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We are analyzing https://link.springer.com/article/10.1007/s13770-022-00477-x.

Title:
Expression of RUNX2/LAPTM5 in the Induction of MC3T3-e1 Mineralization and Its Possible Relationship with Autophagy | Tissue Engineering and Regenerative Medicine
Description:
Background: The study aims to correlate osteogenesis with autophagy during the mineralization induction of MC3T3-e1 through exploring the expression of runt-related transcription factor 2 (RUNX2)/lysosomal-associated transmembrane protein 5 (LAMPT5). Methods: The induction of mineralization in MC3T3-e1 was followed by detecting the expressions of osteogenesis-related indexes such as RUNX2, alkaline phosphatase (ALP), osteocalcin (OCN), and LAPTM5 using RT-qPCR and Western blot from 0 to 14 days. Transmission electron microscope was utilised in visualizing the alterations of autophagosomes, which was followed by immunofluorescence detecting the subcellular localization of autophagy-related index sequestosome 1 (P62) and microtubule-associated protein 1 light 3 (LC3) protein and scrutinising the expression of P62 mRNA and P62 and LC3 proteins. Results: Induction of MC3T3-e1 mineralization demonstrated an increased expression of osteogenesis-related indicators such as RUNX2, ALP, OCN, and LAPTM5 (p < 0.05), as evident from the results of RT-qPCR and Western blot. Meanwhile, the expression of autophagosomes increased one day after mineralization induction and then experienced a gradual decline, and enhanced expression of LC3 protein was noted on days 1–2 of mineralization induction but was then followed by a corresponding reduce. In contrast, a continuous increase was reported in the expression of P62 mRNA and protein, respectively (p < 0.05). Up- and down-regulating RUNX2/LAPTM5 expression alone confirmed the aforementioned results. Conclusion: It was therefore proposed that RUNX2 may be responsible for an early increase and then a gradual decrease in LAPTM5-mediated autophagy through the regulation of its high expression. Meanwhile, increased LAPTM5 expression in osteogenic mineralization presumed that RUNX2/LAPTM5 promoted autophagy and osteogenic expression, which may play a bridging role in the regulation of autophagy and osteogenesis.
Website Age:
28 years and 1 months (reg. 1997-05-29).

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🌠 Phenomenal Traffic: 5M - 10M visitors per month


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Keywords {πŸ”}

article, pubmed, autophagy, google, scholar, cas, expression, central, mineralization, cells, bone, liu, protein, mcte, osteogenic, differentiation, stem, cell, induction, runx, mesenchymal, xing, laptm, china, guangzhou, privacy, cookies, content, research, runxlaptm, fang, osteogenesis, regulation, access, human, springer, information, publish, search, lei, geng, transmembrane, results, role, osteoblasts, biol, osteoblastic, med, res, kim,

Topics {βœ’οΈ}

perk-atf4-chop dependent manner month download article/chapter coordinated time-dependent modulation mir-124-3p/igf2bp1 axis mc3t3-e1 osteoblastic cells osteoblastic mc3t3-e1 cells ampk/akt/mtor signaling article tissue engineering mc3t3-e1 mineralization demonstrated full article pdf runx2/laptm5 promoted autophagy privacy choices/manage cookies eur cells mater osteoblast terminal differentiation osteogenesis-related indexes osteogenesis-related indicators scope lei xing affiliated stomatology hospital natural science foundation regulating runx2/laptm5 expression pingping xu mol med rep regulate osteogenic differentiation yingtao lv ying fang human primary osteoblasts mc3t3-e1 mineralization article xing effectively regulating autophagy osteoblastic lysosome plays bone formation owing kaempferol-induced autophagy regenerative medicine aims oral regenerative medicine cell commun signal cell receptor levels cell biol int related subjects european economic area transmission electron microscope lncrna malat1 targeting pellet culture model fip200 deletion leads ultradistal radius bmd intra-articular delivery southern medical university ethics declarations conflict accepted manuscript version bone miner res activating osteocyte autophagy

