We are analyzing https://link.springer.com/article/10.1007/s11095-005-9043-5.

Title:
Smad3 Specific Inhibitor, Naringenin, Decreases the Expression of Extracellular Matrix Induced by TGF-β1 in Cultured Rat Hepatic Stellate Cells | Pharmaceutical Research
Description:
Purpose During the process of liver fibrogenesis, transforming growth factor-β (TGF-β) plays an essential role in modulating extracellular matrix (ECM) gene expression, and a growing body of evidence suggests that this is a Smad3-dependent process in the activated hepatic stellate cells (HSCs). Naringenin showed a significantly protective effect on experimental rat liver fibrosis, in our efforts to elucidate its antifibrosis molecular mechanisms and to find a novel target based on Smad3 signaling for challenging fibrosis diseases. Methods In this study, reverse transcription-polymerase chain reaction and Western blot assays were used to investigate the inhibitory effect of naringenin on ECM formation induced by TGF-β1 in the HSC-T6 cells. Results Naringenin reduced not only the accumulation of ECM, including collagen Iα1 (Col Iα1), fibronectin (FN), and plasminogen activator inhibitor-1 (PAI-1), but also the production of Smad3 induced by TGF-β1 in both mRNA and protein levels in a dose-dependent manner. Moreover, naringenin selectively inhibited the transcription of Smad3, but not other Smads involved in TGF-β1 signaling pathways. Conclusion Our data demonstrate that naringenin can exert antifibrogenic effects by directly or indirectly down-regulating Smad3 protein expression and phosphorylation through TGF-β signaling.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Education
Science
Social Networks

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
However, some sources were not loaded, we suggest to reload the page to get complete results.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We can't figure out the monetization strategy.

Some websites aren't about earning revenue; they're built to connect communities or raise awareness. There are numerous motivations behind creating websites. This might be one of them. Link.springer.com might be cashing in, but we can't detect the method they're using.

Keywords {🔍}

google, scholar, cas, article, pubmed, liver, smad, hepatic, cells, growth, fibrosis, naringenin, stellate, transforming, factor, activation, expression, tgfβ, beta, matrix, signaling, access, cell, research, extracellular, wang, fibrogenesis, tgfbeta, receptor, inhibition, privacy, cookies, content, inhibitor, wei, role, gene, plasminogen, activator, rats, biol, data, publish, search, induced, rat, liu, ecm, effect, collagen,

Topics {✒️}

month download article/chapter transforming growth factor-beta tgf-beta/smad gene targets phosphatidylinositol 3-kinase/akt-dependent suppression tgf-beta soluble receptor tgf-β1/smad/snon pathway transforming growth factor-β tgf-beta signaling pathways hepatic stellate cells related subjects tgf-β1 signaling pathways smad3/4-dependent transcriptional activation smad3 specific inhibitor extracellular matrix induced iglesias-de la cruz hepatic fibrosis proc tgf-β1/smad2/3 signaling full article pdf modulating extracellular matrix smad7 prevents activation plasminogen activator inhibitor-1 plasminogen activator inhibitor tgf-beta signaling mesangial cells biochem citrus flavonoid naringenin insulin involves activation hsc-t6 cells fat-storing cells privacy choices/manage cookies stellate cells noncollagenous matrix proteins inhibits transcriptional responses challenging fibrosis diseases type 1 receptor growth factor including collagen iα1 diabetic mouse kidney article liu transgenic mice results shenyang pharmaceutical university hepatic fibrosis tgf-β signaling check access instant access col iα1 %2bb2cnhvfw%3d 2186487 10 dose-dependent manner liver fibrosis liver fibrosis article log

