We are analyzing https://link.springer.com/article/10.1007/s12035-017-0679-z.

Title:
LINE-1 Hypermethylation in Serum Cell-Free DNA of Relapsing Remitting Multiple Sclerosis Patients | Molecular Neurobiology
Description:
Concentrations of cell-free DNA (cfDNA) circulating in blood and its epigenetic variation, such as DNA methylation, may provide useful diagnostic or prognostic information. Long interspersed nuclear element-1 (LINE-1) constitutes approximately 20% of the human genome and its 5’UTR region is CpG rich. Due to its wide distribution, the methylation level of the 5’UTR of LINE-1 can serve as a surrogate marker of global genomic DNA methylation. The aim of the current study was to investigate whether the methylation status of LINE-1 elements in serum cell-free DNA differs between relapsing remitting multiple sclerosis (RRMS) patients and healthy control subjects (CTR). Serum DNA samples of 6 patients and 6 controls were subjected to bisulfite sequencing. The results showed that the methylation level varies among distinct CpG sites in the 5’UTR of LINE-1 repeats and revealed differences in the methylation state of specific sites in this element between patients and controls. The latter differences were largely due to CpG sites in the L1PA2 subfamily, which were more frequently methylated in the RRMS patients than in the CTR group, whereas such differences were not observed in the L1HS subfamily. These data were verified by quantitative PCR using material from 18 patients and 18 control subjects. The results confirmed that the methylation level of a subset of the CpG sites within the LINE-1 promoter is elevated in DNA from RRMS patients in comparison with CTR. The present data suggest that the methylation status of CpG sites of LINE repeats could be a basis for development of diagnostic or prognostic tests.
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Keywords {🔍}

methylation, cpg, line, dna, pubmed, sites, article, google, scholar, rrms, lpa, patients, levels, analysis, elements, cas, cfdna, ctr, lhs, site, human, central, multiple, sclerosis, pcr, qpcr, genome, samples, bisulfite, methylated, serum, differences, individual, level, status, repetitive, study, healthy, controls, sequencing, results, primer, data, subjects, promoter, blood, subfamily, observed, cancer, fig,

Topics {✒️}

article download pdf cell-free dna comprises bisulfite-treated dna served serum cell-free dna unmethylated bisulfite-treated dna cell-free circulating dna central nervous system full-length line-1 elements including relapsing remitting methylation-specific quantitative pcr primer set l1f/lr l1f/lr primer set kazazian hh jr multiple sclerosis center web server issue polymerase chain reaction multiple sclerosis patients interspersed repetitive sequences open reading frames high resolution image cell-free dna full access completely unmethylated dna human-specific l1 elements privacy choices/manage cookies article dunaeva taq dna polymerase dna size markers lower expression levels bisulfite-treated dna global dna methylation quantitative pcr analysis repetitive element hypermethylation molecular life sciences viral quantitative pcr epigenetic variations contributed line-1 areas free sodium bisulfite treatment dna sequence analysis restriction enzyme analysis fully methylated dna assumption-free analysis epitect bisulfite kit multiple sclerosis epitect control dna higher methylation levels agarose gel electrophoresis previously published studies repetitive dna elements radboud medical center