Schema {πŸ—ΊοΈ}

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         headline:Expression of RUNX2/LAPTM5 in the Induction of MC3T3-e1 Mineralization and Its Possible Relationship with Autophagy
         description:The study aims to correlate osteogenesis with autophagy during the mineralization induction of MC3T3-e1 through exploring the expression of runt-related transcription factor 2 (RUNX2)/lysosomal-associated transmembrane protein 5 (LAMPT5). The induction of mineralization in MC3T3-e1 was followed by detecting the expressions of osteogenesis-related indexes such as RUNX2, alkaline phosphatase (ALP), osteocalcin (OCN), and LAPTM5 using RT-qPCR and Western blot from 0 to 14Β days. Transmission electron microscope was utilised in visualizing the alterations of autophagosomes, which was followed by immunofluorescence detecting the subcellular localization of autophagy-related index sequestosome 1 (P62) and microtubule-associated protein 1 light 3 (LC3) protein and scrutinising the expression of P62 mRNA and P62 and LC3 proteins. Induction of MC3T3-e1 mineralization demonstrated an increased expression of osteogenesis-related indicators such as RUNX2, ALP, OCN, and LAPTM5 (p &lt; 0.05), as evident from the results of RT-qPCR and Western blot. Meanwhile, the expression of autophagosomes increased one day after mineralization induction and then experienced a gradual decline, and enhanced expression of LC3 protein was noted on days 1–2 of mineralization induction but was then followed by a corresponding reduce. In contrast, a continuous increase was reported in the expression of P62 mRNA and protein, respectively (p &lt; 0.05). Up- and down-regulating RUNX2/LAPTM5 expression alone confirmed the aforementioned results. It was therefore proposed that RUNX2 may be responsible for an early increase and then a gradual decrease in LAPTM5-mediated autophagy through the regulation of its high expression. Meanwhile, increased LAPTM5 expression in osteogenic mineralization presumed that RUNX2/LAPTM5 promoted autophagy and osteogenic expression, which may play a bridging role in the regulation of autophagy and osteogenesis.
         datePublished:2022-09-19T00:00:00Z
         dateModified:2022-09-19T00:00:00Z
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            RUNX2
            LAPTM5
            Osteogenesis
            Autophagy
            Correlativity
            Regenerative Medicine/Tissue Engineering
            Biomedical Engineering and Bioengineering
            Stem Cells
            Cell Biology
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               name:Lei Xing
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                     name:Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine
                     address:
                        name:Department of Dental Implantology, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou, China
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                     address:
                        name:Stomatological Hospital, Southern Medical University, Guangzhou, China
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               name:Yanqin Li
               affiliation:
                     name:Chinese Academy of Sciences
                     address:
                        name:South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China
                        type:PostalAddress
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               type:Person
               name:Wenhao Li
               affiliation:
                     name:Southern Medical University
                     address:
                        name:Stomatological Hospital, Southern Medical University, Guangzhou, China
                        type:PostalAddress
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               name:Rong Liu
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                     address:
                        name:Department of Dental Implantology, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou, China
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                        name:Department of Dental Implantology, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou, China
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      headline:Expression of RUNX2/LAPTM5 in the Induction of MC3T3-e1 Mineralization and Its Possible Relationship with Autophagy
      description:The study aims to correlate osteogenesis with autophagy during the mineralization induction of MC3T3-e1 through exploring the expression of runt-related transcription factor 2 (RUNX2)/lysosomal-associated transmembrane protein 5 (LAMPT5). The induction of mineralization in MC3T3-e1 was followed by detecting the expressions of osteogenesis-related indexes such as RUNX2, alkaline phosphatase (ALP), osteocalcin (OCN), and LAPTM5 using RT-qPCR and Western blot from 0 to 14Β days. Transmission electron microscope was utilised in visualizing the alterations of autophagosomes, which was followed by immunofluorescence detecting the subcellular localization of autophagy-related index sequestosome 1 (P62) and microtubule-associated protein 1 light 3 (LC3) protein and scrutinising the expression of P62 mRNA and P62 and LC3 proteins. Induction of MC3T3-e1 mineralization demonstrated an increased expression of osteogenesis-related indicators such as RUNX2, ALP, OCN, and LAPTM5 (p &lt; 0.05), as evident from the results of RT-qPCR and Western blot. Meanwhile, the expression of autophagosomes increased one day after mineralization induction and then experienced a gradual decline, and enhanced expression of LC3 protein was noted on days 1–2 of mineralization induction but was then followed by a corresponding reduce. In contrast, a continuous increase was reported in the expression of P62 mRNA and protein, respectively (p &lt; 0.05). Up- and down-regulating RUNX2/LAPTM5 expression alone confirmed the aforementioned results. It was therefore proposed that RUNX2 may be responsible for an early increase and then a gradual decrease in LAPTM5-mediated autophagy through the regulation of its high expression. Meanwhile, increased LAPTM5 expression in osteogenic mineralization presumed that RUNX2/LAPTM5 promoted autophagy and osteogenic expression, which may play a bridging role in the regulation of autophagy and osteogenesis.
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      dateModified:2022-09-19T00:00:00Z
      pageStart:1223
      pageEnd:1235
      sameAs:https://doi.org/10.1007/s13770-022-00477-x
      keywords:
         RUNX2
         LAPTM5
         Osteogenesis
         Autophagy
         Correlativity
         Regenerative Medicine/Tissue Engineering
         Biomedical Engineering and Bioengineering
         Stem Cells
         Cell Biology
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      author:
            name:Lei Xing
            affiliation:
                  name:Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine
                  address:
                     name:Department of Dental Implantology, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou, China
