Here's how LINK.SPRINGER.COM makes money* and how much!

*Please read our disclaimer before using our estimates.
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LINK . SPRINGER . COM {}

  1. Analyzed Page
  2. Matching Content Categories
  3. CMS
  4. Monthly Traffic Estimate
  5. How Does Link.springer.com Make Money
  6. Keywords
  7. Topics
  8. Questions
  9. Schema
  10. External Links
  11. Analytics And Tracking
  12. Libraries
  13. CDN Services

We are analyzing https://link.springer.com/article/10.1186/1750-1326-7-8.

Title:
Intraneuronal Aβ detection in 5xFAD mice by a new Aβ-specific antibody | Molecular Neurodegeneration
Description:
Background The form(s) of amyloid-β peptide (Aβ) associated with the pathology characteristic of Alzheimer
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

  • Education
  • Science
  • Telecommunications

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month


Based on our best estimate, this website will receive around 7,642,828 visitors per month in the current month.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

The income method remains a mystery to us.

Not all websites are made for profit; some exist to inform or educate users. Or any other reason why people make websites. And this might be the case. Link.springer.com has a revenue plan, but it's either invisible or we haven't found it.

Keywords {🔍}

moab, intraneuronal, mice, pubmed, article, app, google, scholar, cas, xfad, antibody, antibodies, figure, alzheimers, brain, xtg, mouse, disease, amyloid, abeta, detection, accumulation, tissue, extracellular, analysis, human, min, immunoreactivity, sections, transgenic, ihc, cell, plaque, staining, month, central, deposition, protein, peptide, pathology, mgml, detected, pmol, incubated, cterminal, cortex, results, residues, demonstrated, authors,

Topics {✒️}

amino-truncated amyloid beta-peptide open access article rabbit-anti-mouse antibody conjugated article download pdf impaired long-term potentiation hrp-conjugated secondary antibodies mary jo ladu amyloid-beta42 alters apolipoprotein aβ-tg mouse models nih/nia t32-ag06697 beta-amyloid precursor protein blaine stine jr & arlene antibody-binding saturation curve tg-arcswe mice treated tg-arcswe/bace1-/- mice app c-terminal fragments biotinylated anti-igg antibody dimer/trimers induce neurotoxicity c-terminal antibodies specific c-terminal specific antibodies pan-specific monoclonal antibody hek-appswe/bace1 cells time resolved eu-fluorescence antibody-dependent saturation curve disease-related cognitive deficits intraneuronal beta-amyloid accumulation ad-susceptible brain regions {beta}-mediated alzheimer neurodegeneration intraneuronal beta-amyloid aggregates presenilin-1 double-transgenic mice amyloid beta-peptide levels lithium dodecyl sulphate aβ-tg mouse model pan-specific aβ antibody cell culture media/lysates app c-terminal antibody provided hek-appswe/bace1 cognitive impairment pre-mortem amyloid-beta peptide oligomerization targeting amyloid-beta peptide anti-app c-terminal gouras gk c-terminal app antibodies blaine stine jr c-terminal fragments sara-claude michon biotinylated secondary antibody anti-app n-terminal global pharmaceutical research privacy choices/manage cookies

Questions {❓}

  • Golde TE, Janus C: Homing in on intracellular Abeta?

