We are analyzing https://link.springer.com/article/10.1007/s11064-005-6771-1.

Title:
A Study of Glutathione S-transferase pi Expression in Central Nervous System of Subjects with Amyotrophic Lateral Sclerosis Using RNA Extraction from Formalin-Fixed, Paraffin-Embedded Material | Neurochemical Research
Description:
The expression of glutathione S-transferase pi (GST pi), an enzyme responsible for inactivation of a large variety of toxic compounds was studied in spinal cord, motor and sensory brain cortex obtained from patients who died in the course of amyotrophic lateral sclerosis (ALS). The studies were performed on formalin-fixed, paraffin-embedded (FFPE) and freshly frozen tissues. The method of RNA isolation from FFPE was modified. A significant decrease of GST pi-mRNA expression was found in cervical spinal cord and motor brain cortex of ALS subjects comparing to analogue control tissues (P < 0.01), as well as in motor cortex of ALS subjects comparing to their sensory cortex (P < 0.05). In spinal cords the decrease in GST pi-mRNA expression was accompanied by a decrease of GST pi protein level. Results indicated lowered GST pi expression on both mRNA and protein levels in the regions of nervous system affected by ALS. The non-properly inactivated by GST toxic electrophiles and organic peroxides may thus contribute to motor neurons damage.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Education
Science
Mobile Technology & AI

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
However, some sources were not loaded, we suggest to reload the page to get complete results.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We don’t know how the website earns money.

Not every website is profit-driven; some are created to spread information or serve as an online presence. Websites can be made for many reasons. This could be one of them. Link.springer.com could have a money-making trick up its sleeve, but it's undetectable for now.

Keywords {🔍}

google, scholar, article, pubmed, glutathione, expression, amyotrophic, lateral, sclerosis, brain, human, stransferase, formalinfixed, paraffinembedded, motor, gene, biochem, analysis, subjects, rna, gst, res, privacy, cookies, content, research, barańczykkuźma, spinal, cortex, access, publish, search, system, beata, kaźmierczak, kuźma, cord, stransferases, mannervik, warsaw, function, data, information, log, journal, nervous, material, usarek, gajewska, anna,

Topics {✒️}

brain glutathione-s-transferase neurochem paraffin-embedded material published amyotrophic lateral sclerosis paraffin-embedded material glutathione s-transferase pi cervical spinal cord paraffin-embedded tumor tissue glutathione-s-transferases pi month download article/chapter microdissected archival formalin-fixed spinal cord motor brain cortex human glutathione s-transferases gst pi-mrna expression glutathione s-transferase formalin-fixed samples methylmercury-exposed experimental neurotoxicity ifn-gamma mrna levels glutathione s-transferases anna barańczyk-kuźma glutathione transferase catalyse astrocytoma brain biochim mdr1 gene expression peripheral motor nerve paraffin-embedded privacy choices/manage cookies full article pdf motor cortex motor neurons damage antioxidant drugs block mdr1 mrna expression human brain central nervous system freshly frozen tissues analogue control tissues spinal cords nervous system affected oxidative stress biochem check access instant access formalin-fixed ppar-gamma signaling european economic area sensory cortex gst toxic electrophiles immunologically distinct types molecular biology application 26500-mr subunits biochem conditions privacy policy related subjects

