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We are analyzing https://link.springer.com/article/10.1007/s11033-025-10373-x.

Title:
MicroRNA-124a/Sirtuin 1 pathway inhibits autophagy to promote hepatocyte apoptosis in acute-on-chronic liver failure | Molecular Biology Reports
Description:
Background The roles of microRNAs in the regulation of autophagy and apoptosis in hepatic cells suggest that they may serve as novel biomarkers or therapeutic targets for various liver injuries. In this study, we aim to analyze whether miR-124a regulates autophagy and apoptosis in hepatic cells, particularly in acute-on-chronic liver failure (ACLF). Materials and methods The plasma and liver tissues from the healthy control (HC) and ACLF patients were included. Moreover, LO2 cells were used to perform in vitro experiments. To measure hepatocyte apoptosis, a TUNEL kit was used. LPS, over-expression or knockdown, 3-methyladenine (3-MA) were used in vitro experiments. The expression levels of the autophagy related proteins (Beclin-1 and LC3), anti-apoptotic proteins (BAX and Bcl-2), Sirtuin 1 (SIRT1), and miR-124a were assessed using western blotting, ELISA, and qRT-PCR. Results ACLF patients had significantly decreased expressions of SIRT1, Bcl-2, LC3, and Beclin-1 and significant upregulation of miR-124a and BAX in both plasma and liver tissues in comparison with the HC group. miR-124a was inversely correlated with autophagy markers and SIRT1, but positively correlated with apoptosis. Upon exposure to LPS, the levels of BAX and miR-124a were notably elevated, while Beclin-1, LC3, SIRT1, and Bcl-2 were notably downregulated in LO2 cells. These changes were further exaggerated in the presence of the miR-124a mimic and EX-527 compared to the miR-124a inhibitor and SRT1720 groups. Co-transfection of miR-124a inhibitor was able to partly counteract the pro-apoptotic effects of the autophagy inhibitor 3-MA. Conclusion miR-124a downregulates SIRT1, thereby suppressing hepatocyte autophagy and consequently inducing apoptosis.
Website Age:
28 years and 1 months (reg. 1997-05-29).

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Custom-built

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๐ŸŒ  Phenomenal Traffic: 5M - 10M visitors per month


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How Does Link.springer.com Make Money? {๐Ÿ’ธ}

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Keywords {๐Ÿ”}

mira, autophagy, apoptosis, liver, sirt, aclf, cells, levels, article, expression, cell, patients, pubmed, google, scholar, lps, fig, inhibitor, bcl, plasma, beclin, study, protein, bax, group, failure, control, groups, cas, hepatocyte, research, significantly, determined, proteins, mimic, samples, ยตgml, tissues, western, analysis, wang, kit, death, injury, blot, data, pathway, vitro, upregulation, clinical,

Topics {โœ’๏ธ}

activating pi3k/akt/gsk-3ฮฒ pathway hrp-conjugated goat anti-rabbit regulating mir-124-3p/sirt1 axis targeting sirt1/ros/jnk pathway screw-cap cryogenic vials article download pdf mir-124a regulates autophagy traditional chinese medicine atgl promotes autophagy/lipophagy cd133โ€‰+โ€‰hepatocellular carcinoma cells mir-124a mimic caused human mir-124a primers mir-124a hampers autophagy mir-124a expression rose mir-124a reduced autophagy mir-124a mimic showed emerging molecular functions lps-induced lo2 cells mir-124a/sirt1 axis pro-apoptotic impact caused enhancing pro-apoptotic factors mir-124a expression levels mir-124a expression level serum mir-124a levels end-stage liver disease mir-124a modulates autophagy mirneasy serum/plasma kit pro-apoptotic protein bax anti-apoptotic protein bcl-2 anti-apoptotic bcl-2 protein hebei medical university mir-124a inhibitors resulted autophagy-related proteins lead predicted mir-124a targets plasma mir-124a levels full access privacy choices/manage cookies search search sirt1-mediated deacetylation autophagy-related protein beclin-1 mir-124-3p inhibitor autophagy-related protein lc3 induced lo2 cells lo2 cells induced mirneasy mini kit mir-124-3p mimic sirt1/foxo1 pathway mir-124a inhibitor sirt1-autophagy pathway mir-124a mimic

Questions {โ“}

  • Allaire M, Rautou PE, Codogno P, Lotersztajn S (2019) Autophagy in liver diseases: time for translation?