Schema {🗺️}

WebPage:
      mainEntity:
         headline:Intraneuronal Aβ detection in 5xFAD mice by a new Aβ-specific antibody
         description:The form(s) of amyloid-β peptide (Aβ) associated with the pathology characteristic of Alzheimer's disease (AD) remains unclear. In particular, the neurotoxicity of intraneuronal Aβ accumulation is an issue of considerable controversy; even the existence of Aβ deposits within neurons has recently been challenged by Winton and co-workers. These authors purport that it is actually intraneuronal APP that is being detected by antibodies thought to be specific for Aβ. To further address this issue, an anti-Aβ antibody was developed (MOAB-2) that specifically detects Aβ, but not APP. This antibody allows for the further evaluation of the early accumulation of intraneuronal Aβ in transgenic mice with increased levels of human Aβ in 5xFAD and 3xTg mice. MOAB-2 (mouse IgG2b) is a pan-specific, high-titer antibody to Aβ residues 1-4 as demonstrated by biochemical and immunohistochemical analyses (IHC), particularly compared to 6E10 (a commonly used commercial antibody to Aβ residues 3-8). MOAB-2 did not detect APP or APP-CTFs in cell culture media/lysates (HEK-APPSwe or HEK-APPSwe/BACE1) or in brain homogenates from transgenic mice expressing 5 familial AD (FAD) mutation (5xFAD mice). Using IHC on 5xFAD brain tissue, MOAB-2 immunoreactivity co-localized with C-terminal antibodies specific for Aβ40 and Aβ42. MOAB-2 did not co-localize with either N- or C-terminal antibodies to APP. In addition, no MOAB-2-immunreactivity was observed in the brains of 5xFAD/BACE-/- mice, although significant amounts of APP were detected by N- and C-terminal antibodies to APP, as well as by 6E10. In both 5xFAD and 3xTg mouse brain tissue, MOAB-2 co-localized with cathepsin-D, a marker for acidic organelles, further evidence for intraneuronal Aβ, distinct from Aβ associated with the cell membrane. MOAB-2 demonstrated strong intraneuronal and extra-cellular immunoreactivity in 5xFAD and 3xTg mouse brain tissues. Both intraneuronal Aβ accumulation and extracellular Aβ deposition was demonstrated in 5xFAD mice and 3xTg mice with MOAB-2, an antibody that will help differentiate intracellular Aβ from APP. However, further investigation is required to determine whether a molecular mechanism links the presence of intraneuronal Aβ with neurotoxicity. As well, understanding the relevance of these observations to human AD patients is critical.
         datePublished:2012-03-16T00:00:00Z
         dateModified:2012-03-16T00:00:00Z
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            APP
            MOAB-2
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            5xFAD
            Antibody
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            Neurosciences
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            Molecular Medicine
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                        name:Department of Anatomy and Cell Biology, University of Illinois at Chicago, Chicago, USA
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      headline:Intraneuronal Aβ detection in 5xFAD mice by a new Aβ-specific antibody
      description:The form(s) of amyloid-β peptide (Aβ) associated with the pathology characteristic of Alzheimer's disease (AD) remains unclear. In particular, the neurotoxicity of intraneuronal Aβ accumulation is an issue of considerable controversy; even the existence of Aβ deposits within neurons has recently been challenged by Winton and co-workers. These authors purport that it is actually intraneuronal APP that is being detected by antibodies thought to be specific for Aβ. To further address this issue, an anti-Aβ antibody was developed (MOAB-2) that specifically detects Aβ, but not APP. This antibody allows for the further evaluation of the early accumulation of intraneuronal Aβ in transgenic mice with increased levels of human Aβ in 5xFAD and 3xTg mice. MOAB-2 (mouse IgG2b) is a pan-specific, high-titer antibody to Aβ residues 1-4 as demonstrated by biochemical and immunohistochemical analyses (IHC), particularly compared to 6E10 (a commonly used commercial antibody to Aβ residues 3-8). MOAB-2 did not detect APP or APP-CTFs in cell culture media/lysates (HEK-APPSwe or HEK-APPSwe/BACE1) or in brain homogenates from transgenic mice expressing 5 familial AD (FAD) mutation (5xFAD mice). Using IHC on 5xFAD brain tissue, MOAB-2 immunoreactivity co-localized with C-terminal antibodies specific for Aβ40 and Aβ42. MOAB-2 did not co-localize with either N- or C-terminal antibodies to APP. In addition, no MOAB-2-immunreactivity was observed in the brains of 5xFAD/BACE-/- mice, although significant amounts of APP were detected by N- and C-terminal antibodies to APP, as well as by 6E10. In both 5xFAD and 3xTg mouse brain tissue, MOAB-2 co-localized with cathepsin-D, a marker for acidic organelles, further evidence for intraneuronal Aβ, distinct from Aβ associated with the cell membrane. MOAB-2 demonstrated strong intraneuronal and extra-cellular immunoreactivity in 5xFAD and 3xTg mouse brain tissues. Both intraneuronal Aβ accumulation and extracellular Aβ deposition was demonstrated in 5xFAD mice and 3xTg mice with MOAB-2, an antibody that will help differentiate intracellular Aβ from APP. However, further investigation is required to determine whether a molecular mechanism links the presence of intraneuronal Aβ with neurotoxicity. As well, understanding the relevance of these observations to human AD patients is critical.
      datePublished:2012-03-16T00:00:00Z
      dateModified:2012-03-16T00:00:00Z
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      license:https://creativecommons.org/licenses/by/2.0
      sameAs:https://doi.org/10.1186/1750-1326-7-8
      keywords:
         Intraneuronal
         Aβ
         APP
         MOAB-2
         3xTg
         5xFAD
         Antibody
         Alzheimer's disease
         Neurosciences
         Neurology
         Molecular Medicine
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         name:BioMed Central
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            type:ImageObject
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            type:Person
            name:W Blaine Stine Jr
            affiliation:
                  name:Evanston Northwestern Healthcare Research Institute
                  address:
                     name:Department of Medicine, Division of Geriatrics, Evanston Northwestern Healthcare Research Institute, Evanston Illinois, USA
                     type:PostalAddress
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                  name:Abbott Bioresearch Center
                  address:
                     name:CMC Program Management, Global Pharmaceutical Operations, Abbott Bioresearch Center, Worcester, USA
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            name:Sara-Claude Michon
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                  address:
                     name:Department of Neurological Sciences, Rush University Medical Center, Chicago, USA
                     type:PostalAddress
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            type:Person
            name:Evelyn Nwabuisi-Heath
            affiliation:
                  name:University of Illinois at Chicago
