We are analyzing https://link.springer.com/article/10.1186/1472-6750-4-8.

Title:
Rapid isolation of yeast genomic DNA: Bust n' Grab | BMC Biotechnology
Description:
Background Mutagenesis of yeast artificial chromosomes (YACs) often requires analysis of large numbers of yeast clones to obtain correctly targeted mutants. Conventional ways to isolate yeast genomic DNA utilize either glass beads or enzymatic digestion to disrupt yeast cell wall. Using small glass beads is messy, whereas enzymatic digestion of the cells is expensive when many samples need to be analyzed. We sought to develop an easier and faster protocol than the existing methods for obtaining yeast genomic DNA from liquid cultures or colonies on plates. Results Repeated freeze-thawing of cells in a lysis buffer was used to disrupt the cells and release genomic DNA. Cell lysis was followed by extraction with chloroform and ethanol precipitation of DNA. Two hundred ng – 3 μg of genomic DNA could be isolated from a 1.5 ml overnight liquid culture or from a large colony. Samples were either resuspended directly in a restriction enzyme/RNase coctail mixture for Southern blot hybridization or used for several PCR reactions. We demonstrated the utility of this method by showing an analysis of yeast clones containing a mutagenized human β-globin locus YAC. Conclusion An efficient, inexpensive method for obtaining yeast genomic DNA from liquid cultures or directly from colonies was developed. This protocol circumvents the use of enzymes or glass beads, and therefore is cheaper and easier to perform when processing large numbers of samples.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Science
Education
Telecommunications

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 8,123,328 visitors per month in the current month.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We don't see any clear sign of profit-making.

Many websites are intended to earn money, but some serve to share ideas or build connections. Websites exist for all kinds of purposes. This might be one of them. Link.springer.com might be plotting its profit, but the way they're doing it isn't detectable yet.

Keywords {🔍}

dna, yeast, article, genomic, figure, blot, hybridization, southern, liquid, sequence, google, scholar, pcr, human, yac, site, analysis, large, cell, samples, cultures, method, locus, cas, isolation, artificial, cells, colonies, usa, authors, rapid, access, peterson, glass, beads, protocol, directly, recombination, mutant, fragment, genes, wildtype, information, open, digestion, extraction, ethanol, βglobin, chromosome, mutation,

Topics {✒️}

bust n' grab human β-globin locus southern blot hybridization open access license yeast cell wall simple freeze/thaw cycle ten-minute dna preparation bacteriophage p1 cre-recombinase yeast artificial chromosomes full size image article download pdf yip-mediated mutagenesis results dry ice-ethanol bath wild-type target sequence yip-mediated homologous recombination related subjects mammalian gene regulation conditional gene knockout yeast genomic dna rapid isolation method privacy choices/manage cookies identical loxp sites kansas medical center restriction enzyme digestion yeast integrating plasmid additional asci site full access release genomic dna human globin genes wild-type fragment authors’ original file overnight liquid culture overnight liquid cultures high-frequency transformation liquid cultures giving liquid cultures ranged cell wall mammalian cell culture wild-type sequence repeated freeze-thawing yeast dna isolated simple eukaryotic organism genbank file af137396 shaking water bath hybrid dna molecules rapid isolation γ-globin genes european economic area agarose gel electrophoresis pulled pasteur pipette

