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  2. Matching Content Categories
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  4. Monthly Traffic Estimate
  5. How Does Link.springer.com Make Money
  6. Keywords
  7. Topics
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We are analyzing https://link.springer.com/article/10.1186/s12885-015-1291-0.

Title:
Curcumin potentiates antitumor activity of 5-fluorouracil in a 3D alginate tumor microenvironment of colorectal cancer | BMC Cancer
Description:
Background To overcome the limitations of animal-based experiments, 3D culture models mimicking the tumor microenvironment in vivo are gaining attention. Herein, we investigated an alginate-based 3D scaffold for screening of 5-fluorouracil (5-FU) or/and curcumin on malignancy of colorectal cancer cells (CRC). Methods The potentiation effects of curcumin on 5-FU against proliferation and metastasis of HCT116 cell and its corresponding isogenic 5-FU-chemoresistant cells (HCT116R) were examined in a 3D-alginate tumor model. Results CRC cells encapsulated in alginate were able to proliferate in 3D-colonospheres in a vivo-like phenotype and invaded from alginate. During cultivation of cells in alginate, we could isolate 3 stages of cells, (1) alginate proliferating (2) invasive and (3) adherent cells. Tumor-promoting factors (CXCR4, MMP-9, NF-κB) were significantly increased in the proliferating and invasive compared to the adherent cells, however HCT116R cells overexpressed factors in comparison to the parental HCT116, suggesting an increase in malignancy behavior. In alginate, curcumin potentiated 5-FU-induced decreased capacity for proliferation, invasion and increased more sensitivity to 5-FU of HCT116R compared to the HCT116 cells. IC50 for HCT116 to 5-FU was 8nM, but co-treatment with 5 μM curcumin significantly reduced 5-FU concentrations in HCT116 and HCT116R cells (0.8nM, 0.1nM, respectively) and these effects were accompanied by down-regulation of NF-κB activation and NF-κB-regulated gene products. Conclusions Our results demonstrate that the alginate provides an excellent tumor microenvironment and indicate that curcumin potentiates and chemosensitizes HCT116R cells to 5-FU-based chemotherapy that may be useful for the treatment of CRC and to overcome drug resistance.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

  • Science
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Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

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Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month


Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
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How Does Link.springer.com Make Money? {💸}

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Keywords {🔍}

cells, curcumin, alginate, hct, hctr, cell, cancer, pubmed, article, google, scholar, beads, tumor, cas, culture, figure, crc, days, proliferation, nfκb, treatment, expression, metastasis, cultures, colorectal, invasion, microenvironment, results, vitro, effect, shown, inhibition, effects, medium, combination, values, viability, significantly, compared, activation, growth, experiments, vivo, colon, concentration, resistant, central, increased, concentrations, gene,

Topics {✒️}

nf-κb-regulated gene products acknowledge ludwig-maximilian-university munich nuclear factor-kappab pathway article download pdf alginate-based 3d scaffold phase-contrast microscopic observations pre-testing clinical treatments nf-κb signaling pathway specific antigen-antibody complexes specific β-actin antibody greater anti-tumor activity minimizing animal-based experiments ludwig-maximilian-university munich transcription factor nf-kappa chitosan-alginate 3d scaffolds phase-contrast microscopic evaluation open access license nf-κb transcription factor nuclear factor-kappab p65 3d-alginate tumor model regulates nf-κb pathway typical spherical shape colon cancer stem nik/ikk signalling complex anti-phospho-specific p65 curcumin mediates chemosensitization colorectal cancer research suppress nf-κb activation vitro cell-based experiments spherical alginate-beads culture healthy human subjects enhanced anti-tumor therapy human breast cancer anticancer drug-induced activation potentiated 5-fu-induced inhibition nuclear factor-kappa extracellular matrix compounds extracellular matrix topology isogenic 5-fu-chemoresistant cells metastatic gene products human colorectal tumor baylor research institute nuclear factor-kappab wilson 3rd dm significantly inhibited nf-κb support curcumin-mediated inhibition phosphate buffered saline vitro pre-testing block nf-κb activation privacy choices/manage cookies

Questions {❓}

  • How does the extracellular matrix direct gene expression?
  • Is there a role for chemotherapy in prostate cancer?

