We are analyzing https://link.springer.com/article/10.1186/jbiol10.

Title:
The phosphatidylserine receptor has essential functions during embryogenesis but not in apoptotic cell removal | Journal of Biology
Description:
Background Phagocytosis of apoptotic cells is fundamental to animal development, immune function and cellular homeostasis. The phosphatidylserine receptor (Ptdsr) on phagocytes has been implicated in the recognition and engulfment of apoptotic cells and in anti-inflammatory signaling. To determine the biological function of the phosphatidylserine receptor in vivo, we inactivated the Ptdsr gene in the mouse. Results Ablation of Ptdsr function in mice causes perinatal lethality, growth retardation and a delay in terminal differentiation of the kidney, intestine, liver and lungs during embryogenesis. Moreover, eye development can be severely disturbed, ranging from defects in retinal differentiation to complete unilateral or bilateral absence of eyes. Ptdsr -/- mice with anophthalmia develop novel lesions, with induction of ectopic retinal-pigmented epithelium in nasal cavities. A comprehensive investigation of apoptotic cell clearance in vivo and in vitro demonstrated that engulfment of apoptotic cells was normal in Ptdsr knockout mice, but Ptdsr-deficient macrophages were impaired in pro- and anti-inflammatory cytokine signaling after stimulation with apoptotic cells or with lipopolysaccharide. Conclusion Ptdsr is essential for the development and differentiation of multiple organs during embryogenesis but not for apoptotic cell removal. Ptdsr may thus have a novel, unexpected developmental function as an important differentiation-promoting gene. Moreover, Ptdsr is not required for apoptotic cell clearance by macrophages but seems to be necessary for the regulation of macrophage cytokine responses. These results clearly contradict the current view that the phosphatidylserine receptor primarily functions in apoptotic cell clearance.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Science
Education
Telecommunications

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 7,626,432 visitors per month in the current month.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We find it hard to spot revenue streams.

The purpose of some websites isn't monetary gain; they're meant to inform, educate, or foster collaboration. Everyone has unique reasons for building websites. This could be an example. Link.springer.com has a revenue plan, but it's either invisible or we haven't found it.

Keywords {🔍}

ptdsr, cells, apoptotic, mice, cell, embryos, figure, macrophages, pubmed, article, wildtype, development, google, scholar, cas, analysis, clearance, differentiation, phagocytosis, receptor, ptdsrdeficient, expression, phosphatidylserine, observed, apoptosis, number, data, normal, ptdsrknockout, lung, engulfment, retinal, tissue, death, mutant, embryogenesis, mouse, eye, developing, recognition, gene, results, sections, found, homozygous, tissues, differences, lungs, defects, macrophage,

Topics {✒️}

bacterial artificial chromosome β-geo gene-trap vector mount β-galactosidase staining isogenic c57bl/6j background wilcoxon-signed rank test oncogene-expressing mammary acini es-cell clones resistant intensive β-galactosidase staining mutant gene-trap allele crkii/dock180/rac pathway tumor necrosis factor-α es-cell line carrying pure c57bl/6j background kpni-linearized targeting vector obtain ptdsr-deficient progeny article download pdf neomycin-selection cassette full-length ptdsr cdna release immune-suppressive cytokines phosphatidylserine-dependent β2-glycoprotein confirm germ-line transmission sds-polyacrylamide gel electrophoresis specific pathogen-free environment single es-cell line selected es-cell clones ptdsr gene-targeting strategy anti-inflammatory cytokine signaling ectopic retinal-pigmented epithelium ptdsr-deficient mouse lines mfg-e8-deficient mice macrophageless sfpi1-knockout embryos bmc cell biol lps-induced tnf-α release central mantle layer x-gal-stained embryos thioglycollate-elicited peritoneal macrophages mutant embryo release anti-inflammatory cytokines cell-corpse engulfment pathways coding exons i-vi germ-line chimeras tunel-positive cell corpses ptdsr-deficient homozygous embryos authors’ original file unphagocytosed tunel-positive cells ptdsr-deficient homozygotes gave β-galactosidase staining fetal-liver-derived macrophages fetal liver-derived macrophages ptdsr-knockout mice die

Questions {❓}

Henson PM, Bratton DL, Fadok VA: The phosphatidylserine receptor: a crucial molecular switch?

