         volumeNumber:18
         type:
            Periodical
            PublicationVolume
      publisher:
         name:BioMed Central
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Yingying Fan
            affiliation:
                  name:Shantou University Medical College
                  address:
                     name:Open Laboratory for Tumor Molecular Biology/Department of Biochemistry/The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Jen-Fu Chiu
            affiliation:
                  name:Shantou University Medical College
                  address:
                     name:Open Laboratory for Tumor Molecular Biology/Department of Biochemistry/The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Jing Liu
            affiliation:
                  name:Shantou University Medical College
                  address:
                     name:Cheung Kong Scholar Laboratory, Shantou University Medical College, Shantou, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Yan Deng
            affiliation:
                  name:Shantou University Medical College
                  address:
                     name:Open Laboratory for Tumor Molecular Biology/Department of Biochemistry/The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, China
                     type:PostalAddress
                  type:Organization
                  name:The first Affiliated Hospital of Shantou University Medical College
                  address:
                     name:Respiratory Department, The first Affiliated Hospital of Shantou University Medical College, Shantou, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Cheng Xu
            affiliation:
                  name:Shantou University Medical College
                  address:
                     name:Open Laboratory for Tumor Molecular Biology/Department of Biochemistry/The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Jun Zhang
            affiliation:
                  name:Shantou University Medical College
                  address:
                     name:Open Laboratory for Tumor Molecular Biology/Department of Biochemistry/The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Guanwu Li
            url:http://orcid.org/0000-0003-3133-4460
            affiliation:
                  name:Shantou University Medical College
                  address:
                     name:Open Laboratory for Tumor Molecular Biology/Department of Biochemistry/The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, China
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      isAccessibleForFree:1
["Periodical","PublicationVolume"]:
      name:BMC Cancer
      issn:
         1471-2407
      volumeNumber:18
Organization:
      name:BioMed Central
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Shantou University Medical College
      address:
         name:Open Laboratory for Tumor Molecular Biology/Department of Biochemistry/The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, China
         type:PostalAddress
      name:Shantou University Medical College
      address:
         name:Open Laboratory for Tumor Molecular Biology/Department of Biochemistry/The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, China
         type:PostalAddress
      name:Shantou University Medical College
      address:
         name:Cheung Kong Scholar Laboratory, Shantou University Medical College, Shantou, China
         type:PostalAddress
      name:Shantou University Medical College
      address:
         name:Open Laboratory for Tumor Molecular Biology/Department of Biochemistry/The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, China
         type:PostalAddress
      name:The first Affiliated Hospital of Shantou University Medical College
      address:
         name:Respiratory Department, The first Affiliated Hospital of Shantou University Medical College, Shantou, China
         type:PostalAddress
      name:Shantou University Medical College
      address:
         name:Open Laboratory for Tumor Molecular Biology/Department of Biochemistry/The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, China
         type:PostalAddress
      name:Shantou University Medical College
      address:
         name:Open Laboratory for Tumor Molecular Biology/Department of Biochemistry/The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, China
         type:PostalAddress
      name:Shantou University Medical College
      address:
         name:Open Laboratory for Tumor Molecular Biology/Department of Biochemistry/The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, China
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Yingying Fan
      affiliation:
            name:Shantou University Medical College
            address:
               name:Open Laboratory for Tumor Molecular Biology/Department of Biochemistry/The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, China
               type:PostalAddress
            type:Organization
      name:Jen-Fu Chiu
      affiliation:
            name:Shantou University Medical College
            address:
               name:Open Laboratory for Tumor Molecular Biology/Department of Biochemistry/The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, China
               type:PostalAddress
            type:Organization
      name:Jing Liu
      affiliation:
            name:Shantou University Medical College
            address:
               name:Cheung Kong Scholar Laboratory, Shantou University Medical College, Shantou, China
               type:PostalAddress
            type:Organization
      name:Yan Deng
      affiliation:
            name:Shantou University Medical College
            address:
               name:Open Laboratory for Tumor Molecular Biology/Department of Biochemistry/The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, China
               type:PostalAddress
            type:Organization
            name:The first Affiliated Hospital of Shantou University Medical College
            address:
               name:Respiratory Department, The first Affiliated Hospital of Shantou University Medical College, Shantou, China
               type:PostalAddress
            type:Organization
      name:Cheng Xu
      affiliation:
            name:Shantou University Medical College
            address:
               name:Open Laboratory for Tumor Molecular Biology/Department of Biochemistry/The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, China
               type:PostalAddress
            type:Organization
      name:Jun Zhang
      affiliation:
            name:Shantou University Medical College
            address:
               name:Open Laboratory for Tumor Molecular Biology/Department of Biochemistry/The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, China
               type:PostalAddress
            type:Organization
      name:Guanwu Li
      url:http://orcid.org/0000-0003-3133-4460
      affiliation:
            name:Shantou University Medical College
            address:
               name:Open Laboratory for Tumor Molecular Biology/Department of Biochemistry/The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, China
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Open Laboratory for Tumor Molecular Biology/Department of Biochemistry/The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, China
      name:Open Laboratory for Tumor Molecular Biology/Department of Biochemistry/The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, China
      name:Cheung Kong Scholar Laboratory, Shantou University Medical College, Shantou, China
      name:Open Laboratory for Tumor Molecular Biology/Department of Biochemistry/The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, China
      name:Respiratory Department, The first Affiliated Hospital of Shantou University Medical College, Shantou, China
      name:Open Laboratory for Tumor Molecular Biology/Department of Biochemistry/The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, China
      name:Open Laboratory for Tumor Molecular Biology/Department of Biochemistry/The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, China
      name:Open Laboratory for Tumor Molecular Biology/Department of Biochemistry/The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, China

External Links {🔗}(145)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

CDN Services {📦}

Crossref

4.26s.

LINK . SPRINGER . COM {}