We are analyzing https://link.springer.com/article/10.1007/s11010-016-2816-9.

Title:
Involvement of microRNA-141-3p in 5-fluorouracil and oxaliplatin chemo-resistance in esophageal cancer cells via regulation of PTEN | Molecular and Cellular Biochemistry
Description:
microRNAs (miRNAs) act as a major regulator of acquired chemo-resistance in various types of cancer therapeutics. This study investigated the contribution of miRNAs in influencing multiple drug resistance in esophageal squamous cell carcinoma (ESCC). The sensitivity of four ESCC cell lines (EC109, EC9706, TE-1 and KYSE-150) to 5-fluorouracil (5-FU) and oxaliplatin (OX) was determined by MTT assay. A 5-FU and OX-resistant subline, EC9706R, was established by continuous exposure to stepwise increasing concentration of 5-FU and OX. Microarray technology was used to compare the differential expression of miRNAs between resistant cells and parental cells. Chemo-sensitivity assay was performed to evaluate drug response in EC9706R cells transfected with miRNA mimic or inhibitor. The direct targets of miRNA were identified by employing pathway analysis and then confirmed with luciferase assay. Sixty ESCC tissue samples and their paired adjacent normal tissues were collected to validate the expression of identified miRNA. Mouse models were further utilized to investigate the function of miRNA on acquired chemo-resistance. MicroRNA panel results indicated that a total of 12 miRNAs were differentially expressed and miR-141-3p was highly over expressed in resistant cells. Inhibition of miR-141-3p reversed acquired chemo-resistance in EC9706R cells by stimulating apoptosis. The expression of miR-141-3p was significantly increased in ESCC tissue samples compared to their matched distant normal tissues. In addition, the elevated miR-141-3p expression was found to be associated with ESCC differentiation status and TNM stage. Moreover, Phosphatase and tensin homolog (PTEN) was identified as direct target of miR-141-3p. Western blot exhibited altered protein levels of PTEN, Akt, and PI3k with miR-141-3p inhibitor. An inverse correlation between PTEN expression and miR-141-3p expression was also observed in tissue samples. EC9706R xenograft mouse model became sensitized to 5-FU and OX treatment following miR-141-3p inhibitor transfection in vivo. Our study demonstrated that miR-141-3p contributed to an acquired chemo-resistance through PTEN modulation both in vitro and in vivo.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Education
Science
Health & Fitness

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 7,643,078 visitors per month in the current month.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We can't figure out the monetization strategy.

The purpose of some websites isn't monetary gain; they're meant to inform, educate, or foster collaboration. Everyone has unique reasons for building websites. This could be an example. Link.springer.com might be earning cash quietly, but we haven't detected the monetization method.

Keywords {🔍}

pubmed, cancer, article, google, scholar, cas, cell, esophageal, cells, central, carcinoma, pten, resistance, expression, mirp, chemoresistance, drug, wang, squamous, microrna, hong, escc, access, res, human, xian, privacy, cookies, content, research, fluorouracil, chen, yang, mirnas, mirna, pathway, chemotherapy, publish, search, september, acquired, lines, ecr, resistant, akt, downregulation, induces, targeting, med, mol,

Topics {✒️}

small cell lung cancer month download article/chapter oxaliplatin-resistant colorectal cancer cells promotes epithelial-mesenchymal transition renal cell carcinoma proliferation drug resistance-related micrornas brd7-mediated cell proliferation mir-200c induces chemoresistance hong bing ma akt signaling pathway yi-nan ma esophageal cancer chemotherapy esophageal cancers mediated related subjects qing-juan chen enhancing akt signaling mir-141-3p inhibitor transfection epithelial-mesenchymal transition elevated mir-141-3p expression oesophageal carcinoma hong-tao ren escc cell lines full article pdf chemotherapy-induced modification acquired chemo-resistance pten/akt pathway reverse multidrug resistance cell sensitivity test esophageal cancer cells oxaliplatin chemo-resistance controlling drug resistance privacy choices/manage cookies targeting multidrug resistance mir-21 modulates chemosensitivity mir-141-3p contributed pi3k/pten modulation check access instant access mir-141-3p inhibitor employing pathway analysis article jin ovarian cancer cells cellular biochemistry aims mir-141-3p expression esophageal carcinoma article molecular microrna panel results microrna biogenesis pathways human cancers evaluate drug response

