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LINK . SPRINGER . COM {}

  1. Analyzed Page
  2. Matching Content Categories
  3. CMS
  4. Monthly Traffic Estimate
  5. How Does Link.springer.com Make Money
  6. Keywords
  7. Topics
  8. Questions
  9. Schema
  10. External Links
  11. Analytics And Tracking
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  13. CDN Services

We are analyzing https://link.springer.com/article/10.1007/s11060-013-1235-y.

Title:
Heparin blocks transfer of extracellular vesicles between donor and recipient cells | Journal of Neuro-Oncology
Description:
Extracellular vesicles (EVs) have been implicated in tumorigenesis. Biomolecules which can block EV binding and uptake into recipient cells may be of therapeutic value as well as enhance understanding of EV biology. Here, we show that heparin interacts with uptake of tumor-derived as well as non-tumor-derived EVs into recipient cells. Incubation of glioma cell-derived EVs with heparin resulted in micron-sized structures observed by transmission electron microscopy, with EVs clearly visible within these structures. Inclusion of heparin greatly diminished transfer of labeled EVs from donor to recipient tumor cells. We also show a direct interaction between heparin and EVs using confocal microscopy. We found that the block in EV uptake was at the level of cell binding and not internalization. Finally, incubation of glioma-derived EVs containing EGFRvIII mRNA with heparin reduced transfer of this message to recipient cells. The effect of heparin on EVs uptake may provide a unique tool to study EV function. It may also foster research of heparin or its derivatives as a therapeutic for disease in which EVs play a role.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

  • Education
  • Science
  • Non-Profit & Charity

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month


Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
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How Does Link.springer.com Make Money? {💸}

We can't see how the site brings in money.

Some websites aren't about earning revenue; they're built to connect communities or raise awareness. There are numerous motivations behind creating websites. This might be one of them. Link.springer.com has a revenue plan, but it's either invisible or we haven't found it.

Keywords {🔍}

article, google, scholar, pubmed, cas, heparin, supplementary, material, cancer, tiff, van, evs, cells, extracellular, tumor, cell, exosomes, usa, recipient, microscopy, access, center, privacy, cookies, content, journal, research, vesicles, balaj, maguire, human, thromb, xob, amsterdam, medical, publish, search, transfer, atai, noorden, uptake, clinical, growth, microvesicles, nat, biol, res, haemost, university, neuroscience,

Topics {✒️}

month download article/chapter syndecan-syntenin-alix regulates mgh/mit/hms athinoula dendritic cell-derived exosomes nih/nci grants ca069246 micron-sized structures observed anti-tumor progression effect glioma cell-derived evs human tumor-derived transmission electron microscopy related subjects van noorden cj henk van veen & cornelis engineered extracellular vesicles full article pdf harvard medical school promote tumor growth privacy choices/manage cookies heparin blocks transfer treating human malignancies tumor-derived evs recipient tumor cells neuro-oncology aims direct interaction �petites cellules” group academic medical center henk van veen van leeuwenhoek center nih grant p30ns045776 heparin reduced transfer glioma-derived evs gene delivery system image analysis core van sluis gl extracellular vesicles cbtrus statistical report distinct biologic roles amplified oncogene sequences thorley-lawson da de gruijl td hettiarachchi rj adjuvant intraportal infusion ornstein dl dos-santos ea de-paiva jf phs grant p30ns045776 massachusetts general hospital scientific advisory board conditions privacy policy article journal

Questions {❓}

  • Hettiarachchi RJ, Smorenburg SM, Ginsberg J, Levine M, Prins MH, Büller HR (1999) Do heparins do more than just treat thrombosis?
  • Nader HB, Chavante SF, dos-Santos EA, Oliveira TW, de-Paiva JF, Jerônimo SM et al (1999) Heparan sulfates and heparins: similar compounds performing the same functions in vertebrates and invertebrates?