                  address:
                     name:Department of Anatomy and Cell Biology, University of Illinois at Chicago, Chicago, USA
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                  address:
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            name:Chunjiang Yu
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                  name:University of Illinois at Chicago
                  address:
                     name:Department of Anatomy and Cell Biology, University of Illinois at Chicago, Chicago, USA
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            name:Dean M Hartley
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                  name:Rush University Medical Center
                  address:
                     name:Department of Neurological Sciences, Rush University Medical Center, Chicago, USA
                     type:PostalAddress
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            name:Mary Jo LaDu
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                  name:University of Illinois at Chicago
                  address:
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      name:Leon M Tai
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            name:University of Illinois at Chicago
            address:
               name:Department of Anatomy and Cell Biology, University of Illinois at Chicago, Chicago, USA
               type:PostalAddress
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      name:Takahisa Kanekiyo
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            name:Mayo Clinic
            address:
               name:Department of Neuroscience, Mayo Clinic, Jacksonville, USA
               type:PostalAddress
            type:Organization
      name:W Blaine Stine Jr
      affiliation:
            name:Evanston Northwestern Healthcare Research Institute
            address:
               name:Department of Medicine, Division of Geriatrics, Evanston Northwestern Healthcare Research Institute, Evanston Illinois, USA
               type:PostalAddress
            type:Organization
            name:Abbott Bioresearch Center
            address:
               name:CMC Program Management, Global Pharmaceutical Operations, Abbott Bioresearch Center, Worcester, USA
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      name:Sara-Claude Michon
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            name:Rush University Medical Center
            address:
               name:Department of Neurological Sciences, Rush University Medical Center, Chicago, USA
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      name:Evelyn Nwabuisi-Heath
      affiliation:
            name:University of Illinois at Chicago
            address:
               name:Department of Anatomy and Cell Biology, University of Illinois at Chicago, Chicago, USA
               type:PostalAddress
            type:Organization
      name:Arlene M Manelli
      affiliation:
            name:Evanston Northwestern Healthcare Research Institute
            address:
               name:Department of Medicine, Division of Geriatrics, Evanston Northwestern Healthcare Research Institute, Evanston Illinois, USA
               type:PostalAddress
            type:Organization
            name:Neuroscience Research, Global Pharmaceutical Research and Development, Abbott
            address:
               name:Neuroscience Research, Global Pharmaceutical Research and Development, Abbott, Abbott Park, USA
               type:PostalAddress
            type:Organization
      name:Yifan Fu
      affiliation:
            name:Northwestern University
            address:
               name:Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Chicago, USA
               type:PostalAddress
            type:Organization
      name:Sean Riordan
      affiliation:
            name:Northwestern University
            address:
               name:Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Chicago, USA
               type:PostalAddress
            type:Organization
      name:William A Eimer
      affiliation:
            name:Northwestern University
            address:
               name:Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Chicago, USA
               type:PostalAddress
            type:Organization
      name:Lester Binder
      affiliation:
            name:Northwestern University
            address:
               name:Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Chicago, USA
               type:PostalAddress
            type:Organization
      name:Guojun Bu
      affiliation:
            name:Mayo Clinic
            address:
               name:Department of Neuroscience, Mayo Clinic, Jacksonville, USA
               type:PostalAddress
            type:Organization
      name:Chunjiang Yu
      affiliation:
            name:University of Illinois at Chicago
            address:
               name:Department of Anatomy and Cell Biology, University of Illinois at Chicago, Chicago, USA
               type:PostalAddress
            type:Organization
      name:Dean M Hartley
      affiliation:
            name:Rush University Medical Center
            address:
               name:Department of Neurological Sciences, Rush University Medical Center, Chicago, USA
               type:PostalAddress
            type:Organization
      name:Mary Jo LaDu
      affiliation:
            name:University of Illinois at Chicago
            address:
               name:Department of Anatomy and Cell Biology, University of Illinois at Chicago, Chicago, USA
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Department of Anatomy and Cell Biology, University of Illinois at Chicago, Chicago, USA
      name:Department of Anatomy and Cell Biology, University of Illinois at Chicago, Chicago, USA
      name:Department of Neuroscience, Mayo Clinic, Jacksonville, USA
      name:Department of Medicine, Division of Geriatrics, Evanston Northwestern Healthcare Research Institute, Evanston Illinois, USA
      name:CMC Program Management, Global Pharmaceutical Operations, Abbott Bioresearch Center, Worcester, USA
      name:Department of Neurological Sciences, Rush University Medical Center, Chicago, USA
      name:Department of Anatomy and Cell Biology, University of Illinois at Chicago, Chicago, USA
      name:Department of Medicine, Division of Geriatrics, Evanston Northwestern Healthcare Research Institute, Evanston Illinois, USA
      name:Neuroscience Research, Global Pharmaceutical Research and Development, Abbott, Abbott Park, USA
      name:Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Chicago, USA
      name:Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Chicago, USA
      name:Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Chicago, USA
      name:Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Chicago, USA
      name:Department of Neuroscience, Mayo Clinic, Jacksonville, USA
      name:Department of Anatomy and Cell Biology, University of Illinois at Chicago, Chicago, USA
      name:Department of Neurological Sciences, Rush University Medical Center, Chicago, USA
      name:Department of Anatomy and Cell Biology, University of Illinois at Chicago, Chicago, USA

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