We are analyzing https://link.springer.com/article/10.1007/s11248-012-9635-x.

Title:
Production of mouse pups from germline transmission-failed knockout chimeras | Transgenic Research
Description:
Occasionally, chimeras do not transmit the gene of interest to pups in gene disruption experiments. However, the risk of failure could be reduced if we could identify embryonic stem (ES)-derived germ cells in the testis. Here, we report the production of pups from three lines of infertile chimeric male mice and the establishment of knockout lines by combining green fluorescent protein-tagged ES cells with intracytoplasmic sperm injection.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Education
Science
Technology & Computing

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
However, some sources were not loaded, we suggest to reload the page to get complete results.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We don’t know how the website earns money.

Not every website is profit-driven; some are created to spread information or serve as an online presence. Websites can be made for many reasons. This could be one of them. Link.springer.com might be cashing in, but we can't detect the method they're using.

Keywords {🔍}

article, google, scholar, cells, mouse, pubmed, cas, okabe, ikawa, research, sperm, privacy, cookies, germ, content, publish, search, transgenic, pups, knockout, fujihara, kaseda, inoue, gene, embryonic, stem, mice, access, nature, data, information, log, journal, production, germline, chimeras, july, masaru, green, discover, development, dev, nishimune, nakanishi, download, author, supplementary, material, pdf, springer,

Topics {✒️}

embryonic stem cells identify embryonic stem male germ cells derived germ cells month download article/chapter ubiquitous green cells masahito ikawa & masaru okabe privacy choices/manage cookies full article pdf conditional knockout resource european economic area gp130-mediated signaling putative chaperone calmegin real-time observation de jong pj genome-wide study article fujihara conditions privacy policy intracytoplasmic sperm injection fully fertile sperm �green mice technical support preimplantation development electronic supplementary material mouse oocytes injected accepting optional cookies gene disruption experiments transgenic res 22 main content log author information authors chimeric mice journal finder publish check access instant access masaru okabe article log article cite development 121 marker protein mouse gene function es knockout lines related subjects privacy policy personal data laboratory manual books a supplementary material 1 optional cookies manage preferences