Schema {๐Ÿ—บ๏ธ}

WebPage:
      mainEntity:
         headline:MicroRNA-124a/Sirtuin 1 pathway inhibits autophagy to promote hepatocyte apoptosis in acute-on-chronic liver failure
         description:The roles of microRNAs in the regulation of autophagy and apoptosis in hepatic cells suggest that they may serve as novel biomarkers or therapeutic targets for various liver injuries. In this study, we aim to analyze whether miR-124a regulates autophagy and apoptosis in hepatic cells, particularly in acute-on-chronic liver failure (ACLF). The plasma and liver tissues from the healthy control (HC) and ACLF patients were included. Moreover, LO2 cells were used to perform in vitro experiments. To measure hepatocyte apoptosis, a TUNEL kit was used. LPS, over-expression or knockdown, 3-methyladenine (3-MA) were used in vitro experiments. The expression levels of the autophagy related proteins (Beclin-1 and LC3), anti-apoptotic proteins (BAX and Bcl-2), Sirtuin 1 (SIRT1), and miR-124a were assessed using western blotting, ELISA, and qRT-PCR. ACLF patients had significantly decreased expressions of SIRT1, Bcl-2, LC3, and Beclin-1 and significant upregulation of miR-124a and BAX in both plasma and liver tissues in comparison with the HC group. miR-124a was inversely correlated with autophagy markers and SIRT1, but positively correlated with apoptosis. Upon exposure to LPS, the levels of BAX and miR-124a were notably elevated, while Beclin-1, LC3, SIRT1, and Bcl-2 were notably downregulated in LO2 cells. These changes were further exaggerated in the presence of the miR-124a mimic and EX-527 compared to the miR-124a inhibitor and SRT1720 groups. Co-transfection of miR-124a inhibitor was able to partly counteract the pro-apoptotic effects of the autophagy inhibitor 3-MA. miR-124a downregulates SIRT1, thereby suppressing hepatocyte autophagy and consequently inducing apoptosis.
         datePublished:2025-03-15T00:00:00Z
         dateModified:2025-03-15T00:00:00Z
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            miR-124a
            Sirtuin 1
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      headline:MicroRNA-124a/Sirtuin 1 pathway inhibits autophagy to promote hepatocyte apoptosis in acute-on-chronic liver failure
      description:The roles of microRNAs in the regulation of autophagy and apoptosis in hepatic cells suggest that they may serve as novel biomarkers or therapeutic targets for various liver injuries. In this study, we aim to analyze whether miR-124a regulates autophagy and apoptosis in hepatic cells, particularly in acute-on-chronic liver failure (ACLF). The plasma and liver tissues from the healthy control (HC) and ACLF patients were included. Moreover, LO2 cells were used to perform in vitro experiments. To measure hepatocyte apoptosis, a TUNEL kit was used. LPS, over-expression or knockdown, 3-methyladenine (3-MA) were used in vitro experiments. The expression levels of the autophagy related proteins (Beclin-1 and LC3), anti-apoptotic proteins (BAX and Bcl-2), Sirtuin 1 (SIRT1), and miR-124a were assessed using western blotting, ELISA, and qRT-PCR. ACLF patients had significantly decreased expressions of SIRT1, Bcl-2, LC3, and Beclin-1 and significant upregulation of miR-124a and BAX in both plasma and liver tissues in comparison with the HC group. miR-124a was inversely correlated with autophagy markers and SIRT1, but positively correlated with apoptosis. Upon exposure to LPS, the levels of BAX and miR-124a were notably elevated, while Beclin-1, LC3, SIRT1, and Bcl-2 were notably downregulated in LO2 cells. These changes were further exaggerated in the presence of the miR-124a mimic and EX-527 compared to the miR-124a inhibitor and SRT1720 groups. Co-transfection of miR-124a inhibitor was able to partly counteract the pro-apoptotic effects of the autophagy inhibitor 3-MA. miR-124a downregulates SIRT1, thereby suppressing hepatocyte autophagy and consequently inducing apoptosis.
      datePublished:2025-03-15T00:00:00Z
      dateModified:2025-03-15T00:00:00Z
      pageStart:1
      pageEnd:13
      license:http://creativecommons.org/licenses/by-nc-nd/4.0/
      sameAs:https://doi.org/10.1007/s11033-025-10373-x
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         miR-124a
         Sirtuin 1