Schema {🗺️}

WebPage:
      mainEntity:
         headline:The phosphatidylserine receptor has essential functions during embryogenesis but not in apoptotic cell removal
         description:Phagocytosis of apoptotic cells is fundamental to animal development, immune function and cellular homeostasis. The phosphatidylserine receptor (Ptdsr) on phagocytes has been implicated in the recognition and engulfment of apoptotic cells and in anti-inflammatory signaling. To determine the biological function of the phosphatidylserine receptor in vivo, we inactivated the Ptdsr gene in the mouse. Ablation of Ptdsr function in mice causes perinatal lethality, growth retardation and a delay in terminal differentiation of the kidney, intestine, liver and lungs during embryogenesis. Moreover, eye development can be severely disturbed, ranging from defects in retinal differentiation to complete unilateral or bilateral absence of eyes. Ptdsr -/- mice with anophthalmia develop novel lesions, with induction of ectopic retinal-pigmented epithelium in nasal cavities. A comprehensive investigation of apoptotic cell clearance in vivo and in vitro demonstrated that engulfment of apoptotic cells was normal in Ptdsr knockout mice, but Ptdsr-deficient macrophages were impaired in pro- and anti-inflammatory cytokine signaling after stimulation with apoptotic cells or with lipopolysaccharide. Ptdsr is essential for the development and differentiation of multiple organs during embryogenesis but not for apoptotic cell removal. Ptdsr may thus have a novel, unexpected developmental function as an important differentiation-promoting gene. Moreover, Ptdsr is not required for apoptotic cell clearance by macrophages but seems to be necessary for the regulation of macrophage cytokine responses. These results clearly contradict the current view that the phosphatidylserine receptor primarily functions in apoptotic cell clearance.
         datePublished:2004-08-23T00:00:00Z
         dateModified:2004-08-23T00:00:00Z
         pageStart:1
         pageEnd:18
         license:http://creativecommons.org/licenses/by/2.0/
         sameAs:https://doi.org/10.1186/jbiol10
         keywords:
            Apoptotic Cell
            Bacterial Artificial Chromosome
            Additional Data File
            Apoptotic Cell Clearance
            Homozygous Embryo
            Life Sciences
            general
            Biomedicine
         image:
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fjbiol10/MediaObjects/13061_2004_Article_47_Fig1_HTML.jpg
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fjbiol10/MediaObjects/13061_2004_Article_47_Fig2_HTML.jpg
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fjbiol10/MediaObjects/13061_2004_Article_47_Fig3_HTML.jpg
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fjbiol10/MediaObjects/13061_2004_Article_47_Fig4_HTML.jpg
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fjbiol10/MediaObjects/13061_2004_Article_47_Fig5_HTML.jpg
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fjbiol10/MediaObjects/13061_2004_Article_47_Fig6_HTML.jpg
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fjbiol10/MediaObjects/13061_2004_Article_47_Fig7_HTML.jpg
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fjbiol10/MediaObjects/13061_2004_Article_47_Fig8_HTML.jpg
         isPartOf:
            name:Journal of Biology
            issn:
               1475-4924
            volumeNumber:3
            type:
               Periodical
               PublicationVolume
         publisher:
            name:BioMed Central
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Jens Böse
               affiliation:
                     name:German Research Center for Biotechnology (GBF)
                     address:
                        name:Junior Research Group Infection Genetics, German Research Center for Biotechnology (GBF), Braunschweig, Germany
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Achim D Gruber
               affiliation:
                     name:School of Veterinary Medicine Hannover
                     address:
                        name:Department of Pathology, School of Veterinary Medicine Hannover, Hannover, Germany
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Laura Helming
               affiliation:
                     name:German Research Center for Biotechnology (GBF)
                     address:
                        name:Junior Research Group Infection Genetics, German Research Center for Biotechnology (GBF), Braunschweig, Germany
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Stefanie Schiebe
