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Title:
Metabolic interventions improve HBV envelope-specific T-cell responses in patients with chronic hepatitis B | Hepatology International
Description:
Background Restoration of HBV-specific T cell immunity is a promising approach for the functional cure of chronic Hepatitis B (CHB), necessitating the development of valid assays to boost and monitor HBV-specific T cell responses in patients with CHB. Methods We analyzed hepatitis B virus (HBV) core- and envelope (env)-specific T cell responses using in vitro expanded peripheral blood mononuclear cells (PBMCs) from patients with CHB exhibiting different immunological phases, including immune tolerance (IT), immune activation (IA), inactive carrier (IC), and HBeAg-negative hepatitis (ENEG). Additionally, we evaluated the effects of metabolic interventions, including mitochondria-targeted antioxidants (MTA), polyphenolic compounds, and ACAT inhibitors (iACAT), on HBV-specific T-cell functionality. Results We found that HBV core- and env-specific T cell responses were finely coordinated and more profound in IC and ENEG than in the IT and IA stages. HBV env-specific T cells were more dysfunctional but prone to respond to metabolic interventions using MTA, iACAT, and polyphenolic compounds than HBV core-specific T-cells. The responsiveness of HBV env-specific T cells to metabolic interventions can be predicted by the eosinophil (EO) count and the coefficient of variation of red blood cell distribution width (RDW-CV). Conclusion These findings may provide valuable information for metabolically invigorating HBV-specific T-cells to treat CHB. Graphical abstract
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Keywords {🔍}
hbv, tcells, hbvspecific, pubmed, tcell, responses, patients, article, envspecific, hepatitis, google, scholar, cells, metabolic, chronic, cas, cell, infection, levels, fig, interventions, chb, core, hbsag, virus, corespecific, elispot, ifnγ, clinical, functional, immune, response, aim, central, mta, stages, viral, function, study, hbeag, positive, treatments, hepatol, cure, eneg, antiviral, treatment, correlation, showed, data,
Topics {✒️}
hbv-specific cd8 + t-cell responses restoring hbv-specific cd8 + t-cells including mitochondria-targeted antioxidants hbv-specific t-cell responses hbv-specific t-cell functionality env-specific t-cell responses env-specific t-cell response core-specific t-cell responses cd8 + t-cell-mediated effects hbv-specific t-cell function env-specific t-cell reactivity hbv-specific cd8 + t-cells t-cell-directed therapy development specific ifn-γ-secreting cells env-specific cd8 + t-cells env-specific t-cells tended hbv env-specific t-cells core-specific cd8 + t-cells core-specific cd8 + t-cells [22 measure hbv-specific t-cells hbv core-specific t-cells functional hbv-specific t-cells env-specific ifn-γ + sfu specific t-cell responses core-specific ifn-γ + sfu delayed t-cell response t-cell responses targeting exhausted hbv-specific cd8 targeting t-cell metabolism t-cell-based therapies polymerase-specific t-cells article download pdf specific t-cell dysfunction hbv-specific t-cells hbv-specific t-cells [32 cd8 + t-cells recognizing antigen-specific t-cells fu-sheng wang env-specific t-cells env-specific t-cells [20 vivo t-cell responses core-specific t-cells provide long-term benefits ifn-γ + sfu produced functional hbv-specific cd8 sterile phosphate-buffered saline anti-ifn-γ af647 long-term nas resulted stop long-term treatment ifn-γ + sfu derived
Questions {❓}
- How to achieve functional cure of HBV: stopping NUCs, adding interferon or new drug development?
- Restoration of a functional antiviral immune response to chronic HBV infection by reducing viral antigen load: if not sufficient, is it necessary?
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headline:Metabolic interventions improve HBV envelope-specific T-cell responses in patients with chronic hepatitis B
description:Restoration of HBV-specific T cell immunity is a promising approach for the functional cure of chronic Hepatitis B (CHB), necessitating the development of valid assays to boost and monitor HBV-specific T cell responses in patients with CHB. We analyzed hepatitis B virus (HBV) core- and envelope (env)-specific T cell responses using in vitro expanded peripheral blood mononuclear cells (PBMCs) from patients with CHB exhibiting different immunological phases, including immune tolerance (IT), immune activation (IA), inactive carrier (IC), and HBeAg-negative hepatitis (ENEG). Additionally, we evaluated the effects of metabolic interventions, including mitochondria-targeted antioxidants (MTA), polyphenolic compounds, and ACAT inhibitors (iACAT), on HBV-specific T-cell functionality. We found that HBV core- and env-specific T cell responses were finely coordinated and more profound in IC and ENEG than in the IT and IA stages. HBV env-specific T cells were more dysfunctional but prone to respond to metabolic interventions using MTA, iACAT, and polyphenolic compounds than HBV core-specific T-cells. The responsiveness of HBV env-specific T cells to metabolic interventions can be predicted by the eosinophil (EO) count and the coefficient of variation of red blood cell distribution width (RDW-CV). These findings may provide valuable information for metabolically invigorating HBV-specific T-cells to treat CHB.
