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Keywords {🔍}

tmb, conversion, mouse, cre, allele, pubmed, htncre, article, embryos, google, scholar, cell, mice, cas, tma, cmvcre, ivf, breeding, gene, lacz, excision, driver, sperm, expression, line, treatment, mutant, central, full, neo, females, table, phenotyping, strains, cassette, eucommkompcsd, recombinase, sanger, time, fig, reporter, transgenic, project, knockout, size, supplementary, showed, information, research, highthroughput,

Topics {✒️}

ccr2/ccr5 double-deficient mice tyh media + methyl-β-cyclodextrin eucomm/komp-csd strain prmt5tm2a ramiro ramirez-solis eucomm/komp-csd mouse strains x-linked cmv-cre driver pre-incubation tyh + mbcd drop eucomm/komp-csd tm1a allele article download pdf c57bl/6n genetic background cell-permeable cre recombinase eucomm/komp-csd alleles wild type c57bl/6n eucomm/komp-csd collection tat-nls-tagged cre cell permeable htn-cre x-linked cre driver modified cryo-protective agent cre-transgenic mouse strain c57bl/6n wt mice mating super-ovulated females htn-cre technique works cmv-cre transgenic breeding cmv-cre homozygous females high-resolution expression analysis viable long-term tool methyl-beta-cyclodextrin cmv-cre transgenic animals cmv-cre breeding method mouse genetics project htn-cre treated gt htn-cre protein method end-point assays amplify sperm pre-incubation dishes high-throughput mouse production 3 μm htn-cre increased dmem + htn-cre solution lower cage/week expenses htn-cre treatment takes c57bl/6ntac females subsequent htn-cre treatment unexpected embryonic lethality htn-cre mediated conversion cmv-cre mating set 3 μm htn-cre treatment qpcr-based assays detect cell-permeable cre cell permeable cre create recombination events table 3 htn-cre treatment

Schema {🗺️}

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         headline:Rapid conversion of EUCOMM/KOMP-CSD alleles in mouse embryos using a cell-permeable Cre recombinase
         description:We describe here use of a cell-permeable Cre to efficiently convert the EUCOMM/KOMP-CSD tm1a allele to the tm1b form in preimplantation mouse embryos in a high-throughput manner, consistent with the requirements of the International Mouse Phenotyping Consortium-affiliated NIH KOMP2 project. This method results in rapid allele conversion and minimizes the use of experimental animals when compared to conventional Cre transgenic mouse breeding, resulting in a significant reduction in costs and time with increased welfare benefits.
         datePublished:2013-11-07T00:00:00Z
         dateModified:2013-11-07T00:00:00Z
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            Mammalian
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            Biomedical Engineering/Biotechnology
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      headline:Rapid conversion of EUCOMM/KOMP-CSD alleles in mouse embryos using a cell-permeable Cre recombinase
      description:We describe here use of a cell-permeable Cre to efficiently convert the EUCOMM/KOMP-CSD tm1a allele to the tm1b form in preimplantation mouse embryos in a high-throughput manner, consistent with the requirements of the International Mouse Phenotyping Consortium-affiliated NIH KOMP2 project. This method results in rapid allele conversion and minimizes the use of experimental animals when compared to conventional Cre transgenic mouse breeding, resulting in a significant reduction in costs and time with increased welfare benefits.
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      name:The Wellcome Trust Sanger Institute, Cambridgeshire, UK
      name:The Wellcome Trust Sanger Institute, Cambridgeshire, UK
      name:The Wellcome Trust Sanger Institute, Cambridgeshire, UK
      name:The Wellcome Trust Sanger Institute, Cambridgeshire, UK
      name:The Wellcome Trust Sanger Institute, Cambridgeshire, UK
      name:The Wellcome Trust Sanger Institute, Cambridgeshire, UK
      name:The Wellcome Trust Sanger Institute, Cambridgeshire, UK
      name:The Wellcome Trust Sanger Institute, Cambridgeshire, UK
      name:The Wellcome Trust Sanger Institute, Cambridgeshire, UK
      name:The Wellcome Trust Sanger Institute, Cambridgeshire, UK
      name:The Wellcome Trust Sanger Institute, Cambridgeshire, UK
      name:The Wellcome Trust Sanger Institute, Cambridgeshire, UK
      name:The Wellcome Trust Sanger Institute, Cambridgeshire, UK
      name:The Wellcome Trust Sanger Institute, Cambridgeshire, UK
      name:The Wellcome Trust Sanger Institute, Cambridgeshire, UK
      name:The Wellcome Trust Sanger Institute, Cambridgeshire, UK
      name:The Wellcome Trust Sanger Institute, Cambridgeshire, UK

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