               affiliation:
                     name:German Research Center for Biotechnology (GBF)
                     address:
                        name:Junior Research Group Infection Genetics, German Research Center for Biotechnology (GBF), Braunschweig, Germany
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Ivonne Wegener
               affiliation:
                     name:German Research Center for Biotechnology (GBF)
                     address:
                        name:Junior Research Group Infection Genetics, German Research Center for Biotechnology (GBF), Braunschweig, Germany
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Martin Hafner
               affiliation:
                     name:German Research Center for Biotechnology (GBF)
                     address:
                        name:Department of Experimental Immunology, German Research Center for Biotechnology (GBF), Braunschweig, Germany
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Marianne Beales
               affiliation:
                     name:Ozgene Pty. Ltd.
                     address:
                        name:Ozgene Pty. Ltd., Canning Vale, Australia
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Frank Köntgen
               affiliation:
                     name:Ozgene Pty. Ltd.
                     address:
                        name:Ozgene Pty. Ltd., Canning Vale, Australia
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Andreas Lengeling
               affiliation:
                     name:German Research Center for Biotechnology (GBF)
                     address:
                        name:Junior Research Group Infection Genetics, German Research Center for Biotechnology (GBF), Braunschweig, Germany
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
         isAccessibleForFree:1
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:The phosphatidylserine receptor has essential functions during embryogenesis but not in apoptotic cell removal
      description:Phagocytosis of apoptotic cells is fundamental to animal development, immune function and cellular homeostasis. The phosphatidylserine receptor (Ptdsr) on phagocytes has been implicated in the recognition and engulfment of apoptotic cells and in anti-inflammatory signaling. To determine the biological function of the phosphatidylserine receptor in vivo, we inactivated the Ptdsr gene in the mouse. Ablation of Ptdsr function in mice causes perinatal lethality, growth retardation and a delay in terminal differentiation of the kidney, intestine, liver and lungs during embryogenesis. Moreover, eye development can be severely disturbed, ranging from defects in retinal differentiation to complete unilateral or bilateral absence of eyes. Ptdsr -/- mice with anophthalmia develop novel lesions, with induction of ectopic retinal-pigmented epithelium in nasal cavities. A comprehensive investigation of apoptotic cell clearance in vivo and in vitro demonstrated that engulfment of apoptotic cells was normal in Ptdsr knockout mice, but Ptdsr-deficient macrophages were impaired in pro- and anti-inflammatory cytokine signaling after stimulation with apoptotic cells or with lipopolysaccharide. Ptdsr is essential for the development and differentiation of multiple organs during embryogenesis but not for apoptotic cell removal. Ptdsr may thus have a novel, unexpected developmental function as an important differentiation-promoting gene. Moreover, Ptdsr is not required for apoptotic cell clearance by macrophages but seems to be necessary for the regulation of macrophage cytokine responses. These results clearly contradict the current view that the phosphatidylserine receptor primarily functions in apoptotic cell clearance.
      datePublished:2004-08-23T00:00:00Z
      dateModified:2004-08-23T00:00:00Z
      pageStart:1
      pageEnd:18
      license:http://creativecommons.org/licenses/by/2.0/
      sameAs:https://doi.org/10.1186/jbiol10
      keywords:
         Apoptotic Cell
         Bacterial Artificial Chromosome
         Additional Data File
         Apoptotic Cell Clearance
         Homozygous Embryo
         Life Sciences
         general
         Biomedicine
      image:
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fjbiol10/MediaObjects/13061_2004_Article_47_Fig1_HTML.jpg
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fjbiol10/MediaObjects/13061_2004_Article_47_Fig2_HTML.jpg
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fjbiol10/MediaObjects/13061_2004_Article_47_Fig3_HTML.jpg
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fjbiol10/MediaObjects/13061_2004_Article_47_Fig4_HTML.jpg