datePublished:2023-03-28T00:00:00Z
dateModified:2023-03-28T00:00:00Z
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HBV
T-cell responses
Core
Envelope
Metabolic interventions
Mitochondria-targeted antioxidants
Polyphenolic compounds
ACAT inhibitors
Eosinophil
RDW-CV
Hepatology
Colorectal Surgery
Surgery
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headline:Metabolic interventions improve HBV envelope-specific T-cell responses in patients with chronic hepatitis B
description:Restoration of HBV-specific T cell immunity is a promising approach for the functional cure of chronic Hepatitis B (CHB), necessitating the development of valid assays to boost and monitor HBV-specific T cell responses in patients with CHB. We analyzed hepatitis B virus (HBV) core- and envelope (env)-specific T cell responses using in vitro expanded peripheral blood mononuclear cells (PBMCs) from patients with CHB exhibiting different immunological phases, including immune tolerance (IT), immune activation (IA), inactive carrier (IC), and HBeAg-negative hepatitis (ENEG). Additionally, we evaluated the effects of metabolic interventions, including mitochondria-targeted antioxidants (MTA), polyphenolic compounds, and ACAT inhibitors (iACAT), on HBV-specific T-cell functionality. We found that HBV core- and env-specific T cell responses were finely coordinated and more profound in IC and ENEG than in the IT and IA stages. HBV env-specific T cells were more dysfunctional but prone to respond to metabolic interventions using MTA, iACAT, and polyphenolic compounds than HBV core-specific T-cells. The responsiveness of HBV env-specific T cells to metabolic interventions can be predicted by the eosinophil (EO) count and the coefficient of variation of red blood cell distribution width (RDW-CV). These findings may provide valuable information for metabolically invigorating HBV-specific T-cells to treat CHB.
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HBV
T-cell responses
Core
Envelope
Metabolic interventions
Mitochondria-targeted antioxidants
Polyphenolic compounds
ACAT inhibitors
Eosinophil
RDW-CV
Hepatology
Colorectal Surgery
Surgery
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name:Senior Department of Infectious Diseases, The Fifth Medical Center of Chinese PLA General Hospital, Beijing, China
type:PostalAddress
type:Organization
name:Si-Yuan Chen
affiliation:
name:The Fifth Medical Center of Chinese PLA General Hospital
address:
name:Senior Department of Infectious Diseases, The Fifth Medical Center of Chinese PLA General Hospital, Beijing, China
type:PostalAddress
type:Organization
name:Xing Fan
affiliation:
name:The Fifth Medical Center of Chinese PLA General Hospital
address:
name:Senior Department of Infectious Diseases, The Fifth Medical Center of Chinese PLA General Hospital, Beijing, China
type:PostalAddress
type:Organization
name:Jin-Wen Song
affiliation:
name:The Fifth Medical Center of Chinese PLA General Hospital
address:
name:Senior Department of Infectious Diseases, The Fifth Medical Center of Chinese PLA General Hospital, Beijing, China
type:PostalAddress
type:Organization
name:Yan-Mei Jiao
affiliation:
name:The Fifth Medical Center of Chinese PLA General Hospital
address:
name:Senior Department of Infectious Diseases, The Fifth Medical Center of Chinese PLA General Hospital, Beijing, China
type:PostalAddress
type:Organization
name:Ruonan Xu
affiliation:
name:The Fifth Medical Center of Chinese PLA General Hospital
address:
name:Senior Department of Infectious Diseases, The Fifth Medical Center of Chinese PLA General Hospital, Beijing, China
type:PostalAddress
type:Organization
name:Ji-Yuan Zhang
affiliation:
name:The Fifth Medical Center of Chinese PLA General Hospital
address:
name:Senior Department of Infectious Diseases, The Fifth Medical Center of Chinese PLA General Hospital, Beijing, China
type:PostalAddress
type:Organization
name:Cheng Zhen
affiliation:
name:The Fifth Medical Center of Chinese PLA General Hospital
address:
name:Senior Department of Infectious Diseases, The Fifth Medical Center of Chinese PLA General Hospital, Beijing, China
type:PostalAddress
type:Organization
name:Chun-Bao Zhou
