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Title:
Heparin blocks transfer of extracellular vesicles between donor and recipient cells | Journal of Neuro-Oncology
Description:
Extracellular vesicles (EVs) have been implicated in tumorigenesis. Biomolecules which can block EV binding and uptake into recipient cells may be of therapeutic value as well as enhance understanding of EV biology. Here, we show that heparin interacts with uptake of tumor-derived as well as non-tumor-derived EVs into recipient cells. Incubation of glioma cell-derived EVs with heparin resulted in micron-sized structures observed by transmission electron microscopy, with EVs clearly visible within these structures. Inclusion of heparin greatly diminished transfer of labeled EVs from donor to recipient tumor cells. We also show a direct interaction between heparin and EVs using confocal microscopy. We found that the block in EV uptake was at the level of cell binding and not internalization. Finally, incubation of glioma-derived EVs containing EGFRvIII mRNA with heparin reduced transfer of this message to recipient cells. The effect of heparin on EVs uptake may provide a unique tool to study EV function. It may also foster research of heparin or its derivatives as a therapeutic for disease in which EVs play a role.
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Keywords {🔍}
article, google, scholar, pubmed, cas, heparin, supplementary, material, cancer, tiff, van, evs, cells, extracellular, tumor, cell, exosomes, usa, recipient, microscopy, access, center, privacy, cookies, content, journal, research, vesicles, balaj, maguire, human, thromb, xob, amsterdam, medical, publish, search, transfer, atai, noorden, uptake, clinical, growth, microvesicles, nat, biol, res, haemost, university, neuroscience,
Topics {✒️}
month download article/chapter syndecan-syntenin-alix regulates mgh/mit/hms athinoula dendritic cell-derived exosomes nih/nci grants ca069246 micron-sized structures observed anti-tumor progression effect glioma cell-derived evs human tumor-derived transmission electron microscopy related subjects van noorden cj henk van veen & cornelis engineered extracellular vesicles full article pdf harvard medical school promote tumor growth privacy choices/manage cookies heparin blocks transfer treating human malignancies tumor-derived evs recipient tumor cells neuro-oncology aims direct interaction �petites cellules” group academic medical center henk van veen van leeuwenhoek center nih grant p30ns045776 heparin reduced transfer glioma-derived evs gene delivery system image analysis core van sluis gl extracellular vesicles cbtrus statistical report distinct biologic roles amplified oncogene sequences thorley-lawson da de gruijl td hettiarachchi rj adjuvant intraportal infusion ornstein dl dos-santos ea de-paiva jf phs grant p30ns045776 massachusetts general hospital scientific advisory board conditions privacy policy article journal
Questions {❓}
- Hettiarachchi RJ, Smorenburg SM, Ginsberg J, Levine M, Prins MH, Büller HR (1999) Do heparins do more than just treat thrombosis?
- Nader HB, Chavante SF, dos-Santos EA, Oliveira TW, de-Paiva JF, Jerônimo SM et al (1999) Heparan sulfates and heparins: similar compounds performing the same functions in vertebrates and invertebrates?
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headline:Heparin blocks transfer of extracellular vesicles between donor and recipient cells
description:Extracellular vesicles (EVs) have been implicated in tumorigenesis. Biomolecules which can block EV binding and uptake into recipient cells may be of therapeutic value as well as enhance understanding of EV biology. Here, we show that heparin interacts with uptake of tumor-derived as well as non-tumor-derived EVs into recipient cells. Incubation of glioma cell-derived EVs with heparin resulted in micron-sized structures observed by transmission electron microscopy, with EVs clearly visible within these structures. Inclusion of heparin greatly diminished transfer of labeled EVs from donor to recipient tumor cells. We also show a direct interaction between heparin and EVs using confocal microscopy. We found that the block in EV uptake was at the level of cell binding and not internalization. Finally, incubation of glioma-derived EVs containing EGFRvIII mRNA with heparin reduced transfer of this message to recipient cells. The effect of heparin on EVs uptake may provide a unique tool to study EV function. It may also foster research of heparin or its derivatives as a therapeutic for disease in which EVs play a role.
datePublished:2013-09-04T00:00:00Z
dateModified:2013-09-04T00:00:00Z
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pageEnd:351
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keywords:
Extracellular vesicles
Exosomes
Glioblastoma
EGFRvIII
Heparin
Tumor
Oncology
Neurology
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headline:Heparin blocks transfer of extracellular vesicles between donor and recipient cells
description:Extracellular vesicles (EVs) have been implicated in tumorigenesis. Biomolecules which can block EV binding and uptake into recipient cells may be of therapeutic value as well as enhance understanding of EV biology. Here, we show that heparin interacts with uptake of tumor-derived as well as non-tumor-derived EVs into recipient cells. Incubation of glioma cell-derived EVs with heparin resulted in micron-sized structures observed by transmission electron microscopy, with EVs clearly visible within these structures. Inclusion of heparin greatly diminished transfer of labeled EVs from donor to recipient tumor cells. We also show a direct interaction between heparin and EVs using confocal microscopy. We found that the block in EV uptake was at the level of cell binding and not internalization. Finally, incubation of glioma-derived EVs containing EGFRvIII mRNA with heparin reduced transfer of this message to recipient cells. The effect of heparin on EVs uptake may provide a unique tool to study EV function. It may also foster research of heparin or its derivatives as a therapeutic for disease in which EVs play a role.
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Extracellular vesicles
Exosomes
Glioblastoma
EGFRvIII
Heparin
Tumor
Oncology
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