Schema {🗺️}

WebPage:
      mainEntity:
         headline:Curcumin potentiates antitumor activity of 5-fluorouracil in a 3D alginate tumor microenvironment of colorectal cancer
         description:To overcome the limitations of animal-based experiments, 3D culture models mimicking the tumor microenvironment in vivo are gaining attention. Herein, we investigated an alginate-based 3D scaffold for screening of 5-fluorouracil (5-FU) or/and curcumin on malignancy of colorectal cancer cells (CRC). The potentiation effects of curcumin on 5-FU against proliferation and metastasis of HCT116 cell and its corresponding isogenic 5-FU-chemoresistant cells (HCT116R) were examined in a 3D-alginate tumor model. CRC cells encapsulated in alginate were able to proliferate in 3D-colonospheres in a vivo-like phenotype and invaded from alginate. During cultivation of cells in alginate, we could isolate 3 stages of cells, (1) alginate proliferating (2) invasive and (3) adherent cells. Tumor-promoting factors (CXCR4, MMP-9, NF-κB) were significantly increased in the proliferating and invasive compared to the adherent cells, however HCT116R cells overexpressed factors in comparison to the parental HCT116, suggesting an increase in malignancy behavior. In alginate, curcumin potentiated 5-FU-induced decreased capacity for proliferation, invasion and increased more sensitivity to 5-FU of HCT116R compared to the HCT116 cells. IC50 for HCT116 to 5-FU was 8nM, but co-treatment with 5 μM curcumin significantly reduced 5-FU concentrations in HCT116 and HCT116R cells (0.8nM, 0.1nM, respectively) and these effects were accompanied by down-regulation of NF-κB activation and NF-κB-regulated gene products. Our results demonstrate that the alginate provides an excellent tumor microenvironment and indicate that curcumin potentiates and chemosensitizes HCT116R cells to 5-FU-based chemotherapy that may be useful for the treatment of CRC and to overcome drug resistance.
         datePublished:2015-04-10T00:00:00Z
         dateModified:2015-04-10T00:00:00Z
         pageStart:1
         pageEnd:15
         license:http://creativecommons.org/publicdomain/zero/1.0/
         sameAs:https://doi.org/10.1186/s12885-015-1291-0
         keywords:
            Human colon cancer
            Alginate
            Metastasis
            Curcumin
            5-FU
            Chemosensitization
            Cancer Research
            Oncology
            Surgical Oncology
            Health Promotion and Disease Prevention
            Biomedicine
            general
            Medicine/Public Health
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               name:Patricia Kraehe
               affiliation:
                     name:Ludwig-Maximilian-University Munich
                     address:
                        name:Institute of Anatomy, Ludwig-Maximilian-University Munich, Munich, Germany
                        type:PostalAddress
                     type:Organization
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               name:Bastian Popper
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                     name:Ludwig-Maximilian-University Munich
                     address:
                        name:Department of Anatomy and Cell Biology, Ludwig-Maximilian-University Munich, Munich, Germany
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Parviz Shayan
               affiliation:
                     name:Investigating Institute of Molecular Biological System Transfer
                     address:
                        name:Investigating Institute of Molecular Biological System Transfer, Tehran, Iran
                        type:PostalAddress
                     type:Organization
                     name:University of Tehran
                     address:
                        name:Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
                        type:PostalAddress
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               affiliation:
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                     address:
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                     address:
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ScholarlyArticle:
      headline:Curcumin potentiates antitumor activity of 5-fluorouracil in a 3D alginate tumor microenvironment of colorectal cancer
      description:To overcome the limitations of animal-based experiments, 3D culture models mimicking the tumor microenvironment in vivo are gaining attention. Herein, we investigated an alginate-based 3D scaffold for screening of 5-fluorouracil (5-FU) or/and curcumin on malignancy of colorectal cancer cells (CRC). The potentiation effects of curcumin on 5-FU against proliferation and metastasis of HCT116 cell and its corresponding isogenic 5-FU-chemoresistant cells (HCT116R) were examined in a 3D-alginate tumor model. CRC cells encapsulated in alginate were able to proliferate in 3D-colonospheres in a vivo-like phenotype and invaded from alginate. During cultivation of cells in alginate, we could isolate 3 stages of cells, (1) alginate proliferating (2) invasive and (3) adherent cells. Tumor-promoting factors (CXCR4, MMP-9, NF-κB) were significantly increased in the proliferating and invasive compared to the adherent cells, however HCT116R cells overexpressed factors in comparison to the parental HCT116, suggesting an increase in malignancy behavior. In alginate, curcumin potentiated 5-FU-induced decreased capacity for proliferation, invasion and increased more sensitivity to 5-FU of HCT116R compared to the HCT116 cells. IC50 for HCT116 to 5-FU was 8nM, but co-treatment with 5 μM curcumin significantly reduced 5-FU concentrations in HCT116 and HCT116R cells (0.8nM, 0.1nM, respectively) and these effects were accompanied by down-regulation of NF-κB activation and NF-κB-regulated gene products. Our results demonstrate that the alginate provides an excellent tumor microenvironment and indicate that curcumin potentiates and chemosensitizes HCT116R cells to 5-FU-based chemotherapy that may be useful for the treatment of CRC and to overcome drug resistance.
      datePublished:2015-04-10T00:00:00Z
      dateModified:2015-04-10T00:00:00Z
      pageStart:1
      pageEnd:15
      license:http://creativecommons.org/publicdomain/zero/1.0/
      sameAs:https://doi.org/10.1186/s12885-015-1291-0
      keywords:
         Human colon cancer
         Alginate
         Metastasis
         Curcumin
