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month download article/chapter n-cadherin/fgfr promotes metastasis hypoxia-induced lysyl oxidase hypoxia-inducible factor-1α lacking hypoxia-mediated downregulation hif-1alpha promotes metastasis epithelial–mesenchymal transition epithelial-mesenchymal transition full article pdf laryngeal carcinoma tissue key regulatory factor hypoxic hep-2 cells key research program epithelial–mesenchymal plasticity hypoxia-inducible factors cell proliferation regulation squamous cell carcinomas laryngeal cancer cells privacy choices/manage cookies mir-210 links hypoxia lung adenocarcinoma cells e-cadherin expression hepatocellular carcinoma cells huang lj article zuo breast cancer cells li mao-yu human laryngeal cancer human laryngeal cancer cancer stem cell side population cells circulating tumour cells stem/progenitor cell epsin-3 inhibits migration patient survival rate breast cancer res glucose transporter-1 expression mesenchymal transition mesenchymal transition zhangjiajie city hospital clin cancer res hypoxia promotes nat cell biol mol biol cell zhaoyang luo endocr relat cancer human laryngocarcinoma cancer mol cancer res emt-related proteins article log

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         description:The purpose of this study is to explore the role of hypoxia on the invasion and metastasis of laryngeal carcinoma. The invasion and migration ability of laryngeal cancer SCC10A cell was detected by transwell assay. Western blot was applied to analyze the expression of EMT-related proteins. Fifty-seven samples from postoperative patients with laryngeal cancer were collected to study. Immunohistochemistry was used to examine the expression of GLUT-1 and EMT-related proteins (Vim, E-cad, N-cad) in normal laryngeal squamous epithelial tissue, laryngeal cancer adjacent tissues and laryngeal squamous cell carcinoma tissues. Hypoxia promoted laryngeal cancer cell invasion and migration. Hypoxia also enhanced the expression of GLUT-1, vimentin and N-cad, which exist statistically significant correlation with the clinical staging and lymph node metastases (P < 0.05). The expression of GLUT-1 is positively correlated with Vim and N-cad expression in laryngeal squamous cell carcinoma tissues, but negatively correlated with E-cad expression. The patient survival rate with the positive expression of GLUT-1, Vim and N-cad becomes much shorter compared with those with negative expression of GLUT-1, Vim and N-cad (P < 0.05). Hypoxia promoted laryngeal cancer cell invasion and migration via EMT.
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      headline:Hypoxia promotes the invasion and metastasis of laryngeal cancer cells via EMT
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