Schema {🗺️}

WebPage:
      mainEntity:
         headline:A Study of Glutathione S-transferase pi Expression in Central Nervous System of Subjects with Amyotrophic Lateral Sclerosis Using RNA Extraction from Formalin-Fixed, Paraffin-Embedded Material
         description:The expression of glutathione S-transferase pi (GST pi), an enzyme responsible for inactivation of a large variety of toxic compounds was studied in spinal cord, motor and sensory brain cortex obtained from patients who died in the course of amyotrophic lateral sclerosis (ALS). The studies were performed on formalin-fixed, paraffin-embedded (FFPE) and freshly frozen tissues. The method of RNA isolation from FFPE was modified. A significant decrease of GST pi-mRNA expression was found in cervical spinal cord and motor brain cortex of ALS subjects comparing to analogue control tissues (P < 0.01), as well as in motor cortex of ALS subjects comparing to their sensory cortex (P < 0.05). In spinal cords the decrease in GST pi-mRNA expression was accompanied by a decrease of GST pi protein level. Results indicated lowered GST pi expression on both mRNA and protein levels in the regions of nervous system affected by ALS. The non-properly inactivated by GST toxic electrophiles and organic peroxides may thus contribute to motor neurons damage.
         datePublished:
         dateModified:
         pageStart:1003
         pageEnd:1007
         sameAs:https://doi.org/10.1007/s11064-005-6771-1
         keywords:
            Amyotrophic lateral sclerosis
            brain cortex
            formalin-fixed
            Glutathione S-transferase pi
            paraffin-embedded material
            spinal cord
            Neurosciences
            Neurochemistry
            Biochemistry
            general
            Cell Biology
            Neurology
         image:
         isPartOf:
            name:Neurochemical Research
            issn:
               1573-6903
               0364-3190
            volumeNumber:30
            type:
               Periodical
               PublicationVolume
         publisher:
            name:Kluwer Academic Publishers-Plenum Publishers
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Ewa Usarek
               affiliation:
                     name:Medical University of Warsaw
                     address:
                        name:Chair and Department of Biochemistry, Medical University of Warsaw, Warsaw, Poland
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Beata Gajewska
               affiliation:
                     name:Medical University of Warsaw
                     address:
                        name:Chair and Department of Biochemistry, Medical University of Warsaw, Warsaw, Poland
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Beata Kaźmierczak
               affiliation:
                     name:Medical University of Warsaw
                     address:
                        name:Chair and Department of Biochemistry, Medical University of Warsaw, Warsaw, Poland
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Magdalena Kuźma
               affiliation:
                     name:Medical University of Warsaw
                     address:
                        name:Department of Neurology, Medical University of Warsaw, Warsaw, Poland
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Dorota Dziewulska
               affiliation:
                     name:Medical University of Warsaw
                     address:
                        name:Department of Neurology, Medical University of Warsaw, Warsaw, Poland
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Anna Barańczyk-Kuźma
               affiliation:
                     name:Medical University of Warsaw
                     address:
                        name:Chair and Department of Biochemistry, Medical University of Warsaw, Warsaw, Poland
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
         isAccessibleForFree:
         hasPart:
            isAccessibleForFree:
            cssSelector:.main-content
            type:WebPageElement
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:A Study of Glutathione S-transferase pi Expression in Central Nervous System of Subjects with Amyotrophic Lateral Sclerosis Using RNA Extraction from Formalin-Fixed, Paraffin-Embedded Material
      description:The expression of glutathione S-transferase pi (GST pi), an enzyme responsible for inactivation of a large variety of toxic compounds was studied in spinal cord, motor and sensory brain cortex obtained from patients who died in the course of amyotrophic lateral sclerosis (ALS). The studies were performed on formalin-fixed, paraffin-embedded (FFPE) and freshly frozen tissues. The method of RNA isolation from FFPE was modified. A significant decrease of GST pi-mRNA expression was found in cervical spinal cord and motor brain cortex of ALS subjects comparing to analogue control tissues (P < 0.01), as well as in motor cortex of ALS subjects comparing to their sensory cortex (P < 0.05). In spinal cords the decrease in GST pi-mRNA expression was accompanied by a decrease of GST pi protein level. Results indicated lowered GST pi expression on both mRNA and protein levels in the regions of nervous system affected by ALS. The non-properly inactivated by GST toxic electrophiles and organic peroxides may thus contribute to motor neurons damage.
      datePublished:
      dateModified:
      pageStart:1003
      pageEnd:1007
      sameAs:https://doi.org/10.1007/s11064-005-6771-1
      keywords:
         Amyotrophic lateral sclerosis
         brain cortex
         formalin-fixed
         Glutathione S-transferase pi
         paraffin-embedded material
         spinal cord
         Neurosciences
         Neurochemistry