Schema {🗺️}

WebPage:
      mainEntity:
         headline:Production of mouse pups from germline transmission-failed knockout chimeras
         description:Occasionally, chimeras do not transmit the gene of interest to pups in gene disruption experiments. However, the risk of failure could be reduced if we could identify embryonic stem (ES)-derived germ cells in the testis. Here, we report the production of pups from three lines of infertile chimeric male mice and the establishment of knockout lines by combining green fluorescent protein-tagged ES cells with intracytoplasmic sperm injection.
         datePublished:2012-07-24T00:00:00Z
         dateModified:2012-07-24T00:00:00Z
         pageStart:195
         pageEnd:200
         sameAs:https://doi.org/10.1007/s11248-012-9635-x
         keywords:
            ES cells
            C57BL/6N
            Transgenic mice
            EGFP
            ICSI
            Animal Genetics and Genomics
            Plant Genetics and Genomics
            Transgenics
            Biomedical Engineering/Biotechnology
            Genetic Engineering
            Molecular Medicine
         image:
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11248-012-9635-x/MediaObjects/11248_2012_9635_Fig1_HTML.jpg
         isPartOf:
            name:Transgenic Research
            issn:
               1573-9368
               0962-8819
            volumeNumber:22
            type:
               Periodical
               PublicationVolume
         publisher:
            name:Springer Netherlands
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Yoshitaka Fujihara
               affiliation:
                     name:Osaka University
                     address:
                        name:Research Institute for Microbial Diseases, Osaka University, Suita, Japan
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Kazuhiro Kaseda
               affiliation:
                     name:Osaka University
                     address:
                        name:Research Institute for Microbial Diseases, Osaka University, Suita, Japan
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Naokazu Inoue
               affiliation:
                     name:Osaka University
                     address:
                        name:Research Institute for Microbial Diseases, Osaka University, Suita, Japan
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Masahito Ikawa
               affiliation:
                     name:Osaka University
                     address:
                        name:Research Institute for Microbial Diseases, Osaka University, Suita, Japan
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Masaru Okabe
               affiliation:
                     name:Osaka University
                     address:
                        name:Research Institute for Microbial Diseases, Osaka University, Suita, Japan
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
         isAccessibleForFree:
         hasPart:
            isAccessibleForFree:
            cssSelector:.main-content
            type:WebPageElement
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:Production of mouse pups from germline transmission-failed knockout chimeras
      description:Occasionally, chimeras do not transmit the gene of interest to pups in gene disruption experiments. However, the risk of failure could be reduced if we could identify embryonic stem (ES)-derived germ cells in the testis. Here, we report the production of pups from three lines of infertile chimeric male mice and the establishment of knockout lines by combining green fluorescent protein-tagged ES cells with intracytoplasmic sperm injection.
      datePublished:2012-07-24T00:00:00Z
      dateModified:2012-07-24T00:00:00Z
      pageStart:195
      pageEnd:200
      sameAs:https://doi.org/10.1007/s11248-012-9635-x
      keywords:
         ES cells
         C57BL/6N
         Transgenic mice
         EGFP
         ICSI
         Animal Genetics and Genomics
         Plant Genetics and Genomics
         Transgenics
         Biomedical Engineering/Biotechnology
         Genetic Engineering
         Molecular Medicine
      image:
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11248-012-9635-x/MediaObjects/11248_2012_9635_Fig1_HTML.jpg
      isPartOf:
         name:Transgenic Research
         issn:
            1573-9368
            0962-8819
         volumeNumber:22
         type:
            Periodical
            PublicationVolume
      publisher:
         name:Springer Netherlands
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Yoshitaka Fujihara
            affiliation:
                  name:Osaka University
                  address:
                     name:Research Institute for Microbial Diseases, Osaka University, Suita, Japan
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Kazuhiro Kaseda
            affiliation:
                  name:Osaka University
                  address:
                     name:Research Institute for Microbial Diseases, Osaka University, Suita, Japan
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Naokazu Inoue
            affiliation:
                  name:Osaka University
                  address:
                     name:Research Institute for Microbial Diseases, Osaka University, Suita, Japan
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Masahito Ikawa
            affiliation:
                  name:Osaka University
                  address:
                     name:Research Institute for Microbial Diseases, Osaka University, Suita, Japan
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Masaru Okabe
            affiliation:
                  name:Osaka University
                  address:
                     name:Research Institute for Microbial Diseases, Osaka University, Suita, Japan
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
["Periodical","PublicationVolume"]:
      name:Transgenic Research
      issn:
         1573-9368
         0962-8819
      volumeNumber:22
Organization:
      name:Springer Netherlands
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Osaka University
      address:
         name:Research Institute for Microbial Diseases, Osaka University, Suita, Japan
         type:PostalAddress
      name:Osaka University
      address:
         name:Research Institute for Microbial Diseases, Osaka University, Suita, Japan
         type:PostalAddress
      name:Osaka University
      address:
         name:Research Institute for Microbial Diseases, Osaka University, Suita, Japan
         type:PostalAddress
      name:Osaka University
      address:
         name:Research Institute for Microbial Diseases, Osaka University, Suita, Japan
         type:PostalAddress
      name:Osaka University
      address:
         name:Research Institute for Microbial Diseases, Osaka University, Suita, Japan
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Yoshitaka Fujihara
      affiliation:
            name:Osaka University
            address:
               name:Research Institute for Microbial Diseases, Osaka University, Suita, Japan
               type:PostalAddress
            type:Organization
      name:Kazuhiro Kaseda
      affiliation:
            name:Osaka University
            address:
               name:Research Institute for Microbial Diseases, Osaka University, Suita, Japan
               type:PostalAddress
            type:Organization
      name:Naokazu Inoue
      affiliation:
            name:Osaka University
            address:
               name:Research Institute for Microbial Diseases, Osaka University, Suita, Japan
               type:PostalAddress
            type:Organization
      name:Masahito Ikawa
      affiliation:
            name:Osaka University
            address:
               name:Research Institute for Microbial Diseases, Osaka University, Suita, Japan
               type:PostalAddress
            type:Organization
      name:Masaru Okabe
      affiliation:
            name:Osaka University
            address:
               name:Research Institute for Microbial Diseases, Osaka University, Suita, Japan
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Research Institute for Microbial Diseases, Osaka University, Suita, Japan
      name:Research Institute for Microbial Diseases, Osaka University, Suita, Japan
      name:Research Institute for Microbial Diseases, Osaka University, Suita, Japan
      name:Research Institute for Microbial Diseases, Osaka University, Suita, Japan
      name:Research Institute for Microbial Diseases, Osaka University, Suita, Japan
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

External Links {🔗}(76)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

CDN Services {📦}

Crossref

5.01s.

LINK . SPRINGER . COM {}