                     type:PostalAddress
                  type:Organization
                  name:Southern Medical University
                  address:
                     name:Stomatological Hospital, Southern Medical University, Guangzhou, China
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            name:Yanqin Li
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                  address:
                     name:South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China
                     type:PostalAddress
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            type:Person
            name:Wenhao Li
            affiliation:
                  name:Southern Medical University
                  address:
                     name:Stomatological Hospital, Southern Medical University, Guangzhou, China
                     type:PostalAddress
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            name:Rong Liu
            affiliation:
                  name:Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine
                  address:
                     name:Department of Dental Implantology, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou, China
                     type:PostalAddress
                  type:Organization
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            name:Yuanming Geng
            affiliation:
                  name:Southern Medical University
                  address:
                     name:Zhujiang Hospital, Southern Medical University, Guangzhou, China
                     type:PostalAddress
                  type:Organization
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                  name:Southern Medical University
                  address:
                     name:Stomatological Hospital, Southern Medical University, Guangzhou, China
                     type:PostalAddress
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            type:Person
            name:Yu Qiao
            affiliation:
                  name:Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine
                  address:
                     name:Department of Dental Implantology, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou, China
                     type:PostalAddress
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            type:Person
            name:Jianwen Li
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                  name:Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine
                  address:
                     name:Department of Dental Implantology, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou, China
                     type:PostalAddress
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            name:Yingtao Lv
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                  address:
                     name:Stomatological Hospital, Southern Medical University, Guangzhou, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Ying Fang
            affiliation:
                  name:Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine
                  address:
                     name:Department of Dental Implantology, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou, China
                     type:PostalAddress
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            name:Pingping Xu
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            address:
               name:Stomatological Hospital, Southern Medical University, Guangzhou, China
               type:PostalAddress
            type:Organization
      name:Rong Liu
      affiliation:
            name:Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine
            address:
               name:Department of Dental Implantology, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou, China
               type:PostalAddress
            type:Organization
      name:Yuanming Geng
      affiliation:
            name:Southern Medical University
            address:
               name:Zhujiang Hospital, Southern Medical University, Guangzhou, China
               type:PostalAddress
            type:Organization
      name:Weiqun Ma
      affiliation:
            name:Southern Medical University
            address:
               name:Stomatological Hospital, Southern Medical University, Guangzhou, China
               type:PostalAddress
            type:Organization
      name:Yu Qiao
      affiliation:
            name:Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine
            address:
               name:Department of Dental Implantology, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou, China
               type:PostalAddress
            type:Organization
      name:Jianwen Li
      affiliation:
            name:Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine
            address:
               name:Department of Dental Implantology, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou, China
               type:PostalAddress
            type:Organization
      name:Yingtao Lv
      affiliation:
            name:Southern Medical University
            address:
               name:Stomatological Hospital, Southern Medical University, Guangzhou, China
               type:PostalAddress
            type:Organization
      name:Ying Fang
      affiliation:
            name:Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine
            address:
               name:Department of Dental Implantology, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou, China
               type:PostalAddress
            type:Organization
      email:[email protected]
      name:Pingping Xu
      url:http://orcid.org/0000-0002-3589-7394
      affiliation:
            name:Southern Medical University
            address:
               name:Stomatological Hospital, Southern Medical University, Guangzhou, China
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Department of Dental Implantology, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou, China
      name:Stomatological Hospital, Southern Medical University, Guangzhou, China
      name:South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China
      name:Stomatological Hospital, Southern Medical University, Guangzhou, China
      name:Department of Dental Implantology, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou, China
      name:Zhujiang Hospital, Southern Medical University, Guangzhou, China
      name:Stomatological Hospital, Southern Medical University, Guangzhou, China
      name:Department of Dental Implantology, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou, China
      name:Department of Dental Implantology, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou, China
      name:Stomatological Hospital, Southern Medical University, Guangzhou, China
      name:Department of Dental Implantology, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou, China
      name:Stomatological Hospital, Southern Medical University, Guangzhou, China
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