Schema {🗺️}

WebPage:
      mainEntity:
         headline:Heparin blocks transfer of extracellular vesicles between donor and recipient cells
         description:Extracellular vesicles (EVs) have been implicated in tumorigenesis. Biomolecules which can block EV binding and uptake into recipient cells may be of therapeutic value as well as enhance understanding of EV biology. Here, we show that heparin interacts with uptake of tumor-derived as well as non-tumor-derived EVs into recipient cells. Incubation of glioma cell-derived EVs with heparin resulted in micron-sized structures observed by transmission electron microscopy, with EVs clearly visible within these structures. Inclusion of heparin greatly diminished transfer of labeled EVs from donor to recipient tumor cells. We also show a direct interaction between heparin and EVs using confocal microscopy. We found that the block in EV uptake was at the level of cell binding and not internalization. Finally, incubation of glioma-derived EVs containing EGFRvIII mRNA with heparin reduced transfer of this message to recipient cells. The effect of heparin on EVs uptake may provide a unique tool to study EV function. It may also foster research of heparin or its derivatives as a therapeutic for disease in which EVs play a role.
         datePublished:2013-09-04T00:00:00Z
         dateModified:2013-09-04T00:00:00Z
         pageStart:343
         pageEnd:351
         sameAs:https://doi.org/10.1007/s11060-013-1235-y
         keywords:
            Extracellular vesicles
            Exosomes
            Glioblastoma
            EGFRvIII
            Heparin
            Tumor
            Oncology
            Neurology
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ScholarlyArticle:
      headline:Heparin blocks transfer of extracellular vesicles between donor and recipient cells
      description:Extracellular vesicles (EVs) have been implicated in tumorigenesis. Biomolecules which can block EV binding and uptake into recipient cells may be of therapeutic value as well as enhance understanding of EV biology. Here, we show that heparin interacts with uptake of tumor-derived as well as non-tumor-derived EVs into recipient cells. Incubation of glioma cell-derived EVs with heparin resulted in micron-sized structures observed by transmission electron microscopy, with EVs clearly visible within these structures. Inclusion of heparin greatly diminished transfer of labeled EVs from donor to recipient tumor cells. We also show a direct interaction between heparin and EVs using confocal microscopy. We found that the block in EV uptake was at the level of cell binding and not internalization. Finally, incubation of glioma-derived EVs containing EGFRvIII mRNA with heparin reduced transfer of this message to recipient cells. The effect of heparin on EVs uptake may provide a unique tool to study EV function. It may also foster research of heparin or its derivatives as a therapeutic for disease in which EVs play a role.
      datePublished:2013-09-04T00:00:00Z
      dateModified:2013-09-04T00:00:00Z
      pageStart:343
      pageEnd:351
      sameAs:https://doi.org/10.1007/s11060-013-1235-y
      keywords:
         Extracellular vesicles
         Exosomes
         Glioblastoma
         EGFRvIII
         Heparin
         Tumor
         Oncology
         Neurology
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         name:Springer US
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            name:Nadia A. Atai
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                  address:
                     name:Department of Neurology and Program in Neuroscience, Massachusetts General Hospital and Harvard Medical School, Boston, USA
                     type:PostalAddress
                  type:Organization
                  name:University of Amsterdam
                  address:
                     name:Department of Cell Biology and Histology, Academic Medical Center (AMC), University of Amsterdam, Amsterdam, The Netherlands
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Leonora Balaj
            affiliation:
                  name:Massachusetts General Hospital and Harvard Medical School
                  address:
                     name:Department of Neurology and Program in Neuroscience, Massachusetts General Hospital and Harvard Medical School, Boston, USA
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            name:Henk van Veen
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                  address:
                     name:Department of Cell Biology and Histology, Academic Medical Center (AMC), University of Amsterdam, Amsterdam, The Netherlands
                     type:PostalAddress
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                  name:Massachusetts General Hospital and Neuroscience Program, Harvard Medical School
                  address:
                     name:Departments of Neurology and Radiology, Massachusetts General Hospital and Neuroscience Program, Harvard Medical School, Boston, USA
                     type:PostalAddress
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                  address:
                     name:Molecular Imaging Laboratory, MGH/MIT/HMS Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital, Charlestown, USA
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                     name:Harvard Medical School, Boston, USA
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            name:Cornelis J. F. Van Noorden
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                  name:University of Amsterdam
                  address:
                     name:Department of Cell Biology and Histology, Academic Medical Center (AMC), University of Amsterdam, Amsterdam, The Netherlands
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Johan Skog
            affiliation:
                  name:Exosome Diagnostic, Inc.
                  address:
                     name:Exosome Diagnostic, Inc., New York, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Casey A. Maguire
            affiliation:
                  name:Massachusetts General Hospital and Harvard Medical School
                  address:
                     name:Department of Neurology and Program in Neuroscience, Massachusetts General Hospital and Harvard Medical School, Boston, USA
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      volumeNumber:115
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      name:Springer US