affiliation:
name:The Fifth Medical Center of Chinese PLA General Hospital
address:
name:Senior Department of Infectious Diseases, The Fifth Medical Center of Chinese PLA General Hospital, Beijing, China
type:PostalAddress
type:Organization
name:Jin-Hong Yuan
affiliation:
name:The Fifth Medical Center of Chinese PLA General Hospital
address:
name:Senior Department of Infectious Diseases, The Fifth Medical Center of Chinese PLA General Hospital, Beijing, China
type:PostalAddress
type:Organization
name:Ming Shi
affiliation:
name:The Fifth Medical Center of Chinese PLA General Hospital
address:
name:Senior Department of Infectious Diseases, The Fifth Medical Center of Chinese PLA General Hospital, Beijing, China
type:PostalAddress
type:Organization
name:Fu-Sheng Wang
url:http://orcid.org/0000-0002-8043-6685
affiliation:
name:University of Chinese Academy of Sciences
address:
name:Savaid Medical School, University of Chinese Academy of Sciences, Beijing, China
type:PostalAddress
type:Organization
name:The Fifth Medical Center of Chinese PLA General Hospital
address:
name:Senior Department of Infectious Diseases, The Fifth Medical Center of Chinese PLA General Hospital, Beijing, China
type:PostalAddress
type:Organization
email:[email protected]
name:Chao Zhang
affiliation:
name:The Fifth Medical Center of Chinese PLA General Hospital
address:
name:Senior Department of Infectious Diseases, The Fifth Medical Center of Chinese PLA General Hospital, Beijing, China
type:PostalAddress
type:Organization
email:[email protected]
PostalAddress:
name:Savaid Medical School, University of Chinese Academy of Sciences, Beijing, China
name:Senior Department of Infectious Diseases, The Fifth Medical Center of Chinese PLA General Hospital, Beijing, China
name:Senior Department of Infectious Diseases, The Fifth Medical Center of Chinese PLA General Hospital, Beijing, China
name:Senior Department of Infectious Diseases, The Fifth Medical Center of Chinese PLA General Hospital, Beijing, China
name:Senior Department of Infectious Diseases, The Fifth Medical Center of Chinese PLA General Hospital, Beijing, China
name:Senior Department of Infectious Diseases, The Fifth Medical Center of Chinese PLA General Hospital, Beijing, China
name:Senior Department of Infectious Diseases, The Fifth Medical Center of Chinese PLA General Hospital, Beijing, China
name:Senior Department of Infectious Diseases, The Fifth Medical Center of Chinese PLA General Hospital, Beijing, China
name:Senior Department of Infectious Diseases, The Fifth Medical Center of Chinese PLA General Hospital, Beijing, China
name:Senior Department of Infectious Diseases, The Fifth Medical Center of Chinese PLA General Hospital, Beijing, China
name:Senior Department of Infectious Diseases, The Fifth Medical Center of Chinese PLA General Hospital, Beijing, China
name:Senior Department of Infectious Diseases, The Fifth Medical Center of Chinese PLA General Hospital, Beijing, China
name:Senior Department of Infectious Diseases, The Fifth Medical Center of Chinese PLA General Hospital, Beijing, China
name:Senior Department of Infectious Diseases, The Fifth Medical Center of Chinese PLA General Hospital, Beijing, China
name:Senior Department of Infectious Diseases, The Fifth Medical Center of Chinese PLA General Hospital, Beijing, China
name:Senior Department of Infectious Diseases, The Fifth Medical Center of Chinese PLA General Hospital, Beijing, China
name:Senior Department of Infectious Diseases, The Fifth Medical Center of Chinese PLA General Hospital, Beijing, China
name:Senior Department of Infectious Diseases, The Fifth Medical Center of Chinese PLA General Hospital, Beijing, China
name:Senior Department of Infectious Diseases, The Fifth Medical Center of Chinese PLA General Hospital, Beijing, China
name:Savaid Medical School, University of Chinese Academy of Sciences, Beijing, China
name:Senior Department of Infectious Diseases, The Fifth Medical Center of Chinese PLA General Hospital, Beijing, China
name:Senior Department of Infectious Diseases, The Fifth Medical Center of Chinese PLA General Hospital, Beijing, China
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