         Biochemistry
         general
         Cell Biology
         Neurology
      image:
      isPartOf:
         name:Neurochemical Research
         issn:
            1573-6903
            0364-3190
         volumeNumber:30
         type:
            Periodical
            PublicationVolume
      publisher:
         name:Kluwer Academic Publishers-Plenum Publishers
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Ewa Usarek
            affiliation:
                  name:Medical University of Warsaw
                  address:
                     name:Chair and Department of Biochemistry, Medical University of Warsaw, Warsaw, Poland
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Beata Gajewska
            affiliation:
                  name:Medical University of Warsaw
                  address:
                     name:Chair and Department of Biochemistry, Medical University of Warsaw, Warsaw, Poland
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Beata Kaźmierczak
            affiliation:
                  name:Medical University of Warsaw
                  address:
                     name:Chair and Department of Biochemistry, Medical University of Warsaw, Warsaw, Poland
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Magdalena Kuźma
            affiliation:
                  name:Medical University of Warsaw
                  address:
                     name:Department of Neurology, Medical University of Warsaw, Warsaw, Poland
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Dorota Dziewulska
            affiliation:
                  name:Medical University of Warsaw
                  address:
                     name:Department of Neurology, Medical University of Warsaw, Warsaw, Poland
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Anna Barańczyk-Kuźma
            affiliation:
                  name:Medical University of Warsaw
                  address:
                     name:Chair and Department of Biochemistry, Medical University of Warsaw, Warsaw, Poland
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
["Periodical","PublicationVolume"]:
      name:Neurochemical Research
      issn:
         1573-6903
         0364-3190
      volumeNumber:30
Organization:
      name:Kluwer Academic Publishers-Plenum Publishers
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Medical University of Warsaw
      address:
         name:Chair and Department of Biochemistry, Medical University of Warsaw, Warsaw, Poland
         type:PostalAddress
      name:Medical University of Warsaw
      address:
         name:Chair and Department of Biochemistry, Medical University of Warsaw, Warsaw, Poland
         type:PostalAddress
      name:Medical University of Warsaw
      address:
         name:Chair and Department of Biochemistry, Medical University of Warsaw, Warsaw, Poland
         type:PostalAddress
      name:Medical University of Warsaw
      address:
         name:Department of Neurology, Medical University of Warsaw, Warsaw, Poland
         type:PostalAddress
      name:Medical University of Warsaw
      address:
         name:Department of Neurology, Medical University of Warsaw, Warsaw, Poland
         type:PostalAddress
      name:Medical University of Warsaw
      address:
         name:Chair and Department of Biochemistry, Medical University of Warsaw, Warsaw, Poland
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Ewa Usarek
      affiliation:
            name:Medical University of Warsaw
            address:
               name:Chair and Department of Biochemistry, Medical University of Warsaw, Warsaw, Poland
               type:PostalAddress
            type:Organization
      name:Beata Gajewska
      affiliation:
            name:Medical University of Warsaw
            address:
               name:Chair and Department of Biochemistry, Medical University of Warsaw, Warsaw, Poland
               type:PostalAddress
            type:Organization
      name:Beata Kaźmierczak
      affiliation:
            name:Medical University of Warsaw
            address:
               name:Chair and Department of Biochemistry, Medical University of Warsaw, Warsaw, Poland
               type:PostalAddress
            type:Organization
      name:Magdalena Kuźma
      affiliation:
            name:Medical University of Warsaw
            address:
               name:Department of Neurology, Medical University of Warsaw, Warsaw, Poland
               type:PostalAddress
            type:Organization
      name:Dorota Dziewulska
      affiliation:
            name:Medical University of Warsaw
            address:
               name:Department of Neurology, Medical University of Warsaw, Warsaw, Poland
               type:PostalAddress
            type:Organization
      name:Anna Barańczyk-Kuźma
      affiliation:
            name:Medical University of Warsaw
            address:
               name:Chair and Department of Biochemistry, Medical University of Warsaw, Warsaw, Poland
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Chair and Department of Biochemistry, Medical University of Warsaw, Warsaw, Poland
      name:Chair and Department of Biochemistry, Medical University of Warsaw, Warsaw, Poland
      name:Chair and Department of Biochemistry, Medical University of Warsaw, Warsaw, Poland
      name:Department of Neurology, Medical University of Warsaw, Warsaw, Poland
      name:Department of Neurology, Medical University of Warsaw, Warsaw, Poland
      name:Chair and Department of Biochemistry, Medical University of Warsaw, Warsaw, Poland
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

External Links {🔗}(91)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

CDN Services {📦}

Crossref

4.12s.

LINK . SPRINGER . COM {}