Schema {🗺️}

WebPage:
      mainEntity:
         headline:Smad3 Specific Inhibitor, Naringenin, Decreases the Expression of Extracellular Matrix Induced by TGF-β1 in Cultured Rat Hepatic Stellate Cells
         description:During the process of liver fibrogenesis, transforming growth factor-β (TGF-β) plays an essential role in modulating extracellular matrix (ECM) gene expression, and a growing body of evidence suggests that this is a Smad3-dependent process in the activated hepatic stellate cells (HSCs). Naringenin showed a significantly protective effect on experimental rat liver fibrosis, in our efforts to elucidate its antifibrosis molecular mechanisms and to find a novel target based on Smad3 signaling for challenging fibrosis diseases. In this study, reverse transcription-polymerase chain reaction and Western blot assays were used to investigate the inhibitory effect of naringenin on ECM formation induced by TGF-β1 in the HSC-T6 cells. Naringenin reduced not only the accumulation of ECM, including collagen Iα1 (Col Iα1), fibronectin (FN), and plasminogen activator inhibitor-1 (PAI-1), but also the production of Smad3 induced by TGF-β1 in both mRNA and protein levels in a dose-dependent manner. Moreover, naringenin selectively inhibited the transcription of Smad3, but not other Smads involved in TGF-β1 signaling pathways. Our data demonstrate that naringenin can exert antifibrogenic effects by directly or indirectly down-regulating Smad3 protein expression and phosphorylation through TGF-β signaling.
         datePublished:2006-12-14T00:00:00Z
         dateModified:2006-12-14T00:00:00Z
         pageStart:82
         pageEnd:89
         sameAs:https://doi.org/10.1007/s11095-005-9043-5
         keywords:
            extracellular matrix
            liver fibrosis
            naringenin
            Smad3
            transforming growth factor-1
            Pharmacology/Toxicology
            Pharmacy
            Biochemistry
            general
            Medical Law
            Biomedical Engineering and Bioengineering
         image:
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11095-005-9043-5/MediaObjects/NY00009043_FIG_01.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11095-005-9043-5/MediaObjects/NY00009043_FIG_02.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11095-005-9043-5/MediaObjects/NY00009043_FIG_03.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11095-005-9043-5/MediaObjects/NY00009043_FIG_04.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11095-005-9043-5/MediaObjects/NY00009043_FIG_05.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11095-005-9043-5/MediaObjects/NY00009043_FIG_06.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11095-005-9043-5/MediaObjects/NY00009043_FIG_07.gif
         isPartOf:
            name:Pharmaceutical Research
            issn:
               1573-904X
               0724-8741
            volumeNumber:23
            type:
               Periodical
               PublicationVolume
         publisher:
            name:Springer-Verlag
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Xingjun Liu
               affiliation:
                     name:Chinese Academy of Sciences
                     address:
                        name:National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
                        type:PostalAddress
                     type:Organization
                     name:Shenyang Pharmaceutical University
                     address:
                        name:School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Wei Wang
               affiliation:
                     name:Chinese Academy of Sciences
                     address:
                        name:National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Han Hu
               affiliation:
                     name:Chinese Academy of Sciences
                     address:
                        name:National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Ning Tang
               affiliation:
                     name:Chinese Academy of Sciences
                     address:
                        name:National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Chunling Zhang
               affiliation:
                     name:Chinese Academy of Sciences
                     address:
                        name:National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Wei Liang
               affiliation:
                     name:Chinese Academy of Sciences
                     address:
                        name:National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
               name:Minwei Wang
               affiliation:
                     name:Shenyang Pharmaceutical University
                     address:
                        name:School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China
                        type:PostalAddress
                     type:Organization
               type:Person
         isAccessibleForFree:
         hasPart:
            isAccessibleForFree:
            cssSelector:.main-content
            type:WebPageElement
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:Smad3 Specific Inhibitor, Naringenin, Decreases the Expression of Extracellular Matrix Induced by TGF-β1 in Cultured Rat Hepatic Stellate Cells
      description:During the process of liver fibrogenesis, transforming growth factor-β (TGF-β) plays an essential role in modulating extracellular matrix (ECM) gene expression, and a growing body of evidence suggests that this is a Smad3-dependent process in the activated hepatic stellate cells (HSCs). Naringenin showed a significantly protective effect on experimental rat liver fibrosis, in our efforts to elucidate its antifibrosis molecular mechanisms and to find a novel target based on Smad3 signaling for challenging fibrosis diseases. In this study, reverse transcription-polymerase chain reaction and Western blot assays were used to investigate the inhibitory effect of naringenin on ECM formation induced by TGF-β1 in the HSC-T6 cells. Naringenin reduced not only the accumulation of ECM, including collagen Iα1 (Col Iα1), fibronectin (FN), and plasminogen activator inhibitor-1 (PAI-1), but also the production of Smad3 induced by TGF-β1 in both mRNA and protein levels in a dose-dependent manner. Moreover, naringenin selectively inhibited the transcription of Smad3, but not other Smads involved in TGF-β1 signaling pathways. Our data demonstrate that naringenin can exert antifibrogenic effects by directly or indirectly down-regulating Smad3 protein expression and phosphorylation through TGF-β signaling.
      datePublished:2006-12-14T00:00:00Z
      dateModified:2006-12-14T00:00:00Z
      pageStart:82
      pageEnd:89
      sameAs:https://doi.org/10.1007/s11095-005-9043-5
      keywords:
         extracellular matrix
         liver fibrosis
         naringenin
         Smad3
         transforming growth factor-1
         Pharmacology/Toxicology
         Pharmacy
         Biochemistry
         general
         Medical Law
