We are analyzing https://link.springer.com/article/10.1007/s13770-020-00266-4.

Title:
Human Hair Outer Root Sheath Cells and Platelet-Lysis Exosomes Promote Hair Inductivity of Dermal Papilla Cell | Tissue Engineering and Regenerative Medicine
Description:
BACKGROUND: Hair loss is a prevalent medical problem in both men and women. Maintaining the potential hair inductivity of dermal papilla cells (DPCs) during cell culture is the main factor in hair follicle morphogenesis and regeneration. The present study was conducted to compare the effects of different concentrations of human hair outer root sheath cell (HHORSC) and platelet lysis (PL) exosomes to maintain hair inductivity of the human dermal papilla cells (hDPCs). METHODS: In this study, hDPCs and HHORSCs were isolated from healthy hair samples. Specific markers of hDPCs (versican, α-SMA) and HHORSCs (K15) were evaluated using flow cytometric and immunocytochemical techniques. The exosomes were isolated from HHORSCs and PL with ultracentrifugation technique. Western blot was used to detect specific markers of HHORSCs and PL exosomes. Particle size and distribution of the exosomes were analyzed by NanoSight dynamic light NanoSight Dynamic Light Scattering. Different methods such as proliferation test (MTS assay), migration test (Transwell assay) were used to evaluate the effects of different concentrations of exosomes (2,550,100 µg/ml) derived from HHORSC and PL on hDPCs. Expression of specific genes in the hair follicle inductivity, including ALP, versican and α-SMA were also evaluated using real time-PCR. RESULTS: The flow cytometry of the specific cytoplasmic markers of the hDPCs and HHORSCs showed expression of versican (77%), α-SMA (55.2%) and K15 (73.2%). The result of particle size and distribution of the exosomes were analyzed by NanoSight dynamic light NanoSight Dynamic Light Scattering, which revealed the majority of HHORSC and PL exosomes were 30–150 nm. For 100 µg/ml of HHORSC exosomes, the expressions of ALP, versican and α-SMA proteins respectively increased by a factor of 2.1, 1.7and 1.3 compared to those in the control group. CONCLUSION: In summary, we applied HHORSC exosomes as a new method to support hair inductivity of dermal papilla cells and improve the outcome for the treatment of hair loss.
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Keywords {🔍}

pubmed, article, hair, google, scholar, cells, stem, central, cas, cell, exosomes, dermal, human, papilla, follicle, int, growth, research, inductivity, loss, access, mesenchymal, alopecia, skin, sci, surg, regenerative, regeneration, privacy, cookies, content, medicine, hhorsc, hdpcs, hhorscs, treatment, park, dermatol, mol, res, tehran, supplementary, information, publish, search, tissue, outer, root, sheath, nilforoushzadeh,

Topics {✒️}

suppressing wnt/beta-catenin activation month download article/chapter adipose-derived stem cells follicular unit extraction follicular β-catenin signaling mesenchymal stem cells human hair follicles cell-free therapeutic strategies dermal papilla cells hair-growth-promoting effect wound healing article tissue engineering support hair inductivity dermal papilla cell human cells involved hair follicle inductivity stem cells int autologous platelet-rich plasma human hair follicle stem cell investig telogen mouse skin maintain hair inductivity promotes hair growth potential hair inductivity positive keratinocyte cells full article pdf stem cell biology related subjects platelet-derived therapies article nilforoushzadeh promoting hair growth stem cells hair growth stimulated hair follicle morphogenesis hair follicle generation privacy choices/manage cookies hair-growth potential healthy hair samples hair germ model hair follicle development hair regeneration therapy hair follicle regeneration activating notch signaling check access instant access regenerative medicine aims ehsan taghiabadi mol biol cell platelet-rich plasma platelet rich plasma

Questions {❓}

Platelet Rich Plasma in Androgenic Alopecia: Where do we Stand?

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         description:Hair loss is a prevalent medical problem in both men and women. Maintaining the potential hair inductivity of dermal papilla cells (DPCs) during cell culture is the main factor in hair follicle morphogenesis and regeneration. The present study was conducted to compare the effects of different concentrations of human hair outer root sheath cell (HHORSC) and platelet lysis (PL) exosomes to maintain hair inductivity of the human dermal papilla cells (hDPCs). In this study, hDPCs and HHORSCs were isolated from healthy hair samples. Specific markers of hDPCs (versican, α-SMA) and HHORSCs (K15) were evaluated using flow cytometric and immunocytochemical techniques. The exosomes were isolated from HHORSCs and PL with ultracentrifugation technique. Western blot was used to detect specific markers of HHORSCs and PL exosomes. Particle size and distribution of the exosomes were analyzed by NanoSight dynamic light NanoSight Dynamic Light Scattering. Different methods such as proliferation test (MTS assay), migration test (Transwell assay) were used to evaluate the effects of different concentrations of exosomes (2,550,100 µg/ml) derived from HHORSC and PL on hDPCs. Expression of specific genes in the hair follicle inductivity, including ALP, versican and α-SMA were also evaluated using real time-PCR. The flow cytometry of the specific cytoplasmic markers of the hDPCs and HHORSCs showed expression of versican (77%), α-SMA (55.2%) and K15 (73.2%). The result of particle size and distribution of the exosomes were analyzed by NanoSight dynamic light NanoSight Dynamic Light Scattering, which revealed the majority of HHORSC and PL exosomes were 30–150 nm. For 100 µg/ml of HHORSC exosomes, the expressions of ALP, versican and α-SMA proteins respectively increased by a factor of 2.1, 1.7and 1.3 compared to those in the control group. In summary, we applied HHORSC exosomes as a new method to support hair inductivity of dermal papilla cells and improve the outcome for the treatment of hair loss.
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