         Autophagy
         Apoptosis
         Acute-on-chronic liver failure
         Animal Biochemistry
         Animal Anatomy / Morphology / Histology
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                  address:
                     name:Baodi District Hospital of Traditional Chinese Medicine in Tianjin, Tianjin, China
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                     type:PostalAddress
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            name:Chuan Shen
            affiliation:
                  name:The Third Hospital of Hebei Medical University
                  address:
                     name:Department of Infectious Disease, The Third Hospital of Hebei Medical University, Shijiazhuang, China
                     type:PostalAddress
                  type:Organization
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            name:Luyuan Ma
            affiliation:
                  name:The Third Hospital of Hebei Medical University
                  address:
                     name:Department of Infectious Disease, The Third Hospital of Hebei Medical University, Shijiazhuang, China
                     type:PostalAddress
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            type:Person
            name:Caiyan Zhao
            affiliation:
                  name:The Third Hospital of Hebei Medical University
                  address:
                     name:Department of Infectious Disease, The Third Hospital of Hebei Medical University, Shijiazhuang, China
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         name:Baodi District Hospital of Traditional Chinese Medicine in Tianjin, Tianjin, China
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            name:The Third Hospital of Hebei Medical University
            address:
               name:Department of Infectious Disease, The Third Hospital of Hebei Medical University, Shijiazhuang, China
               type:PostalAddress
            type:Organization
      name:Xin Wang
      affiliation:
            name:Baodi District Hospital of Traditional Chinese Medicine in Tianjin
            address:
               name:Baodi District Hospital of Traditional Chinese Medicine in Tianjin, Tianjin, China
               type:PostalAddress
            type:Organization
      name:Yadong Wang
      affiliation:
            name:The Third Hospital of Hebei Medical University
            address:
               name:Department of Infectious Disease, The Third Hospital of Hebei Medical University, Shijiazhuang, China
               type:PostalAddress
            type:Organization
      name:Chuan Shen
      affiliation:
            name:The Third Hospital of Hebei Medical University
            address:
               name:Department of Infectious Disease, The Third Hospital of Hebei Medical University, Shijiazhuang, China
               type:PostalAddress
            type:Organization
      name:Luyuan Ma
      affiliation:
            name:The Third Hospital of Hebei Medical University
            address:
               name:Department of Infectious Disease, The Third Hospital of Hebei Medical University, Shijiazhuang, China
               type:PostalAddress
            type:Organization
      name:Caiyan Zhao
      affiliation:
            name:The Third Hospital of Hebei Medical University
            address:
               name:Department of Infectious Disease, The Third Hospital of Hebei Medical University, Shijiazhuang, China
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Department of Infectious Disease, The Third Hospital of Hebei Medical University, Shijiazhuang, China
      name:Baodi District Hospital of Traditional Chinese Medicine in Tianjin, Tianjin, China
      name:Department of Infectious Disease, The Third Hospital of Hebei Medical University, Shijiazhuang, China
      name:Department of Infectious Disease, The Third Hospital of Hebei Medical University, Shijiazhuang, China
      name:Department of Infectious Disease, The Third Hospital of Hebei Medical University, Shijiazhuang, China
      name:Department of Infectious Disease, The Third Hospital of Hebei Medical University, Shijiazhuang, China

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