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fjbiol10/MediaObjects/13061_2004_Article_47_Fig5_HTML.jpg
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fjbiol10/MediaObjects/13061_2004_Article_47_Fig6_HTML.jpg
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fjbiol10/MediaObjects/13061_2004_Article_47_Fig7_HTML.jpg
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fjbiol10/MediaObjects/13061_2004_Article_47_Fig8_HTML.jpg
      isPartOf:
         name:Journal of Biology
         issn:
            1475-4924
         volumeNumber:3
         type:
            Periodical
            PublicationVolume
      publisher:
         name:BioMed Central
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Jens Böse
            affiliation:
                  name:German Research Center for Biotechnology (GBF)
                  address:
                     name:Junior Research Group Infection Genetics, German Research Center for Biotechnology (GBF), Braunschweig, Germany
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Achim D Gruber
            affiliation:
                  name:School of Veterinary Medicine Hannover
                  address:
                     name:Department of Pathology, School of Veterinary Medicine Hannover, Hannover, Germany
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Laura Helming
            affiliation:
                  name:German Research Center for Biotechnology (GBF)
                  address:
                     name:Junior Research Group Infection Genetics, German Research Center for Biotechnology (GBF), Braunschweig, Germany
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Stefanie Schiebe
            affiliation:
                  name:German Research Center for Biotechnology (GBF)
                  address:
                     name:Junior Research Group Infection Genetics, German Research Center for Biotechnology (GBF), Braunschweig, Germany
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Ivonne Wegener
            affiliation:
                  name:German Research Center for Biotechnology (GBF)
                  address:
                     name:Junior Research Group Infection Genetics, German Research Center for Biotechnology (GBF), Braunschweig, Germany
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Martin Hafner
            affiliation:
                  name:German Research Center for Biotechnology (GBF)
                  address:
                     name:Department of Experimental Immunology, German Research Center for Biotechnology (GBF), Braunschweig, Germany
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Marianne Beales
            affiliation:
                  name:Ozgene Pty. Ltd.
                  address:
                     name:Ozgene Pty. Ltd., Canning Vale, Australia
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Frank Köntgen
            affiliation:
                  name:Ozgene Pty. Ltd.
                  address:
                     name:Ozgene Pty. Ltd., Canning Vale, Australia
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Andreas Lengeling
            affiliation:
                  name:German Research Center for Biotechnology (GBF)
                  address:
                     name:Junior Research Group Infection Genetics, German Research Center for Biotechnology (GBF), Braunschweig, Germany
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      isAccessibleForFree:1
["Periodical","PublicationVolume"]:
      name:Journal of Biology
      issn:
         1475-4924
      volumeNumber:3
Organization:
      name:BioMed Central
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:German Research Center for Biotechnology (GBF)
      address:
         name:Junior Research Group Infection Genetics, German Research Center for Biotechnology (GBF), Braunschweig, Germany
         type:PostalAddress
      name:School of Veterinary Medicine Hannover
      address:
         name:Department of Pathology, School of Veterinary Medicine Hannover, Hannover, Germany
         type:PostalAddress
      name:German Research Center for Biotechnology (GBF)
      address:
         name:Junior Research Group Infection Genetics, German Research Center for Biotechnology (GBF), Braunschweig, Germany
         type:PostalAddress
      name:German Research Center for Biotechnology (GBF)
      address:
         name:Junior Research Group Infection Genetics, German Research Center for Biotechnology (GBF), Braunschweig, Germany
         type:PostalAddress
      name:German Research Center for Biotechnology (GBF)
