We are analyzing https://link.springer.com/article/10.1007/s11306-010-0249-0.

Title:
Stable isotope resolved metabolomics of lung cancer in a SCID mouse model | Metabolomics
Description:
We have determined the time course of [U-13C]-glucose utilization and transformations in SCID mice via bolus injection of the tracer in the tail vein. Incorporation of 13C into metabolites extracted from mouse blood plasma and several tissues (lung, heart, brain, liver, kidney, and skeletal muscle) were profiled by NMR and GC–MS, which helped ascertain optimal sampling times for different target tissues. We found that the time for overall optimal 13C incorporation into tissue was 15–20 min but with substantial differences in 13C labeling patterns of various organs that reflected their specific metabolism. Using this stable isotope resolved metabolomics (SIRM) approach, we have compared the 13C metabolite profile of the lungs in the same mouse with or without an orthotopic lung tumor xenograft established from human PC14PE6 lung adenocarcinoma cells. The 13C metabolite profile shows considerable differences in [U-13C]-glucose transformations between the two lung tissues, demonstrating the feasibility of applying SIRM to investigate metabolic networks of human cancer xenograft in the mouse model.
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Keywords {🔍}

article, google, scholar, cas, pubmed, cancer, metabolomics, fan, lane, nmr, journal, higashi, metabolism, lung, metabolic, research, stable, human, mouse, vivo, magnetic, resonance, analysis, isotope, model, models, medicine, brain, sirm, privacy, cookies, content, resolved, scid, teresa, liver, cells, access, cell, national, louisville, publish, search, andrew, richard, ucglucose, tumor, xenograft, glickson, spectroscopy,

Topics {✒️}

n-methyl-n-[tert-butyl-dimethylsilyl]trifluoroacetamide nsclc month download article/chapter [u-13c6]-d-glucose tracer gas chromatography-mass spectra stable isotope tracers metabolomics-edited transcriptomics analysis [u-13c]-glucose utilization related subjects isotopomer-based metabolomic analysis rna ribose turnover article metabolomics aims [u-13c]-glucose transformations mass spectrometry full article pdf patient-derived models 13c metabolite profile privacy choices/manage cookies human cancer xenograft clinical cancer metabolomics glial-neuronal metabolism glial metabolism studied human tumor models scid mouse model mouse xenograft models lactate methyl signal investigate metabolic networks specific metabolism clinical cancer research regulatory environmental metabolomics shumaker research building vitro metabolomic analysis maximum metabolic rate breast cancer research profiling substrate fluxes crude cell extracts human glioma cells scaling metabolic rate komen foundation bctr0503648 [u-c-13]glucose national cancer institute structure-based profiling human cancer therapeutics optimal 13c incorporation 13c labeling patterns human cerebral cortex glutamine metabolism european economic area interrogating drug action 2′dimethylsilapentane-5-sulfonate mtbstfa freeze-trapped assays

Schema {🗺️}

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         headline:Stable isotope resolved metabolomics of lung cancer in a SCID mouse model
         description:We have determined the time course of [U-13C]-glucose utilization and transformations in SCID mice via bolus injection of the tracer in the tail vein. Incorporation of 13C into metabolites extracted from mouse blood plasma and several tissues (lung, heart, brain, liver, kidney, and skeletal muscle) were profiled by NMR and GC–MS, which helped ascertain optimal sampling times for different target tissues. We found that the time for overall optimal 13C incorporation into tissue was 15–20 min but with substantial differences in 13C labeling patterns of various organs that reflected their specific metabolism. Using this stable isotope resolved metabolomics (SIRM) approach, we have compared the 13C metabolite profile of the lungs in the same mouse with or without an orthotopic lung tumor xenograft established from human PC14PE6 lung adenocarcinoma cells. The 13C metabolite profile shows considerable differences in [U-13C]-glucose transformations between the two lung tissues, demonstrating the feasibility of applying SIRM to investigate metabolic networks of human cancer xenograft in the mouse model.
         datePublished:2010-10-28T00:00:00Z
         dateModified:2010-10-28T00:00:00Z
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            SCID mouse
            Metabolomics
            Non-small cell lung cancer xenograft
            Biochemistry
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            Molecular Medicine
            Cell Biology
            Developmental Biology
            Biomedicine
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      headline:Stable isotope resolved metabolomics of lung cancer in a SCID mouse model
      description:We have determined the time course of [U-13C]-glucose utilization and transformations in SCID mice via bolus injection of the tracer in the tail vein. Incorporation of 13C into metabolites extracted from mouse blood plasma and several tissues (lung, heart, brain, liver, kidney, and skeletal muscle) were profiled by NMR and GC–MS, which helped ascertain optimal sampling times for different target tissues. We found that the time for overall optimal 13C incorporation into tissue was 15–20 min but with substantial differences in 13C labeling patterns of various organs that reflected their specific metabolism. Using this stable isotope resolved metabolomics (SIRM) approach, we have compared the 13C metabolite profile of the lungs in the same mouse with or without an orthotopic lung tumor xenograft established from human PC14PE6 lung adenocarcinoma cells. The 13C metabolite profile shows considerable differences in [U-13C]-glucose transformations between the two lung tissues, demonstrating the feasibility of applying SIRM to investigate metabolic networks of human cancer xenograft in the mouse model.
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         Metabolomics
         Non-small cell lung cancer xenograft
         Biochemistry
         general
         Molecular Medicine
         Cell Biology
         Developmental Biology
         Biomedicine
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