         5-FU
         Chemosensitization
         Cancer Research
         Oncology
         Surgical Oncology
         Health Promotion and Disease Prevention
         Biomedicine
         general
         Medicine/Public Health
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         name:BMC Cancer
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         volumeNumber:15
         type:
            Periodical
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      publisher:
         name:BioMed Central
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Mehdi Shakibaei
            affiliation:
                  name:Ludwig-Maximilian-University Munich
                  address:
                     name:Institute of Anatomy, Ludwig-Maximilian-University Munich, Munich, Germany
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
            name:Patricia Kraehe
            affiliation:
                  name:Ludwig-Maximilian-University Munich
                  address:
                     name:Institute of Anatomy, Ludwig-Maximilian-University Munich, Munich, Germany
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Bastian Popper
            affiliation:
                  name:Ludwig-Maximilian-University Munich
                  address:
                     name:Department of Anatomy and Cell Biology, Ludwig-Maximilian-University Munich, Munich, Germany
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Parviz Shayan
            affiliation:
                  name:Investigating Institute of Molecular Biological System Transfer
                  address:
                     name:Investigating Institute of Molecular Biological System Transfer, Tehran, Iran
                     type:PostalAddress
                  type:Organization
                  name:University of Tehran
                  address:
                     name:Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Ajay Goel
            affiliation:
                  name:Baylor University Medical Center
                  address:
                     name:Gastrointestinal Cancer Research Laboratory, Division of Gastroenterology, Baylor Research Institute and Charles A. Sammons Cancer Center, Baylor University Medical Center, Dallas, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Constanze Buhrmann
            affiliation:
                  name:Ludwig-Maximilian-University Munich
                  address:
                     name:Institute of Anatomy, Ludwig-Maximilian-University Munich, Munich, Germany
                     type:PostalAddress
                  type:Organization
            type:Person
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      address:
         name:Department of Anatomy and Cell Biology, Ludwig-Maximilian-University Munich, Munich, Germany
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      address:
         name:Investigating Institute of Molecular Biological System Transfer, Tehran, Iran
         type:PostalAddress
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         name:Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
         type:PostalAddress
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      address:
         name:Gastrointestinal Cancer Research Laboratory, Division of Gastroenterology, Baylor Research Institute and Charles A. Sammons Cancer Center, Baylor University Medical Center, Dallas, USA
         type:PostalAddress
      name:Ludwig-Maximilian-University Munich
      address:
         name:Institute of Anatomy, Ludwig-Maximilian-University Munich, Munich, Germany
         type:PostalAddress
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Person:
      name:Mehdi Shakibaei
      affiliation:
            name:Ludwig-Maximilian-University Munich
            address:
               name:Institute of Anatomy, Ludwig-Maximilian-University Munich, Munich, Germany
               type:PostalAddress
            type:Organization
      email:[email protected]
      name:Patricia Kraehe
      affiliation:
            name:Ludwig-Maximilian-University Munich
            address:
               name:Institute of Anatomy, Ludwig-Maximilian-University Munich, Munich, Germany
               type:PostalAddress
            type:Organization
      name:Bastian Popper
      affiliation:
            name:Ludwig-Maximilian-University Munich
            address:
               name:Department of Anatomy and Cell Biology, Ludwig-Maximilian-University Munich, Munich, Germany
               type:PostalAddress
            type:Organization
      name:Parviz Shayan
      affiliation:
            name:Investigating Institute of Molecular Biological System Transfer
            address:
               name:Investigating Institute of Molecular Biological System Transfer, Tehran, Iran
               type:PostalAddress
            type:Organization
            name:University of Tehran
            address:
               name:Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
               type:PostalAddress
            type:Organization
      name:Ajay Goel
      affiliation:
            name:Baylor University Medical Center
            address:
               name:Gastrointestinal Cancer Research Laboratory, Division of Gastroenterology, Baylor Research Institute and Charles A. Sammons Cancer Center, Baylor University Medical Center, Dallas, USA
               type:PostalAddress
            type:Organization
      name:Constanze Buhrmann
      affiliation:
            name:Ludwig-Maximilian-University Munich
            address:
               name:Institute of Anatomy, Ludwig-Maximilian-University Munich, Munich, Germany
               type:PostalAddress
            type:Organization
PostalAddress:
      name:Institute of Anatomy, Ludwig-Maximilian-University Munich, Munich, Germany
      name:Institute of Anatomy, Ludwig-Maximilian-University Munich, Munich, Germany
      name:Department of Anatomy and Cell Biology, Ludwig-Maximilian-University Munich, Munich, Germany
      name:Investigating Institute of Molecular Biological System Transfer, Tehran, Iran
      name:Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
      name:Gastrointestinal Cancer Research Laboratory, Division of Gastroenterology, Baylor Research Institute and Charles A. Sammons Cancer Center, Baylor University Medical Center, Dallas, USA
      name:Institute of Anatomy, Ludwig-Maximilian-University Munich, Munich, Germany

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