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Keywords {🔍}

cancer, pubmed, resistance, cells, drug, article, google, scholar, cas, crisprcas, study, central, cell, showed, gene, technology, cancers, involved, treatment, results, dna, studies, targeting, expression, system, genes, repair, signaling, drugs, role, technique, important, wang, editing, zhang, chemotherapy, breast, target, effects, mechanisms, sensitivity, inhibition, shown, enhance, doxorubicin, genomewide, liu, overcoming, overcome, lung,

Topics {✒️}

gag//gtaa ac → gag//gtaa gt genome-wide crispr/cas9 screens genome-wide crispr/cas9 screen crispr/cas9-mediated genome engineering crispr/cas9-based genome editing crispr/cas9 gene-editing technology crispr/cas9-mediated gene knockout crispr/cas9 genome-wide loss crispr/cas9 gene-editing technique crisp/cas9 gene-editing technique cas9 generates double-strand break crispr/cas9 genome editing akt-yb1-mdr1 signaling pathway crispr/cas9 gene editing wnt/b-catenin signaling pathway crispr-cas9 knockout screening genome-wide crispr libraries crispr/cas9-mediated knockout p-glycoprotein-mediated multidrug resistance p-gp-overexpressing mcf-7 cells article vaghari-tabari etoposide-resistant leukemia cells mammalian n6-methyladenosine sites kras/braf-mutated colorectal cancers writing—original draft preparation triple-negative breast cancer crispr/cas9-mediated mutagenesis dna-targeting anti-cancer therapies drug-resistant cell lines crispr-cas9-mediated silencing article download pdf muscle-invasive bladder cancer pi3k-akt-mtor pathway pi3k/akt/mtor pathway small-cell lung cancer crispr/cas9-mediated inhibition cutaneous t-cell lymphoma crispr/cas9 technique enhances cas9-mediated gene disruption multidrug-resistant cancer cells genome editing techniques target single-strand break drug-resistant cancer cell create single-strand breaks cisplatin-mediated cell death suppressing mdr1/p-gp expression drug-resistant colorectal cancer central nervous system platelet-derived growth factor crispr/cas9 system delivery

Questions {❓}

• Limitation of CRISPR/Cas9 gene editing: is there a long way to the clinic?
• The role of DNA damage response in chemo- and radio-resistance of cancer cells: can DDR inhibitors sole the problem?
• Tyrosine kinase inhibitors in chronic myeloid leukaemia: which, when, for whom?

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         description:The CRISPR/Cas9 system is an RNA-based adaptive immune system in bacteria and archaea. Various studies have shown that it is possible to target a wide range of human genes and treat some human diseases, including cancers, by the CRISPR/Cas9 system. In fact, CRISPR/Cas9 gene editing is one of the most efficient genome manipulation techniques. Studies have shown that CRISPR/Cas9 technology, in addition to having the potential to be used as a new therapeutic approach in the treatment of cancers, can also be used to enhance the effectiveness of existing treatments. Undoubtedly, the issue of drug resistance is one of the main obstacles in the treatment of cancers. Cancer cells resist anticancer drugs by a variety of mechanisms, such as enhancing anticancer drugs efflux, enhancing DNA repair, enhancing stemness, and attenuating apoptosis. Mutations in some proteins of different cellular signaling pathways are associated with these events and drug resistance. Recent studies have shown that the CRISPR/Cas9 technique can be used to target important genes involved in these mechanisms, thereby increasing the effectiveness of anticancer drugs. In this review article, studies related to the applications of this technique in overcoming drug resistance in cancer cells will be reviewed. In addition, we will give a brief overview of the limitations of the CRISP/Cas9 gene-editing technique.
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