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      address:
         name:Department of Neurology and Program in Neuroscience, Massachusetts General Hospital and Harvard Medical School, Boston, USA
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      address:
         name:Department of Cell Biology and Histology, Academic Medical Center (AMC), University of Amsterdam, Amsterdam, The Netherlands
         type:PostalAddress
      name:Massachusetts General Hospital and Harvard Medical School
      address:
         name:Department of Neurology and Program in Neuroscience, Massachusetts General Hospital and Harvard Medical School, Boston, USA
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      address:
         name:Department of Cell Biology and Histology, Academic Medical Center (AMC), University of Amsterdam, Amsterdam, The Netherlands
         type:PostalAddress
      name:Massachusetts General Hospital and Neuroscience Program, Harvard Medical School
      address:
         name:Departments of Neurology and Radiology, Massachusetts General Hospital and Neuroscience Program, Harvard Medical School, Boston, USA
         type:PostalAddress
      name:Massachusetts General Hospital
      address:
         name:Molecular Imaging Laboratory, MGH/MIT/HMS Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital, Charlestown, USA
         type:PostalAddress
      name:Harvard Medical School
      address:
         name:Harvard Medical School, Boston, USA
         type:PostalAddress
      name:University of Amsterdam
      address:
         name:Department of Cell Biology and Histology, Academic Medical Center (AMC), University of Amsterdam, Amsterdam, The Netherlands
         type:PostalAddress
      name:Exosome Diagnostic, Inc.
      address:
         name:Exosome Diagnostic, Inc., New York, USA
         type:PostalAddress
      name:Massachusetts General Hospital and Harvard Medical School
      address:
         name:Department of Neurology and Program in Neuroscience, Massachusetts General Hospital and Harvard Medical School, Boston, USA
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      name:Nadia A. Atai
      affiliation:
            name:Massachusetts General Hospital and Harvard Medical School
            address:
               name:Department of Neurology and Program in Neuroscience, Massachusetts General Hospital and Harvard Medical School, Boston, USA
               type:PostalAddress
            type:Organization
            name:University of Amsterdam
            address:
               name:Department of Cell Biology and Histology, Academic Medical Center (AMC), University of Amsterdam, Amsterdam, The Netherlands
               type:PostalAddress
            type:Organization
      name:Leonora Balaj
      affiliation:
            name:Massachusetts General Hospital and Harvard Medical School
            address:
               name:Department of Neurology and Program in Neuroscience, Massachusetts General Hospital and Harvard Medical School, Boston, USA
               type:PostalAddress
            type:Organization
      name:Henk van Veen
      affiliation:
            name:University of Amsterdam
            address:
               name:Department of Cell Biology and Histology, Academic Medical Center (AMC), University of Amsterdam, Amsterdam, The Netherlands
               type:PostalAddress
            type:Organization
      name:Xandra O. Breakefield
      affiliation:
            name:Massachusetts General Hospital and Neuroscience Program, Harvard Medical School
            address:
               name:Departments of Neurology and Radiology, Massachusetts General Hospital and Neuroscience Program, Harvard Medical School, Boston, USA
               type:PostalAddress
            type:Organization
      name:Peter A. Jarzyna
      affiliation:
            name:Massachusetts General Hospital
            address:
               name:Molecular Imaging Laboratory, MGH/MIT/HMS Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital, Charlestown, USA
               type:PostalAddress
            type:Organization
            name:Harvard Medical School
            address:
               name:Harvard Medical School, Boston, USA
               type:PostalAddress
            type:Organization
      name:Cornelis J. F. Van Noorden
      affiliation:
            name:University of Amsterdam
            address:
               name:Department of Cell Biology and Histology, Academic Medical Center (AMC), University of Amsterdam, Amsterdam, The Netherlands
               type:PostalAddress
            type:Organization
      name:Johan Skog
      affiliation:
            name:Exosome Diagnostic, Inc.
            address:
               name:Exosome Diagnostic, Inc., New York, USA
               type:PostalAddress
            type:Organization
      name:Casey A. Maguire
      affiliation:
            name:Massachusetts General Hospital and Harvard Medical School
            address:
               name:Department of Neurology and Program in Neuroscience, Massachusetts General Hospital and Harvard Medical School, Boston, USA
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Department of Neurology and Program in Neuroscience, Massachusetts General Hospital and Harvard Medical School, Boston, USA
      name:Department of Cell Biology and Histology, Academic Medical Center (AMC), University of Amsterdam, Amsterdam, The Netherlands
      name:Department of Neurology and Program in Neuroscience, Massachusetts General Hospital and Harvard Medical School, Boston, USA
      name:Department of Cell Biology and Histology, Academic Medical Center (AMC), University of Amsterdam, Amsterdam, The Netherlands
      name:Departments of Neurology and Radiology, Massachusetts General Hospital and Neuroscience Program, Harvard Medical School, Boston, USA
      name:Molecular Imaging Laboratory, MGH/MIT/HMS Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital, Charlestown, USA
      name:Harvard Medical School, Boston, USA
      name:Department of Cell Biology and Histology, Academic Medical Center (AMC), University of Amsterdam, Amsterdam, The Netherlands
      name:Exosome Diagnostic, Inc., New York, USA
      name:Department of Neurology and Program in Neuroscience, Massachusetts General Hospital and Harvard Medical School, Boston, USA
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External Links {🔗}(131)

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