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WebPage:
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         headline:LINE-1 Hypermethylation in Serum Cell-Free DNA of Relapsing Remitting Multiple Sclerosis Patients
         description:Concentrations of cell-free DNA (cfDNA) circulating in blood and its epigenetic variation, such as DNA methylation, may provide useful diagnostic or prognostic information. Long interspersed nuclear element-1 (LINE-1) constitutes approximately 20% of the human genome and its 5’UTR region is CpG rich. Due to its wide distribution, the methylation level of the 5’UTR of LINE-1 can serve as a surrogate marker of global genomic DNA methylation. The aim of the current study was to investigate whether the methylation status of LINE-1 elements in serum cell-free DNA differs between relapsing remitting multiple sclerosis (RRMS) patients and healthy control subjects (CTR). Serum DNA samples of 6 patients and 6 controls were subjected to bisulfite sequencing. The results showed that the methylation level varies among distinct CpG sites in the 5’UTR of LINE-1 repeats and revealed differences in the methylation state of specific sites in this element between patients and controls. The latter differences were largely due to CpG sites in the L1PA2 subfamily, which were more frequently methylated in the RRMS patients than in the CTR group, whereas such differences were not observed in the L1HS subfamily. These data were verified by quantitative PCR using material from 18 patients and 18 control subjects. The results confirmed that the methylation level of a subset of the CpG sites within the LINE-1 promoter is elevated in DNA from RRMS patients in comparison with CTR. The present data suggest that the methylation status of CpG sites of LINE repeats could be a basis for development of diagnostic or prognostic tests.
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         dateModified:2017-07-13T00:00:00Z
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ScholarlyArticle:
      headline:LINE-1 Hypermethylation in Serum Cell-Free DNA of Relapsing Remitting Multiple Sclerosis Patients
      description:Concentrations of cell-free DNA (cfDNA) circulating in blood and its epigenetic variation, such as DNA methylation, may provide useful diagnostic or prognostic information. Long interspersed nuclear element-1 (LINE-1) constitutes approximately 20% of the human genome and its 5’UTR region is CpG rich. Due to its wide distribution, the methylation level of the 5’UTR of LINE-1 can serve as a surrogate marker of global genomic DNA methylation. The aim of the current study was to investigate whether the methylation status of LINE-1 elements in serum cell-free DNA differs between relapsing remitting multiple sclerosis (RRMS) patients and healthy control subjects (CTR). Serum DNA samples of 6 patients and 6 controls were subjected to bisulfite sequencing. The results showed that the methylation level varies among distinct CpG sites in the 5’UTR of LINE-1 repeats and revealed differences in the methylation state of specific sites in this element between patients and controls. The latter differences were largely due to CpG sites in the L1PA2 subfamily, which were more frequently methylated in the RRMS patients than in the CTR group, whereas such differences were not observed in the L1HS subfamily. These data were verified by quantitative PCR using material from 18 patients and 18 control subjects. The results confirmed that the methylation level of a subset of the CpG sites within the LINE-1 promoter is elevated in DNA from RRMS patients in comparison with CTR. The present data suggest that the methylation status of CpG sites of LINE repeats could be a basis for development of diagnostic or prognostic tests.
      datePublished:2017-07-13T00:00:00Z
      dateModified:2017-07-13T00:00:00Z
      pageStart:4681
      pageEnd:4688
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      keywords:
         Multiple sclerosis
         LINE-1
         CpG
         DNA methylation
         Cell-free DNA
         Neurosciences
         Neurobiology
         Cell Biology
         Neurology
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               type:PostalAddress
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      email:[email protected]
      name:Merel Derksen
      affiliation:
            name:Radboud University Nijmegen
            address:
               name:Department of Biomolecular Chemistry, Institute for Molecules and Materials and Radboud Institute for Molecular Life Sciences, Radboud University Nijmegen, Nijmegen, The Netherlands
               type:PostalAddress
            type:Organization
      name:Ger J. M. Pruijn
      affiliation:
            name:Radboud University Nijmegen
            address:
               name:Department of Biomolecular Chemistry, Institute for Molecules and Materials and Radboud Institute for Molecular Life Sciences, Radboud University Nijmegen, Nijmegen, The Netherlands
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      name:Department of Biomolecular Chemistry, Institute for Molecules and Materials and Radboud Institute for Molecular Life Sciences, Radboud University Nijmegen, Nijmegen, The Netherlands
      name:Department of Biomolecular Chemistry, Institute for Molecules and Materials and Radboud Institute for Molecular Life Sciences, Radboud University Nijmegen, Nijmegen, The Netherlands

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