Schema {🗺️}

WebPage:
      mainEntity:
         headline:Involvement of microRNA-141-3p in 5-fluorouracil and oxaliplatin chemo-resistance in esophageal cancer cells via regulation of PTEN
         description:microRNAs (miRNAs) act as a major regulator of acquired chemo-resistance in various types of cancer therapeutics. This study investigated the contribution of miRNAs in influencing multiple drug resistance in esophageal squamous cell carcinoma (ESCC). The sensitivity of four ESCC cell lines (EC109, EC9706, TE-1 and KYSE-150) to 5-fluorouracil (5-FU) and oxaliplatin (OX) was determined by MTT assay. A 5-FU and OX-resistant subline, EC9706R, was established by continuous exposure to stepwise increasing concentration of 5-FU and OX. Microarray technology was used to compare the differential expression of miRNAs between resistant cells and parental cells. Chemo-sensitivity assay was performed to evaluate drug response in EC9706R cells transfected with miRNA mimic or inhibitor. The direct targets of miRNA were identified by employing pathway analysis and then confirmed with luciferase assay. Sixty ESCC tissue samples and their paired adjacent normal tissues were collected to validate the expression of identified miRNA. Mouse models were further utilized to investigate the function of miRNA on acquired chemo-resistance. MicroRNA panel results indicated that a total of 12 miRNAs were differentially expressed and miR-141-3p was highly over expressed in resistant cells. Inhibition of miR-141-3p reversed acquired chemo-resistance in EC9706R cells by stimulating apoptosis. The expression of miR-141-3p was significantly increased in ESCC tissue samples compared to their matched distant normal tissues. In addition, the elevated miR-141-3p expression was found to be associated with ESCC differentiation status and TNM stage. Moreover, Phosphatase and tensin homolog (PTEN) was identified as direct target of miR-141-3p. Western blot exhibited altered protein levels of PTEN, Akt, and PI3k with miR-141-3p inhibitor. An inverse correlation between PTEN expression and miR-141-3p expression was also observed in tissue samples. EC9706R xenograft mouse model became sensitized to 5-FU and OX treatment following miR-141-3p inhibitor transfection in vivo. Our study demonstrated that miR-141-3p contributed to an acquired chemo-resistance through PTEN modulation both in vitro and in vivo.
         datePublished:2016-09-19T00:00:00Z
         dateModified:2016-09-19T00:00:00Z
         pageStart:161
         pageEnd:170
         sameAs:https://doi.org/10.1007/s11010-016-2816-9
         keywords:
            Esophageal squamous cell carcinoma
            Chemotherapy
            Resistance
            microRNA
            Cell line
            Xenograft
            Biochemistry
            general
            Cardiology
            Cancer Research
            Medical Biochemistry
         image:
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11010-016-2816-9/MediaObjects/11010_2016_2816_Fig1_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11010-016-2816-9/MediaObjects/11010_2016_2816_Fig2_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11010-016-2816-9/MediaObjects/11010_2016_2816_Fig3_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11010-016-2816-9/MediaObjects/11010_2016_2816_Fig4_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11010-016-2816-9/MediaObjects/11010_2016_2816_Fig5_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11010-016-2816-9/MediaObjects/11010_2016_2816_Fig6_HTML.gif
         isPartOf:
            name:Molecular and Cellular Biochemistry
            issn:
               1573-4919
               0300-8177
            volumeNumber:422
            type:
               Periodical
               PublicationVolume
         publisher:
            name:Springer US
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Ying-ying Jin
               affiliation:
                     name:The Second Affiliated Hospital of Xi’an Jiaotong University
                     address:
                        name:Department of Oncology, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Qing-juan Chen
               affiliation:
                     name:Xian Yang Center Hospital
                     address:
                        name:Department of Oncology, Xian Yang Center Hospital, Xian Yang, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Kun Xu
               affiliation:
                     name:The Second Affiliated Hospital of Xi’an Jiaotong University
                     address:
                        name:Department of Oncology, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Hong-tao Ren
               affiliation:
                     name:The Second Affiliated Hospital of Xi’an Jiaotong University
                     address:
                        name:Department of Oncology, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Xing Bao
               affiliation:
                     name:The Second Affiliated Hospital of Xi’an Jiaotong University
                     address:
                        name:Department of Oncology, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Yi-nan Ma
               affiliation:
                     name:The Second Affiliated Hospital of Xi’an Jiaotong University
                     address:
                        name:Department of Oncology, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Yang Wei
               affiliation:
                     name:The Second Affiliated Hospital of Xi’an Jiaotong University
                     address:
                        name:Scientific Research Center, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Hong bing Ma
               affiliation:
                     name:The Second Affiliated Hospital of Xi’an Jiaotong University
                     address:
                        name:Department of Oncology, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
         isAccessibleForFree:
         hasPart:
            isAccessibleForFree:
            cssSelector:.main-content
            type:WebPageElement
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:Involvement of microRNA-141-3p in 5-fluorouracil and oxaliplatin chemo-resistance in esophageal cancer cells via regulation of PTEN
      description:microRNAs (miRNAs) act as a major regulator of acquired chemo-resistance in various types of cancer therapeutics. This study investigated the contribution of miRNAs in influencing multiple drug resistance in esophageal squamous cell carcinoma (ESCC). The sensitivity of four ESCC cell lines (EC109, EC9706, TE-1 and KYSE-150) to 5-fluorouracil (5-FU) and oxaliplatin (OX) was determined by MTT assay. A 5-FU and OX-resistant subline, EC9706R, was established by continuous exposure to stepwise increasing concentration of 5-FU and OX. Microarray technology was used to compare the differential expression of miRNAs between resistant cells and parental cells. Chemo-sensitivity assay was performed to evaluate drug response in EC9706R cells transfected with miRNA mimic or inhibitor. The direct targets of miRNA were identified by employing pathway analysis and then confirmed with luciferase assay. Sixty ESCC tissue samples and their paired adjacent normal tissues were collected to validate the expression of identified miRNA. Mouse models were further utilized to investigate the function of miRNA on acquired chemo-resistance. MicroRNA panel results indicated that a total of 12 miRNAs were differentially expressed and miR-141-3p was highly over expressed in resistant cells. Inhibition of miR-141-3p reversed acquired chemo-resistance in EC9706R cells by stimulating apoptosis. The expression of miR-141-3p was significantly increased in ESCC tissue samples compared to their matched distant normal tissues. In addition, the elevated miR-141-3p expression was found to be associated with ESCC differentiation status and TNM stage. Moreover, Phosphatase and tensin homolog (PTEN) was identified as direct target of miR-141-3p. Western blot exhibited altered protein levels of PTEN, Akt, and PI3k with miR-141-3p inhibitor. An inverse correlation between PTEN expression and miR-141-3p expression was also observed in tissue samples. EC9706R xenograft mouse model became sensitized to 5-FU and OX treatment following miR-141-3p inhibitor transfection in vivo. Our study demonstrated that miR-141-3p contributed to an acquired chemo-resistance through PTEN modulation both in vitro and in vivo.
      datePublished:2016-09-19T00:00:00Z
      dateModified:2016-09-19T00:00:00Z
      pageStart:161
      pageEnd:170
      sameAs:https://doi.org/10.1007/s11010-016-2816-9
      keywords:
         Esophageal squamous cell carcinoma
         Chemotherapy
         Resistance
         microRNA
         Cell line
         Xenograft
         Biochemistry
         general
         Cardiology
         Cancer Research
         Medical Biochemistry
      image:
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11010-016-2816-9/MediaObjects/11010_2016_2816_Fig1_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11010-016-2816-9/MediaObjects/11010_2016_2816_Fig2_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11010-016-2816-9/MediaObjects/11010_2016_2816_Fig3_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11010-016-2816-9/MediaObjects/11010_2016_2816_Fig4_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11010-016-2816-9/MediaObjects/11010_2016_2816_Fig5_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11010-016-2816-9/MediaObjects/11010_2016_2816_Fig6_HTML.gif
      isPartOf:
         name:Molecular and Cellular Biochemistry
         issn:
            1573-4919
            0300-8177
         volumeNumber:422
         type:
            Periodical
            PublicationVolume
      publisher:
         name:Springer US
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Ying-ying Jin
            affiliation:
                  name:The Second Affiliated Hospital of Xi’an Jiaotong University
                  address:
                     name:Department of Oncology, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Qing-juan Chen
            affiliation:
                  name:Xian Yang Center Hospital
                  address:
                     name:Department of Oncology, Xian Yang Center Hospital, Xian Yang, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Kun Xu
            affiliation:
                  name:The Second Affiliated Hospital of Xi’an Jiaotong University
                  address:
                     name:Department of Oncology, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Hong-tao Ren
            affiliation:
                  name:The Second Affiliated Hospital of Xi’an Jiaotong University
                  address:
                     name:Department of Oncology, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Xing Bao
            affiliation:
                  name:The Second Affiliated Hospital of Xi’an Jiaotong University
                  address:
                     name:Department of Oncology, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Yi-nan Ma
            affiliation:
                  name:The Second Affiliated Hospital of Xi’an Jiaotong University
                  address:
                     name:Department of Oncology, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Yang Wei
            affiliation:
                  name:The Second Affiliated Hospital of Xi’an Jiaotong University
                  address:
                     name:Scientific Research Center, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Hong bing Ma
            affiliation:
                  name:The Second Affiliated Hospital of Xi’an Jiaotong University
                  address:
                     name:Department of Oncology, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
["Periodical","PublicationVolume"]:
      name:Molecular and Cellular Biochemistry
      issn:
         1573-4919
         0300-8177
      volumeNumber:422
Organization:
      name:Springer US
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:The Second Affiliated Hospital of Xi’an Jiaotong University
      address:
         name:Department of Oncology, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
         type:PostalAddress
      name:Xian Yang Center Hospital
      address:
         name:Department of Oncology, Xian Yang Center Hospital, Xian Yang, China
         type:PostalAddress
      name:The Second Affiliated Hospital of Xi’an Jiaotong University
      address:
         name:Department of Oncology, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
         type:PostalAddress
      name:The Second Affiliated Hospital of Xi’an Jiaotong University
      address:
         name:Department of Oncology, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
         type:PostalAddress
      name:The Second Affiliated Hospital of Xi’an Jiaotong University
      address:
         name:Department of Oncology, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
         type:PostalAddress
      name:The Second Affiliated Hospital of Xi’an Jiaotong University
      address:
         name:Department of Oncology, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
         type:PostalAddress
      name:The Second Affiliated Hospital of Xi’an Jiaotong University
      address:
         name:Scientific Research Center, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
         type:PostalAddress
      name:The Second Affiliated Hospital of Xi’an Jiaotong University
      address:
         name:Department of Oncology, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Ying-ying Jin
      affiliation:
            name:The Second Affiliated Hospital of Xi’an Jiaotong University
            address:
               name:Department of Oncology, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
               type:PostalAddress
            type:Organization
      name:Qing-juan Chen
      affiliation:
            name:Xian Yang Center Hospital
            address:
               name:Department of Oncology, Xian Yang Center Hospital, Xian Yang, China
               type:PostalAddress
            type:Organization
      name:Kun Xu
      affiliation:
            name:The Second Affiliated Hospital of Xi’an Jiaotong University
            address:
               name:Department of Oncology, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
               type:PostalAddress
            type:Organization
      name:Hong-tao Ren
      affiliation:
            name:The Second Affiliated Hospital of Xi’an Jiaotong University
            address:
               name:Department of Oncology, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
               type:PostalAddress
            type:Organization
      name:Xing Bao
      affiliation:
            name:The Second Affiliated Hospital of Xi’an Jiaotong University
            address:
               name:Department of Oncology, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
               type:PostalAddress
            type:Organization
      name:Yi-nan Ma
      affiliation:
            name:The Second Affiliated Hospital of Xi’an Jiaotong University
            address:
               name:Department of Oncology, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
               type:PostalAddress
            type:Organization
      name:Yang Wei
      affiliation:
            name:The Second Affiliated Hospital of Xi’an Jiaotong University
            address:
               name:Scientific Research Center, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
               type:PostalAddress
            type:Organization
      name:Hong bing Ma
      affiliation:
            name:The Second Affiliated Hospital of Xi’an Jiaotong University
            address:
               name:Department of Oncology, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Department of Oncology, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
      name:Department of Oncology, Xian Yang Center Hospital, Xian Yang, China
      name:Department of Oncology, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
      name:Department of Oncology, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
      name:Department of Oncology, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
      name:Department of Oncology, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
      name:Scientific Research Center, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
      name:Department of Oncology, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

External Links {🔗}(145)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

CDN Services {📦}

Crossref

4.06s.

LINK . SPRINGER . COM {}