         Biomedical Engineering and Bioengineering
      image:
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11095-005-9043-5/MediaObjects/NY00009043_FIG_01.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11095-005-9043-5/MediaObjects/NY00009043_FIG_02.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11095-005-9043-5/MediaObjects/NY00009043_FIG_03.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11095-005-9043-5/MediaObjects/NY00009043_FIG_04.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11095-005-9043-5/MediaObjects/NY00009043_FIG_05.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11095-005-9043-5/MediaObjects/NY00009043_FIG_06.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11095-005-9043-5/MediaObjects/NY00009043_FIG_07.gif
      isPartOf:
         name:Pharmaceutical Research
         issn:
            1573-904X
            0724-8741
         volumeNumber:23
         type:
            Periodical
            PublicationVolume
      publisher:
         name:Springer-Verlag
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Xingjun Liu
            affiliation:
                  name:Chinese Academy of Sciences
                  address:
                     name:National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
                     type:PostalAddress
                  type:Organization
                  name:Shenyang Pharmaceutical University
                  address:
                     name:School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Wei Wang
            affiliation:
                  name:Chinese Academy of Sciences
                  address:
                     name:National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Han Hu
            affiliation:
                  name:Chinese Academy of Sciences
                  address:
                     name:National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Ning Tang
            affiliation:
                  name:Chinese Academy of Sciences
                  address:
                     name:National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Chunling Zhang
            affiliation:
                  name:Chinese Academy of Sciences
                  address:
                     name:National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Wei Liang
            affiliation:
                  name:Chinese Academy of Sciences
                  address:
                     name:National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
            name:Minwei Wang
            affiliation:
                  name:Shenyang Pharmaceutical University
                  address:
                     name:School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China
                     type:PostalAddress
                  type:Organization
            type:Person
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
["Periodical","PublicationVolume"]:
      name:Pharmaceutical Research
      issn:
         1573-904X
         0724-8741
      volumeNumber:23
Organization:
      name:Springer-Verlag
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Chinese Academy of Sciences
      address:
         name:National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
         type:PostalAddress
      name:Shenyang Pharmaceutical University
      address:
         name:School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China
         type:PostalAddress
      name:Chinese Academy of Sciences
      address:
         name:National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
         type:PostalAddress
      name:Chinese Academy of Sciences
      address:
         name:National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
         type:PostalAddress
      name:Chinese Academy of Sciences
      address:
         name:National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
         type:PostalAddress
      name:Chinese Academy of Sciences
      address:
         name:National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
         type:PostalAddress
      name:Chinese Academy of Sciences
      address:
         name:National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
         type:PostalAddress
      name:Shenyang Pharmaceutical University
      address:
         name:School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Xingjun Liu
      affiliation:
            name:Chinese Academy of Sciences
            address:
               name:National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
               type:PostalAddress
            type:Organization
            name:Shenyang Pharmaceutical University
            address:
               name:School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China
               type:PostalAddress
            type:Organization
      name:Wei Wang
      affiliation:
            name:Chinese Academy of Sciences
            address:
               name:National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
               type:PostalAddress
            type:Organization
      name:Han Hu
      affiliation:
            name:Chinese Academy of Sciences
            address:
               name:National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
               type:PostalAddress
            type:Organization
      name:Ning Tang
      affiliation:
            name:Chinese Academy of Sciences
            address:
               name:National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
               type:PostalAddress
            type:Organization
      name:Chunling Zhang
      affiliation:
            name:Chinese Academy of Sciences
            address:
               name:National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
               type:PostalAddress
            type:Organization
      name:Wei Liang
      affiliation:
            name:Chinese Academy of Sciences
            address:
               name:National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
               type:PostalAddress
            type:Organization
      email:[email protected]
      name:Minwei Wang
      affiliation:
            name:Shenyang Pharmaceutical University
            address:
               name:School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China
               type:PostalAddress
            type:Organization
PostalAddress:
      name:National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
      name:School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China
      name:National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
      name:National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
      name:National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
      name:National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
      name:National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
      name:School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

External Links {🔗}(122)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

CDN Services {📦}

Crossref

3.93s.

LINK . SPRINGER . COM {}