Schema {🗺️}

WebPage:
      mainEntity:
         headline:Rapid isolation of yeast genomic DNA: Bust n' Grab
         description:Mutagenesis of yeast artificial chromosomes (YACs) often requires analysis of large numbers of yeast clones to obtain correctly targeted mutants. Conventional ways to isolate yeast genomic DNA utilize either glass beads or enzymatic digestion to disrupt yeast cell wall. Using small glass beads is messy, whereas enzymatic digestion of the cells is expensive when many samples need to be analyzed. We sought to develop an easier and faster protocol than the existing methods for obtaining yeast genomic DNA from liquid cultures or colonies on plates. Repeated freeze-thawing of cells in a lysis buffer was used to disrupt the cells and release genomic DNA. Cell lysis was followed by extraction with chloroform and ethanol precipitation of DNA. Two hundred ng – 3 μg of genomic DNA could be isolated from a 1.5 ml overnight liquid culture or from a large colony. Samples were either resuspended directly in a restriction enzyme/RNase coctail mixture for Southern blot hybridization or used for several PCR reactions. We demonstrated the utility of this method by showing an analysis of yeast clones containing a mutagenized human β-globin locus YAC. An efficient, inexpensive method for obtaining yeast genomic DNA from liquid cultures or directly from colonies was developed. This protocol circumvents the use of enzymes or glass beads, and therefore is cheaper and easier to perform when processing large numbers of samples.
         datePublished:2004-04-21T00:00:00Z
         dateModified:2004-04-21T00:00:00Z
         pageStart:1
         pageEnd:6
         sameAs:https://doi.org/10.1186/1472-6750-4-8
         keywords:
            Globin Gene
            Southern Blot Hybridization
            loxP Site
            Yeast Artificial Chromosome
            Yeast Cell Wall
            Applied Microbiology
            Biotechnology
            Biochemical Engineering
            Genetic Engineering
            Plant Breeding/Biotechnology
            Biomedical Engineering/Biotechnology
         image:
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2F1472-6750-4-8/MediaObjects/12896_2004_Article_65_Fig1_HTML.jpg
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2F1472-6750-4-8/MediaObjects/12896_2004_Article_65_Fig2_HTML.jpg
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2F1472-6750-4-8/MediaObjects/12896_2004_Article_65_Fig3_HTML.jpg
         isPartOf:
            name:BMC Biotechnology
            issn:
               1472-6750
            volumeNumber:4
            type:
               Periodical
               PublicationVolume
         publisher:
            name:BioMed Central
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Susanna Harju
               affiliation:
                     name:University of Kansas Medical Center
                     address:
                        name:Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Halyna Fedosyuk
               affiliation:
                     name:University of Kansas Medical Center
                     address:
                        name:Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Kenneth R Peterson
               affiliation:
                     name:University of Kansas Medical Center
                     address:
                        name:Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, USA
                        type:PostalAddress
                     type:Organization
                     name:University of Kansas Medical Center
                     address:
                        name:Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, USA
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
         isAccessibleForFree:1
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:Rapid isolation of yeast genomic DNA: Bust n' Grab
      description:Mutagenesis of yeast artificial chromosomes (YACs) often requires analysis of large numbers of yeast clones to obtain correctly targeted mutants. Conventional ways to isolate yeast genomic DNA utilize either glass beads or enzymatic digestion to disrupt yeast cell wall. Using small glass beads is messy, whereas enzymatic digestion of the cells is expensive when many samples need to be analyzed. We sought to develop an easier and faster protocol than the existing methods for obtaining yeast genomic DNA from liquid cultures or colonies on plates. Repeated freeze-thawing of cells in a lysis buffer was used to disrupt the cells and release genomic DNA. Cell lysis was followed by extraction with chloroform and ethanol precipitation of DNA. Two hundred ng – 3 μg of genomic DNA could be isolated from a 1.5 ml overnight liquid culture or from a large colony. Samples were either resuspended directly in a restriction enzyme/RNase coctail mixture for Southern blot hybridization or used for several PCR reactions. We demonstrated the utility of this method by showing an analysis of yeast clones containing a mutagenized human β-globin locus YAC. An efficient, inexpensive method for obtaining yeast genomic DNA from liquid cultures or directly from colonies was developed. This protocol circumvents the use of enzymes or glass beads, and therefore is cheaper and easier to perform when processing large numbers of samples.
      datePublished:2004-04-21T00:00:00Z
      dateModified:2004-04-21T00:00:00Z
      pageStart:1
      pageEnd:6
      sameAs:https://doi.org/10.1186/1472-6750-4-8
      keywords:
         Globin Gene
         Southern Blot Hybridization
         loxP Site
         Yeast Artificial Chromosome
         Yeast Cell Wall
         Applied Microbiology
         Biotechnology
         Biochemical Engineering
         Genetic Engineering
         Plant Breeding/Biotechnology
         Biomedical Engineering/Biotechnology
      image:
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2F1472-6750-4-8/MediaObjects/12896_2004_Article_65_Fig1_HTML.jpg
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2F1472-6750-4-8/MediaObjects/12896_2004_Article_65_Fig2_HTML.jpg
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2F1472-6750-4-8/MediaObjects/12896_2004_Article_65_Fig3_HTML.jpg
      isPartOf:
         name:BMC Biotechnology
         issn:
            1472-6750
         volumeNumber:4
         type:
            Periodical
            PublicationVolume
      publisher:
         name:BioMed Central
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Susanna Harju
            affiliation:
                  name:University of Kansas Medical Center
                  address:
                     name:Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Halyna Fedosyuk
            affiliation:
                  name:University of Kansas Medical Center
                  address:
                     name:Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Kenneth R Peterson
            affiliation:
                  name:University of Kansas Medical Center
                  address:
                     name:Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, USA
                     type:PostalAddress
                  type:Organization
                  name:University of Kansas Medical Center
                  address:
                     name:Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, USA
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      isAccessibleForFree:1
["Periodical","PublicationVolume"]:
      name:BMC Biotechnology
      issn:
         1472-6750
      volumeNumber:4
Organization:
      name:BioMed Central
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:University of Kansas Medical Center
      address:
         name:Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, USA
         type:PostalAddress
      name:University of Kansas Medical Center
      address:
         name:Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, USA
         type:PostalAddress
      name:University of Kansas Medical Center
      address:
         name:Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, USA
         type:PostalAddress
      name:University of Kansas Medical Center
      address:
         name:Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, USA
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Susanna Harju
      affiliation:
            name:University of Kansas Medical Center
            address:
               name:Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, USA
               type:PostalAddress
            type:Organization
      name:Halyna Fedosyuk
      affiliation:
            name:University of Kansas Medical Center
            address:
               name:Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, USA
               type:PostalAddress
            type:Organization
      name:Kenneth R Peterson
      affiliation:
            name:University of Kansas Medical Center
            address:
               name:Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, USA
               type:PostalAddress
            type:Organization
            name:University of Kansas Medical Center
            address:
               name:Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, USA
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, USA
      name:Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, USA
      name:Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, USA
      name:Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, USA

External Links {🔗}(52)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

CDN Services {📦}

Crossref

3.85s.

LINK . SPRINGER . COM {}