      address:
         name:Junior Research Group Infection Genetics, German Research Center for Biotechnology (GBF), Braunschweig, Germany
         type:PostalAddress
      name:German Research Center for Biotechnology (GBF)
      address:
         name:Department of Experimental Immunology, German Research Center for Biotechnology (GBF), Braunschweig, Germany
         type:PostalAddress
      name:Ozgene Pty. Ltd.
      address:
         name:Ozgene Pty. Ltd., Canning Vale, Australia
         type:PostalAddress
      name:Ozgene Pty. Ltd.
      address:
         name:Ozgene Pty. Ltd., Canning Vale, Australia
         type:PostalAddress
      name:German Research Center for Biotechnology (GBF)
      address:
         name:Junior Research Group Infection Genetics, German Research Center for Biotechnology (GBF), Braunschweig, Germany
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Jens Böse
      affiliation:
            name:German Research Center for Biotechnology (GBF)
            address:
               name:Junior Research Group Infection Genetics, German Research Center for Biotechnology (GBF), Braunschweig, Germany
               type:PostalAddress
            type:Organization
      name:Achim D Gruber
      affiliation:
            name:School of Veterinary Medicine Hannover
            address:
               name:Department of Pathology, School of Veterinary Medicine Hannover, Hannover, Germany
               type:PostalAddress
            type:Organization
      name:Laura Helming
      affiliation:
            name:German Research Center for Biotechnology (GBF)
            address:
               name:Junior Research Group Infection Genetics, German Research Center for Biotechnology (GBF), Braunschweig, Germany
               type:PostalAddress
            type:Organization
      name:Stefanie Schiebe
      affiliation:
            name:German Research Center for Biotechnology (GBF)
            address:
               name:Junior Research Group Infection Genetics, German Research Center for Biotechnology (GBF), Braunschweig, Germany
               type:PostalAddress
            type:Organization
      name:Ivonne Wegener
      affiliation:
            name:German Research Center for Biotechnology (GBF)
            address:
               name:Junior Research Group Infection Genetics, German Research Center for Biotechnology (GBF), Braunschweig, Germany
               type:PostalAddress
            type:Organization
      name:Martin Hafner
      affiliation:
            name:German Research Center for Biotechnology (GBF)
            address:
               name:Department of Experimental Immunology, German Research Center for Biotechnology (GBF), Braunschweig, Germany
               type:PostalAddress
            type:Organization
      name:Marianne Beales
      affiliation:
            name:Ozgene Pty. Ltd.
            address:
               name:Ozgene Pty. Ltd., Canning Vale, Australia
               type:PostalAddress
            type:Organization
      name:Frank Köntgen
      affiliation:
            name:Ozgene Pty. Ltd.
            address:
               name:Ozgene Pty. Ltd., Canning Vale, Australia
               type:PostalAddress
            type:Organization
      name:Andreas Lengeling
      affiliation:
            name:German Research Center for Biotechnology (GBF)
            address:
               name:Junior Research Group Infection Genetics, German Research Center for Biotechnology (GBF), Braunschweig, Germany
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Junior Research Group Infection Genetics, German Research Center for Biotechnology (GBF), Braunschweig, Germany
      name:Department of Pathology, School of Veterinary Medicine Hannover, Hannover, Germany
      name:Junior Research Group Infection Genetics, German Research Center for Biotechnology (GBF), Braunschweig, Germany
      name:Junior Research Group Infection Genetics, German Research Center for Biotechnology (GBF), Braunschweig, Germany
      name:Junior Research Group Infection Genetics, German Research Center for Biotechnology (GBF), Braunschweig, Germany
      name:Department of Experimental Immunology, German Research Center for Biotechnology (GBF), Braunschweig, Germany
      name:Ozgene Pty. Ltd., Canning Vale, Australia
      name:Ozgene Pty. Ltd., Canning Vale, Australia
      name:Junior Research Group Infection Genetics, German Research Center for Biotechnology (GBF), Braunschweig, Germany

External Links {🔗}(208)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

CDN Services {📦}

Crossref

5.1s.

LINK . SPRINGER . COM {}